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Skin health is heavily affected by ultraviolet irradiation from the sun. In addition, senile skin is characterized by major changes in the collagen, elastin and in the hyaluronan content. Natural products (NPs) have been shown to delay cellular senescence or in vivo aging by regulating age-related signaling pathways. Moreover, NPs are a preferable source of photoprotective agents and have been proven to be useful against the undesirable skin hyperpigmentation. Greek flora harvests great plant diversity with approximately 6000 plant species, as it has a wealth of NPs. Here, we report an extensive screening among hundreds of plant species. More than 440 plant species and subspecies were selected and evaluated. The extracts were screened for their antioxidant and anti-melanogenic properties, while the most promising were further subjected to various in vitro and cell-based assays related to skin aging. In parallel, their chemical profile was analyzed with High-Performance Thin-Layer Chromatography (HPTLC) and/or Ultra-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UPLC-HRMS). A variety of extracts were identified that can be of great value for the cosmetic industry, since they combine antioxidant, photoprotective, anti-melanogenic and anti-aging properties. In particular, the methanolic extracts of Sideritis scardica and Rosa damascena could be worthy of further attention, since they showed interesting chemical profiles and promising properties against specific targets involved in skin aging.
Aimilia Sklirou; Maria Angelopoulou; Aikaterini Argyropoulou; Eliza Chaita; Vasiliki Boka; Christina Cheimonidi; Katerina Niforou; Eleni Mavrogonatou; Harris Pratsinis; Eleftherios Kalpoutzakis; Nektarios Aligiannis; Dimitris Kletsas; Ioannis Trougakos; Alexios Skaltsounis. Phytochemical Study and In Vitro Screening Focusing on the Anti-Aging Features of Various Plants of the Greek Flora. Antioxidants 2021, 10, 1206 .
AMA StyleAimilia Sklirou, Maria Angelopoulou, Aikaterini Argyropoulou, Eliza Chaita, Vasiliki Boka, Christina Cheimonidi, Katerina Niforou, Eleni Mavrogonatou, Harris Pratsinis, Eleftherios Kalpoutzakis, Nektarios Aligiannis, Dimitris Kletsas, Ioannis Trougakos, Alexios Skaltsounis. Phytochemical Study and In Vitro Screening Focusing on the Anti-Aging Features of Various Plants of the Greek Flora. Antioxidants. 2021; 10 (8):1206.
Chicago/Turabian StyleAimilia Sklirou; Maria Angelopoulou; Aikaterini Argyropoulou; Eliza Chaita; Vasiliki Boka; Christina Cheimonidi; Katerina Niforou; Eleni Mavrogonatou; Harris Pratsinis; Eleftherios Kalpoutzakis; Nektarios Aligiannis; Dimitris Kletsas; Ioannis Trougakos; Alexios Skaltsounis. 2021. "Phytochemical Study and In Vitro Screening Focusing on the Anti-Aging Features of Various Plants of the Greek Flora." Antioxidants 10, no. 8: 1206.
The fungal strain was isolated from a soil sample collected in Giza province, Egypt, and was identified as Aspergillusochraceopetaliformis based on phenotypic and genotypic data. The ethyl acetate extract of the fungal strain exhibited promising activity levels against several pathogenic test organisms and through a series of 1H NMR guided chromatographic separations, a new α-pyrone-C-lyxofuranoside (1) along with four known compounds (2–5) were isolated. The planar structure of the new metabolite was elucidated by detailed analysis of its 1D/2D NMR and HRMS/IR/UV spectroscopic data, while the relative configuration of the sugar moiety was determined by a combined study of NOESY and coupling constants data, with the aid of theoretical calculations. The structures of the known compounds—isolated for the first time from A. ochraceopetaliformis—were established by comparison of their spectroscopic data with those in the literature. All isolated fungal metabolites were evaluated for their antibacterial and antifungal activities against six Gram-positive and Gram-negative bacteria as well as against three human pathogenic fungi.
Mostafa Asmaey; Dennis Abatis; Ahmed Abdel-Razek; George Lambrinidis; Ioanna Chinou; Nikolas Fokialakis; Nikolaos Tsafantakis; Mohamed Shaaban; Nektarios Aligiannis. Ochraceopyronide, a Rare α-Pyrone-C-lyxofuranoside from a Soil-Derived Fungus Aspergillus ochraceopetaliformis. Molecules 2021, 26, 3976 .
