This study utilized integrative bioinformatics tools together with phenotypic assays to understand the whole-genome features of a carbapenem-resistant international clone II Acinetobacter baumannii AB073. Overall, we found the isolate to be resistant to seven antibiotic classes, penicillins, β-lactam/β-lactamase inhibitor combinations, cephalosporins, carbapenems, aminoglycosides, fluoroquinolones, and folate pathway antagonists. These resistance phenotypes are related to various chromosomal-located antibiotic resistance determinants involved in different mechanisms such as reduced permeability, antibiotic target protection, antibiotic target alteration, antibiotic inactivation, and antibiotic efflux. IC2 A. baumannii AB073 could not transfer antibiotic resistance by conjugation experiments. Likewise, mobilome analysis found that AB073 did not carry genetic determinants involving horizontal gene transfer. Moreover, this isolate also carried multiple genes associated with the ability of iron uptake, biofilm formation, immune invasion, virulence regulations, and serum resistance. In addition, the genomic epidemiological study showed that AB073-like strains were successful pathogens widespread in various geographic locations and clinical sources. In conclusion, the comprehensive analysis demonstrated that AB073 contained multiple genomic determinants which were important characteristics to classify this isolate as a successful international clone II obtained from Thailand.

Background and Aim: Antimicrobial resistance (AMR) resulting from antibiotic misuse in livestock poses a growing threat to animal and human health. The development of sustainable probiotic alternatives supports the United Nations Sustainable Development Goals (SDGs) for zero hunger (SDG 2), good health and well-being (SDG 3), and responsible consumption and production (SDG 12). This study aimed to isolate, characterize, and perform genomic analysis of lactic acid bacteria (LAB) from swine feces exhibiting antibacterial activity against pathogenic Escherichia coli, to explore their potential as eco-friendly probiotic feed additives. Materials and Methods: Thirty fecal samples were collected from slaughtered crossbred pigs in Thailand. LAB isolates were screened for antibacterial activity against five E. coli pathotypes (Enteroaggregative E. coli, enterohemorrhagic E. coli, enteroinvasive E. coli, enterotoxigenic E. coli, and enteropathogenic E. coli) and assessed for acid and bile tolerance, adhesion capacity, and gastrointestinal survival. Two promising isolates (ATP111 and ATP210) were subjected to whole-genome sequencing and bioinformatics analyses for genes related to antimicrobial production, stress tolerance, virulence, and AMR. Results: Among 93 initial isolates, Lactiplantibacillus argentoratensis ATP111 and Weissella cibaria ATP210 exhibited broad-spectrum inhibition against all E. coli pathotypes. Both strains survived under pH 2.5 and 1% bile conditions, showing 74.39% and 66.90% survival, respectively, in simulated gastrointestinal conditions. Genomic analyses revealed the presence of genes encoding bacteriocins, polyketide synthases, terpenes, and multiple stress-response proteins, supporting their resilience and antimicrobial functionality. Importantly, both genomes lacked virulence and AMR genes, confirming biosafety for probiotic use. Conclusion: The integrated phenotypic and genomic evidence positions L. argentoratensis ATP111 and W. cibaria ATP210 as safe, effective, and sustainable probiotic candidates for swine health management. Their application as antibiotic alternatives aligns with SDG 3 (good health and well-being), SDG 12 (responsible consumption and production), and SDG 15 (life on land), contributing to reduced antibiotic dependence and improved livestock sustainability. Future in vivo validation is recommended to confirm efficacy and support global AMR mitigation efforts. Keywords: antimicrobial resistance, Escherichia coli, Lactiplantibacillus argentoratensis, Probiotics, SDG 12 (responsible consumption and production), SDG 15 (life on land), SDG 2 (zero hunger), SDG 3 (good health and well-being), sustainability, swine health, Weissella cibaria.

The diversity of environmental yeast communities is underestimated in tropical and sub‐tropical regions. Numerous studies demonstrated that human activity can alter the yeast diversity and increase pathogenic yeast proportions, indicating that people who frequently visit those areas are at risk of being infected. The main purpose of this study was to investigate the abundance and diversity of yeasts obtained from recreation areas in Bangkok. In this study, 158 soil and water samples were collected from 12 public parks. The analysis of the yeast communities revealed different patterns among recreation areas. Moreover, we aimed to identify yeasts using the internal transcribed spacer (ITS) region. Yeast isolates were identified into 22 genera, with Candida being the most common. Although the ITS region may be used to distinguish yeasts at the genus level, some isolates remain unidentified. Thus, our findings are the first report highlighting the diversity of yeast from recreation areas in Bangkok. Our study also provides information on the ITS region for environmental yeast identification, suggesting that this region might be appropriate for some yeast taxa. In conclusion, this study proposes that the abundance and diversity of yeast may differ due to several factors, such as the surrounding environment, park landscapes, and water supplies for park maintenance.