Angola suffered a long-lasting military conflict. Therefore, traditional knowledge of plant usage is still an important part of cultural heritage, especially concerning the still very poor health care system in the country. Our study documents for the first time traditional knowledge of plant use of local Bakongo communities in the northern province of Uíge on a large scale with a focus on medicinal plants and puts data in context to different parameters of age, gender and distance to the provincial capital. Field work was carried out during nine field trips in 13 municipalities between October 2013 and October 2016. In 62 groups, 162 informants were interviewed. Herbarium specimens were taken for later identification. Database was analysed using Relative Frequency of Citations, Cultural Importance Index, and Informant Consensus Factor. Furthermore, significances of influence of age, gender and distance were calculated. Our study presents 2390 use-reports, listing 358 species in 96 plant families, while just three out of 358 mentioned species are endemic to Angola about one-fifth are neophytes. The larger the distance, the higher the number of use citations of medical plants. Although women represent just a fifth of all citations (22%), their contribution to medicinal plants was proportionally even higher (83%) than those of men (74%). Fifty percent of all plants mentioned in the study were just listed by men, 12% just by women. We made some new discoveries, for example. Gardenia ternifolia seems to be promising for treatment of measles, and Annona stenophylla subsp. cuneata has never been ethnobotanically nor phytochemically investigated. While the study area is large, no significant influence of the distance in regard to species composition in traditional healer’s concepts of the respective village was pointed out. Although several plants were just mentioned by women or men, respectively, no significant restriction to gender-specific illnesses in medical plant use could be found. Merely concerning the age of informants, a slight shift could be detected. Visual representation of the ethnobotanical study in Uíge, northern Angola.

Based on data from a previous ethnobotanical study in northern Angola, phytochemical investigations into the methanolic rhizomes and roots extract of Cyperus articulatus, monitored by in vitro assays, resulted in the recovery of 12 sesquiterpenes, 3 stilbenes, 2 phenolic acids, 1 monoterpene, and 1 flavonoid. Among them, 14 compounds were isolated for the first time from this species. Their inhibitory potential against nitric oxide (NO) production, as well as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, was evaluated in LPS-treated J774A.1 murine macrophages. Especially, both stilbene dimer trans-scirpusin B and trimer cyperusphenol B showed promising inhibitory activity against the production of the inflammatory mediator, NO, in a concentration-dependent manner (10–1 µM). The obtained data are the first results confirming the anti-inflammatory potential of C. articulatus and support its indigenous use as a traditional remedy against inflammation-related disorders.

Background: Angola has one of the highest annual under-five mortality rates in in the world and malnutrition poses a severe problem in the country. This study is the first to focus on the traditional knowledge of plants, foods, and treatments used by the local population in the province of Uíge to affect the quality and quantity of human breast milk, since decades of independence and civil war impeded ethnobotanical studies in this area.Methods: This study was conducted in eight municipalities in the province of Uíge, Northern Angola in February and March 2018. In 265 semi-structured interviews, 360 informants in 40 rural villages were asked about plants, food, and treatments used to affect the quality and quantity of human breast milk. Additionally, information on child mortality and the duration of breastfeeding were collected. Whenever possible, plant specimens were collected for later identification. To determine the local importance of the collected plants, food, and treatments, the Relative Frequency of Citations was calculated.Results: Most women reported to have no problems with their breast milk production. The duration of breastfeeding meets the recommendations of the World Health Organization (WHO). Across all use categories, 69 plants from 36 plant families, and 21 other foods and treatments could be identified.Conclusions: The study shows an overview of a variety of plants, foods, and treatments used by mothers as galactagogues, to “clean” or to reduce their breast milk and those which they avoided to use during the lactation period. There is great potential for further research into this traditional knowledge. Also, further analysis of some of the plants could be of interest.

The forests and savannahs of Mucaba municipality in northern Angola hold enormous potential for plant-based non-timber forest products (NTFPs), including wild food plants (WFPs). Mucaba communities have accumulated a wealth of traditional knowledge on the use of wild edible plants over their long history. However, few studies have highlighted this unique and globally attractive culture. The ethnobotanical survey was conducted in 9 villages in Mucaba Municipality in September and October 2020. A total of 65 local informants were interviewed (35 women and 30 men), and their traditional knowledge of wild edible plants was documented. A total of 46 wild edible plants, belonging to 25 families and 30 genera were documented. These plants are consumed as wild edible vegetables, seasonal fruits, spice substitutes for tea, and forest alcoholic drinks. The traditional knowledge of wild edible plants held by the villagers of Mucaba is rich but is in danger of being lost among the younger generation. Therefore, the diversified cultivation of these local plants would allow their sustainable use and conservation especially of the endangered species in this part of Angola.

