Subclinical Ruminal Acidosis (SARA) and Subclinical Ketosis (SCK) are two of the most prevalent metabolic diseases of dairy cows, with impacts on reproductive performance. There is scarce literature about these diseases in dairy regions in Colombia. In 29 randomly selected herds in Pasto, Colombia, 249 dairy cows were followed weekly for two months postpartum to determine: 1) incidence risk of SARA and SCK; and 2) effects of SARA and SCK on the occurrence of postpartum anestrus (PA) at two months. Samples from ruminal liquor and blood were obtained one time per cow during the first week postpartum to determine presence of SARA (pH < 5.6) and SCK (1.0-2.9 mmol/L of blood Beta-Hydroxy-Butyrate), respectively. PA diagnosis was determined with ultrasound. Pregnancy risks at 30 and 60 days post-breeding (and assumed embryo losses between these days) were determined. Risk factors associated with PA were estimated through a mixed multi-level multivariable logistic regression model, adjusting for clustering of cows within herds. The incidence risks of SARA and SCK were 23.3% and 46.2%, respectively. Simultaneous occurrence of SCK and SARA (SCASCK) was present in 5.2% of the cows. In the final multivariable model, the occurrence of SARA (Odds Ratio: OR = 39.4), SCK (OR = 47.4) and SCASCK (OR = 68.5) was associated with increased odds of PA. Feeding a transition period diet was associated with reduced odds of PA (OR = 0.15). Second parity cows had significantly lower odds of PA than first parity cows (OR = 0.21). In conclusion, inadequate pre-partum and postpartum nutritional management of the herds increased the occurrence of SARA and SCK, which had adverse effects on reproductive performance.

No comprehensive studies have been carried out on the infectious causes of abortion in Kenyan dairy cattle herds. A survey was carried out to determine the seroprevalence of antibodies against Bovine Viral Diarrhoea Virus (BVDV), Brucella abortus (BA) and Neospora caninum (NC) among dairy cattle herds in Nakuru County, a major dairying area in Kenya. A prospective sero-epidemiological study was also undertaken to investigate the effects of BVDV, BA and NC on the occurrence of bovine abortion in dairy cattle herds, where monthly rectal palpations for pregnancy were performed, and monthly serum samples were tested for antibodies to the 3 pathogens. In the 398 randomly selected cattle on 64 dairy herds, the seroprevalences of antibodies to BVDV, NC and BA were 79.1, 25.6 and 16.8%, respectively. Of the cattle seropositive to NC, 83.3% were also seropositive to BVDV and 13.7% to BA. Of the cattle seropositive to BVDV, 17.1% were also seropositive to BA. Among 260 monitored pregnant dairy cattle on the same 64 dairy farms, an incidence risk for abortion of 10.8% (28/260) was identified, while the incidence of other foetal losses was 1.1% (3/260). The incidence rates of sero-conversion for NC, BVD and BA were 1.1, 0.06 and 0.5 new infections/100 cow-months at risk, respectively. The foetal losses were mainly observed in animals less than 96 months old and occurred in mid-gestation. Neospora caninum was associated with most cases (29.0%) of foetal losses, followed by mixed infections of NC and BVDV (12.9%), BVDV (9.9%) and co-infections of BA and NC (6.5%). This is the first study to document the substantial incidence risk of BVDV and NC abortions in dairy cattle in Kenya, and demonstrates the relative importance of BA, BVDV and NC infections in dairy cattle in Kenya. Kenya laboratories should offer diagnostic tests for BVDV and NC to help farmers determine their roles in abortions on their farms. A comprehensive policy on the control of these important diseases should also be put in place by government with the involvement of all stakeholders in the dairy cattle industry.

Animal studies and epidemiological evidence suggest an association between prenatal exposure to drinking water with elevated nitrate (NO₃-N) concentrations and incidence of congenital anomalies. This study used Geographic Information Systems (GIS) to derive individual-level prenatal drinking-water nitrate exposure estimates from measured nitrate concentrations from 140 temporally monitored private wells and 6 municipal water supplies. Cases of major congenital anomalies in Kings County, Nova Scotia, Canada, between 1988 and 2006 were selected from province-wide population-based perinatal surveillance databases and matched to controls from the same databases. Unconditional multivariable logistic regression was performed to test for an association between drinking-water nitrate exposure and congenital anomalies after adjusting for clinically relevant risk factors. Employing all nitrate data there was a trend toward increased risk of congenital anomalies for increased nitrate exposure levels though this was not statistically significant. After stratification of the data by conception before or after folic acid supplementation, an increased risk of congenital anomalies for nitrate exposure of 1.5–5.56 mg/L (2.44; 1.05–5.66) and a trend toward increased risk for >5.56 mg/L (2.25; 0.92–5.52) was found. Though the study is likely underpowered, these results suggest that drinking-water nitrate exposure may contribute to increased risk of congenital anomalies at levels below the current Canadian maximum allowable concentration.