AMA StyleMostafa Asmaey, Dennis Abatis, Ahmed Abdel-Razek, George Lambrinidis, Ioanna Chinou, Nikolas Fokialakis, Nikolaos Tsafantakis, Mohamed Shaaban, Nektarios Aligiannis. Ochraceopyronide, a Rare α-Pyrone-C-lyxofuranoside from a Soil-Derived Fungus Aspergillus ochraceopetaliformis. Molecules. 2021; 26 (13):3976.
Chicago/Turabian StyleMostafa Asmaey; Dennis Abatis; Ahmed Abdel-Razek; George Lambrinidis; Ioanna Chinou; Nikolas Fokialakis; Nikolaos Tsafantakis; Mohamed Shaaban; Nektarios Aligiannis. 2021. "Ochraceopyronide, a Rare α-Pyrone-C-lyxofuranoside from a Soil-Derived Fungus Aspergillus ochraceopetaliformis." Molecules 26, no. 13: 3976.
Propolis is a resinous substance produced by bees that exhibits antimicrobial, immunostimulatory and antioxidant activity. Its use is common in functional foods, cosmetics and traditional medicine despite the fact that it demonstrates low extraction yields and inconsistency in non-toxic solvents. In this work, a new encapsulation and delivery system consisting of liposomes and cyclodextrins incorporating propolis polyphenols has been developed and characterized. The antioxidant, antimutagenic and antiaging properties of the system under normal and UVB-induced oxidative stress conditions were investigated in cultured skin cells and/or reconstituted skin model. Furthermore, the transcript accumulation for an array of genes involved in many skin-related processes was studied. The system exhibits significant polyphenol encapsulation efficiency, physicochemical stability as well as controlled release rate in appropriate conditions. The delivery system can retain the anti-mutagenic, anti-oxidative and anti-ageing effects of propolis polyphenols to levels similar and comparable to those of propolis methanolic extracts, making the system ideal for applications where non-toxic solvents are required and controlled release of the polyphenol content is desired.
Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants 2021, 10, 420 .
AMA StyleEleni Spanidi, Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Sophia Letsiou, Nektarios Aligiannis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Ioannis Mourtzinos, Aglaia Pappa, Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants. 2021; 10 (2):420.
Chicago/Turabian StyleEleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. 2021. "A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications." Plants 10, no. 2: 420.
Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of Olea europaea L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 μg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real-time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy.
Katerina Gioti; Anastasia Papachristodoulou; Dimitra Benaki; Nektarios Aligiannis; Alexios-Leandros Skaltsounis; Emmanuel Mikros; Roxane Tenta. Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells. Nutrients 2021, 13, 354 .
AMA StyleKaterina Gioti, Anastasia Papachristodoulou, Dimitra Benaki, Nektarios Aligiannis, Alexios-Leandros Skaltsounis, Emmanuel Mikros, Roxane Tenta. Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells. Nutrients. 2021; 13 (2):354.
Chicago/Turabian StyleKaterina Gioti; Anastasia Papachristodoulou; Dimitra Benaki; Nektarios Aligiannis; Alexios-Leandros Skaltsounis; Emmanuel Mikros; Roxane Tenta. 2021. "Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells." Nutrients 13, no. 2: 354.
Chemical composition of propolis depends on the plant source and thus on the geographic and climatic characteristics of the site of collection. The aim of this study was to investigate the chemical profile of Greek and Chinese propolis extracts from different regions and suggest similarities and differences between them. Untargeted ultrahigh-performance liquid chromatography coupled to hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-HRMS) method was developed and 22 and 23 propolis samples from Greece and China, respectively, were analyzed. The experimental data led to the observation that there is considerable variability in terms of quality of the distinctive propolis samples. Partial least squares - discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) models were constructed and allowed the identification of significant features for sample discrimination, adding relevant information for the identification of class-determining metabolites. Chinese samples overexpressed compounds that are characteristic of the poplar type propolis, whereas Greek samples overexpress the latter and the diterpenes characteristic of the Mediterranean propolis type.
Maria-Ioanna Stavropoulou; Aikaterini Termentzi; Konstantinos Kasiotis; Antigoni Cheilari; Konstantina Stathopoulou; Kyriaki Machera; Nektarios Aligiannis. Untargeted Ultrahigh-Performance Liquid Chromatography-Hybrid Quadrupole-Orbitrap Mass Spectrometry (UHPLC-HRMS) Metabolomics Reveals Propolis Markers of Greek and Chinese Origin. Molecules 2021, 26, 456 .