The use of medicinal plants in the management of microbial infections is significant to the health of the indigenous people in many Angolan communities. The present study provides a comprehensive overview of medicinal plants used for the management of microbial infections in Angola. Relevant information was extracted from research articles published and associated with the use of medicinal plants in the management of microbial infections in Angola (from January 1976 to November 2023). Data or information were gathered from the literature sourced from Wiley Online, SciFinder, Google Scholar, Web of Science, Scopus, ScienceDirect, BMC, Elsevier, SpringerLink, PubMed, books, journals and published M.Sc. and Ph.D. thesis. A total of 27 plant species, representing 19 families, were recorded in this study. Hypericaceae (11%), Lamiaceae (11%), Malvaceae (11%), Phyllanthaceae (11%), Fabaceae (16%) and Rubiaceae (16%) were the most predominant families. The leaves are the most used parts (96%), followed by bark (74%) and root (70%). The data revealed that medicinal plants continue to play significant roles in the management of microbial infections in Angola. In order to explore the benefits of the therapeutic potential of indigenous medicinal plants for diseases related to infections; further scientific research studies are important to produce data on their effectiveness using appropriate test models. This approach might assist with the continuing drive regarding the integration of Angolan traditional medicine within mainstream healthcare systems.

Lippia multiflora Moldenke is widely used in Angola, on the African continent, and beyond for the treatment of many health conditions such as hypertension, enteritis, colds, gastrointestinal disturbances, stomachaches, jaundice, coughs, fevers, nausea, bronchial inflammation, conjunctivitis, malaria, and venereal diseases. However, there is limited literature about the active compounds linked with the reported biological activities. This study aims to assess the chemical profiles, antioxidant properties, and the cytotoxicity effects of the roots, stem bark, and leaves of L. multiflora. Chemical characterization of the crude extracts was assessed through quantification of total phenolic and flavonoid contents followed by Q exactive plus orbitrap™ ultra-high-performance liquid chromatography-mass spectrometer (UHPLC-MS) screening. The correlation between the extracts and the correlation between the compounds were studied using the multivariate analysis. Principal component analysis (PCA) loading scores and principal component analysis (PCA) biplots and correlation plots were used to connect specific compounds with observed biological activities. The antioxidant activities of the crude extracts were carried out in vitro using DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging and reducing power assays, while the in vitro toxicology of the crude extracts was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. A total of twenty constituents were characterized and identified using the UHPLC–Q/Orbitrap/MS. The methanol leaf extract showed the highest antioxidant activity in the DPPH free radical scavenging activity (IC₅₀ = 0.559 ± 0.269 μg/mL); however, the stem bark extract had the highest reducing power (IC_0.5 = 0.029 ± 0.026 μg/mL). High phenolic and flavonoid content was found in the dichloromethane leaf extract (32.100 ± 1.780 mg GAE/g) and stem bark extract (624.153 ± 29.442 mg QE/g), respectively. The results show the stem bark, methanol leaf, and dichloromethane leaf extracts were well-tolerated by the Vero cell line at concentrations up to 50 µg/mL. However, at 100 µg/mL onward, some toxicity was observed in the root, methanol leaf, and dichloromethane leaf extracts. The UHPLC–Q/Orbitrap/MS profiles showed the presence of terpenoids (n = 5), flavonoids (n = 5), phenols (n = 4), alkaloids (n = 3), coumarins (n = 1), fatty acids (n = 1), and organic acids (n = 1). According to several studies, these secondary metabolites have been reported and proven to be the most abundant for antioxidant potential. The identified flavonoids (catechin, quercitrin, and (−)-epigallocatechin) and phenolic compound (6-gingerol) can significantly contribute to the antioxidant properties of different plant parts of L. multiflora. The research findings obtained in this study provide a complete phytochemical profile of various parts of L. multiflora that are responsible for the antioxidant activity using UHPLC–Q/Orbitrap/MS analysis. Furthermore, the results obtained in this study contribute to the scientific information or data on the therapeutic properties of Lippia multiflora and provide a basis for further assessment of its potential as a natural remedy.