AMA StyleMaria-Ioanna Stavropoulou, Aikaterini Termentzi, Konstantinos Kasiotis, Antigoni Cheilari, Konstantina Stathopoulou, Kyriaki Machera, Nektarios Aligiannis. Untargeted Ultrahigh-Performance Liquid Chromatography-Hybrid Quadrupole-Orbitrap Mass Spectrometry (UHPLC-HRMS) Metabolomics Reveals Propolis Markers of Greek and Chinese Origin. Molecules. 2021; 26 (2):456.
Chicago/Turabian StyleMaria-Ioanna Stavropoulou; Aikaterini Termentzi; Konstantinos Kasiotis; Antigoni Cheilari; Konstantina Stathopoulou; Kyriaki Machera; Nektarios Aligiannis. 2021. "Untargeted Ultrahigh-Performance Liquid Chromatography-Hybrid Quadrupole-Orbitrap Mass Spectrometry (UHPLC-HRMS) Metabolomics Reveals Propolis Markers of Greek and Chinese Origin." Molecules 26, no. 2: 456.
The present study aimed to investigate the metabolic profile, as well as the antioxidant and anti-ageing activities of twenty propolis samples from different regions of Greece. Chemical profiling of methanolic extracts was investigated using HPTLC and 1H-NMR techniques. Their antioxidant activity was evaluated by free radical scavenging methods (DPPH and ABTS), whereas anti-ageing properties were assessed as anti-collagenase activity. Extracts were also investigated in vitro for their ability to inhibit tyrosinase, which is responsible for the oxidation of L-DOPA to dopachrome and the production of melanin. The HPTLC and NMR analysis revealed high variability in the phytochemical profile of the methanolic extracts, with three major groups to be observed: a) Group I, consisting of samples rich in terpenoids, which present low antioxidant but high anti-tyrosinase activity, b) Group II, consisting of samples rich in flavonoids, which form a broad cluster with major similarities at the aromatic region and showed the highest anti-oxidant and anti-collagenase activities and c) Group III, consisting of samples with lower flavonoid content than the samples of Group II, which exhibited moderate antioxidant, anti-collagenase and anti-tyrosinase activities. In conclusion, this study has shown high differentiation on the chromatographic and spectroscopic metabolic profile of Greek propolis samples of different geographical origin, which is also reflected in their biological properties. Their important effects as antioxidant, anti-tyrosinase and anti-collagenase agents make propolis an important potent ingredient in the industry of food supplements and cosmeceuticals. Moreover, a correlation of a particular chemical propolis type to a specific type of biological activity will allow to prepare standardized extracts and develop food supplements and cosmeceuticals possessing the desired pharmacological properties.
Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Antigoni Cheilari; Dimitra Benaki; Konstantinos Gardikis; Ioanna Chinou; Nektarios Aligiannis. NMR metabolic profiling of Greek propolis samples: Comparative evaluation of their phytochemical compositions and investigation of their anti-ageing and antioxidant properties. Journal of Pharmaceutical and Biomedical Analysis 2020, 194, 113814 .
AMA StyleMaria-Ioanna Stavropoulou, Konstantina Stathopoulou, Antigoni Cheilari, Dimitra Benaki, Konstantinos Gardikis, Ioanna Chinou, Nektarios Aligiannis. NMR metabolic profiling of Greek propolis samples: Comparative evaluation of their phytochemical compositions and investigation of their anti-ageing and antioxidant properties. Journal of Pharmaceutical and Biomedical Analysis. 2020; 194 ():113814.
Chicago/Turabian StyleMaria-Ioanna Stavropoulou; Konstantina Stathopoulou; Antigoni Cheilari; Dimitra Benaki; Konstantinos Gardikis; Ioanna Chinou; Nektarios Aligiannis. 2020. "NMR metabolic profiling of Greek propolis samples: Comparative evaluation of their phytochemical compositions and investigation of their anti-ageing and antioxidant properties." Journal of Pharmaceutical and Biomedical Analysis 194, no. : 113814.
As part of our ongoing research on phytoestrogens, we investigated the phytochemical profile and estrogen-like activities of eight extracts from the aerial parts of four Genista species of Greek flora using estrogen-responsive cell lines. Ethyl acetate and methanolic extracts of G. acanthoclada, G. depressa,G. hassertiana, and G. millii were obtained with accelerated solvent extraction and their phytochemical profiles were compared using ultra-high-performance liquid chromatography-high-resolution mass spectrometry (uHPLC-HRMS). Fourteen isoflavonoids, previously isolated from G. halacsyi, were used as reference standards for their identification in the extracts. Thirteen isoflavonoids were detected in both extracts of G. acanthoclada and G. hassertiana, while fewer and far fewer were detected in G. millii and G. depressa, respectively. The ethyl acetate extracts of G. hassertiana and G. acanthoclada displayed 2.45- and 1.79-fold higher, respectively, estrogen-like agonist activity in Ishikawa cells compared to MCF-7 cells at pharmacologically relevant concentrations. Both these extracts, but not that of G. depressa, contained mono- and di-O-β-d-glucosides of genistein as well as the aglycone, all three of which are known to display full estrogen-like activity at lower-than-micromolar concentrations. The possibility of using preparations rich in G. hassertiana and/or G. acanthoclada extracts as a potentially safer substitute for low-dose vaginal estrogen for menopausal symptoms is discussed.
Antigoni Cheilari; Argyro Vontzalidou; Maria Makropoulou; Aggeliki K. Meligova; Nikolas Fokialakis; Sofia Mitakou; Michael N. Alexis; Nektarios Aligiannis. Isoflavonoid Profiling and Estrogen-Like Activity of Four Genista Species from the Greek Flora. Molecules 2020, 25, 5507 .
AMA StyleAntigoni Cheilari, Argyro Vontzalidou, Maria Makropoulou, Aggeliki K. Meligova, Nikolas Fokialakis, Sofia Mitakou, Michael N. Alexis, Nektarios Aligiannis. Isoflavonoid Profiling and Estrogen-Like Activity of Four Genista Species from the Greek Flora. Molecules. 2020; 25 (23):5507.
Chicago/Turabian StyleAntigoni Cheilari; Argyro Vontzalidou; Maria Makropoulou; Aggeliki K. Meligova; Nikolas Fokialakis; Sofia Mitakou; Michael N. Alexis; Nektarios Aligiannis. 2020. "Isoflavonoid Profiling and Estrogen-Like Activity of Four Genista Species from the Greek Flora." Molecules 25, no. 23: 5507.
Rindera graeca is a Greek endemic plant of the Boraginaceae family which has never been studied before. Consequently, this study attempted to phytochemically examine the aerial parts of this species. Nine phenolic secondary metabolites were identified, consisting of seven caffeic acid derivatives and two flavonol glucosides, namely rutin and quercetin-3-rutinoside-7-rhamnoside. These flavonoids, together with rosmarinic acid, were isolated via column chromatography and structurally determined through spectral analysis. Quercetin-3-rutinoside-7-rhamnoside is an unusual triglycoside, which is identified for the first time in Rindera genus and among Boraginaceae plants. This metabolite was further examined with thermal analysis and its 3D structure was simulated, revealing some intriguing information on its interaction with biological membrane models, which might have potential applications in microcirculation-related conditions. R. graeca was also analyzed for its pyrrolizidine alkaloids content, and it was found to contain echinatine together with echinatine N-oxide and rinderine N-oxide. Additionally, the total phenolic and flavonoid contents of R. graeca methanol extract were determined, along with free radical inhibition assays. High total phenolic content and almost complete inhibition at experimental doses at the free radical assays indicate a potent antioxidant profile for this plant. Overall, through phytochemical analysis and biological activity assays, insight was gained on an endemic Greek species of the little-studied Rindera genus, while its potential for further applications has been assessed.
Christos Ganos; Nektarios Aligiannis; Ioanna Chinou; Nikolaos Naziris; Maria Chountoulesi; Tomasz Mroczek; Konstantia Graikou. Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities. Molecules 2020, 25, 3625 .
AMA StyleChristos Ganos, Nektarios Aligiannis, Ioanna Chinou, Nikolaos Naziris, Maria Chountoulesi, Tomasz Mroczek, Konstantia Graikou. Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities. Molecules. 2020; 25 (16):3625.
Chicago/Turabian StyleChristos Ganos; Nektarios Aligiannis; Ioanna Chinou; Nikolaos Naziris; Maria Chountoulesi; Tomasz Mroczek; Konstantia Graikou. 2020. "Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities." Molecules 25, no. 16: 3625.
In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. Finally, the extracts alleviated the UVB-induced up-regulation of MMPs-3, -7 and -9 in a model of reconstituted skin tissue. In conclusion, honey extracts exhibited significant photoprotective and antiaging properties under UVB exposure conditions and thus could be further exploited as promising agents for developing novel and naturally-based, antiaging cosmeceutical products.
Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants 2020, 9, 566 .
AMA StyleAthanasios Karapetsas, Georgia-Persephoni Voulgaridou, Dimitra Iliadi, Ilias Tsochantaridis, Panagiota Michail, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Sofia Karabournioti, Nektarios Aligiannis, Konstantinos Gardikis, Alex Galanis, Mihalis I. Panayiotidis, Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants. 2020; 9 (7):566.
Chicago/Turabian StyleAthanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. 2020. "Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models." Antioxidants 9, no. 7: 566.
Arbuscular mycorrhizal fungi (AMF) are biotrophe microorganisms that establish a symbiotic association with host-plants. These plant-dependent microorganisms have already shown their strong effects concerning plant growth promotion and were reported to stimulate the secondary metabolites (SM) production of their host-plant. In this study, Anchusa officinalis plants, well-known for their therapeutic properties, were analyzed to assess the AMF effects on SM production. Two different experiments were developed on a semi-hydroponic system to compare treated A. officinalis plants with different AMF. In the first investigation, plants were inoculated with Rhizophagus irregularis, cultivated for 30 days and harvested two times. Concerning the second experiment, four different strains of AMF (R. clarus, R. intraradices, R. irregularis and Glomus aggretum) were tested separately on plants and one sampling was performed after one week. Roots and shoots were separated, lyophilized, ground and extracted using ultrasounds with EtOAc and MeOH (35:65 v/v) at 25°C. After centrifugation, the supernatants were removed and evaporated to dryness. Samples were analyzed with UHPLC-HRMS as well as HPTLC and HPLC-DAD-ELSD. Preliminary results of a targeted metabolomic analysis, showed that the concentration of main compound, rosmarinic acid, present in all the treated plants had no statistically difference from the controls. However, a discernable up-regulation and down-regulation of specific minor SMs in colonized plants was observed, suggesting that the aforementioned AMF affect specific biosynthetic pathways. Further experiments and analyses is needed but the cultivation of medicinal plants with AMF looks a promising way to enhance bioactive metabolites with applications in pharmaceutical and cosmeceutical industry.
E Tsiokanos; A Cartabia; N Tsafantakis; I Lalaymia; A Termentzi; S Declerck; Nektarios Aligiannis; N Fokialakis. Arbuscular mycorrhizal fungi as tool in pharmaceutical and cosmeceutical industry for the enhanced production of secondary metabolites of Anchusa officinalis. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-132 .
AMA StyleE Tsiokanos, A Cartabia, N Tsafantakis, I Lalaymia, A Termentzi, S Declerck, Nektarios Aligiannis, N Fokialakis. Arbuscular mycorrhizal fungi as tool in pharmaceutical and cosmeceutical industry for the enhanced production of secondary metabolites of Anchusa officinalis. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-132.
Chicago/Turabian StyleE Tsiokanos; A Cartabia; N Tsafantakis; I Lalaymia; A Termentzi; S Declerck; Nektarios Aligiannis; N Fokialakis. 2019. "Arbuscular mycorrhizal fungi as tool in pharmaceutical and cosmeceutical industry for the enhanced production of secondary metabolites of Anchusa officinalis." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-132.
The healing properties of Mastic resin are well-known in the Mediterranean basin for more than 2500 years. Nowadays, the resin, a PDO product, has been well-studied and showed antimicrobial, anti-inflammatory, and other activities [1]. However, the fruit and leaves of Pistacia lentiscus L. shrub from Chios have not been thoroughly investigated and additionally, they are discarded as considered by-products of the mastic tree cultivation. In continuation of our research [2], this study emphasises on the exploration of the biological properties of P. lentiscus leaves and fruit collected from the region of Mastichochoria. Leaves (36 samples) and fruit (89 samples) were collected from different altitudes and distance from the sea [Fig. 1] during the maturing period. All samples were extracted with various NMR solvents and their NMR metabolic profiling spectra were acquired. In parallel, their antioxidant capacity as well as their enzyme inhibition properties (tyrosinase, elastase, collagenase, hyaluronidase) related to skin whitening, moisture and elasticity were evaluated. Finally, NMR spectral data were processed with SIMCA software (Umetrics) and multivariate statistical analysis (PCA, OPLS-DA) was used to evaluate the quality characteristics of the samples and to identify potential biomarkers. This approach resulted in the detection of more than 40 spectral features (biomarkers) in all of the extracts and facilitated the classification of samples. To the best of our knowledge, this research effort is the first to study the NMR-based chemical profiling of P. lentiscus leaves and fruit from different regions of Chios and correlate their enzyme inhibition activity by Multivariate Data Analysis.
Antigoni Cheilari; P Papalexis; Argyro Vontzalidou; E Dina; I Smyrnioudis; Nektarios Aligiannis. NMR metabolic profiling of Greek Pistacia lentiscus leaves and fruit extracts for the identification of biomarkers with skin beneficial effects. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-110 .
AMA StyleAntigoni Cheilari, P Papalexis, Argyro Vontzalidou, E Dina, I Smyrnioudis, Nektarios Aligiannis. NMR metabolic profiling of Greek Pistacia lentiscus leaves and fruit extracts for the identification of biomarkers with skin beneficial effects. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-110.
Chicago/Turabian StyleAntigoni Cheilari; P Papalexis; Argyro Vontzalidou; E Dina; I Smyrnioudis; Nektarios Aligiannis. 2019. "NMR metabolic profiling of Greek Pistacia lentiscus leaves and fruit extracts for the identification of biomarkers with skin beneficial effects." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-110.
Background Traditional medicines from Northern Chile have not been extensively studied [1]. Nevertheless, they should be considered as a potential source of bioactive compounds, especially in the fields of infectious and neglected tropical diseases, chronic inflammation and multiple drug resistance. Aim Identification of bioactive compounds isolated from plants used in the medicine of Atacama people, following traditional indications. Results A collaborative work within the Atacama community of Taira (Calama, Chile) has led to the collection of 18 medicinal plant species based on their traditional medicinal use. Anti-bacterial screening has resulted in the identification of Aloysia deserticola and Krameria lappacea EtOAc extracts being the most active against the growth of Gram-positive bacteria strains (5-100 µg/mL). After bioguided fractionation, the neolignan (+)-conocarpan from K. lappacea has demonstrated the lowest MICs values (1-5 µg/mL). Regarding the anti-parasitic activity, Artemisia copa and Azorella atacamensis showed IC50 values between 1-10 µg/mL against Plasmodium, Leishmania and Trypanosoma strains. Several sesquiterpene lactones and diterpenes bearing an endoperoxide group were isolated, their biological evaluations are under progress. The EtOAc extract of Fabiana denudata has shown a strong anti-inflammatory effect: it has inhibited 70% of TNFα production at 1 µg/mL. Polymethoxyflavones and sesquiterpenes were isolated, their evaluation is undergoing. Conclusion The Taira community traditional medicine is rich of bioactive compounds, such as neolignans and endoperoxide diterpenes active against the growth of pathogenic bacteria and parasites, respectively, and sesquiterpenes with anti-inflammatory potential. These encouraging results will be completed with deeper mechanistic studies, as well as synergistic evaluation with reference antibiotics.
S Ortiz; M Lëcso-Bornet; C Bonnal; S Houze; A Tahrioui; E Bouffartigues; S Chevalier; A Gioti; R Tenta; T Rose; B Fiebich; K Stathopoulou; M Makropoulou; Nektarios Aligiannis; S Michel; R Grougnet; S Boutefnouchet. Exploring the traditional medicine of Atacama people from Northern Chile as in inestimable source of bioactive compounds. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-316 .
AMA StyleS Ortiz, M Lëcso-Bornet, C Bonnal, S Houze, A Tahrioui, E Bouffartigues, S Chevalier, A Gioti, R Tenta, T Rose, B Fiebich, K Stathopoulou, M Makropoulou, Nektarios Aligiannis, S Michel, R Grougnet, S Boutefnouchet. Exploring the traditional medicine of Atacama people from Northern Chile as in inestimable source of bioactive compounds. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-316.
Chicago/Turabian StyleS Ortiz; M Lëcso-Bornet; C Bonnal; S Houze; A Tahrioui; E Bouffartigues; S Chevalier; A Gioti; R Tenta; T Rose; B Fiebich; K Stathopoulou; M Makropoulou; Nektarios Aligiannis; S Michel; R Grougnet; S Boutefnouchet. 2019. "Exploring the traditional medicine of Atacama people from Northern Chile as in inestimable source of bioactive compounds." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-316.
Natural products have been a source of medicinal agents for thousands of years and the remarkable number of modern drugs that have been derived, are predominantly based on traditional medicine. However, isolation of natural products has always been tedious, as herbal extracts are complicated systems, containing hundreds of chemical entities. It is time and solvent consuming and very often the procedure ends with the re-isolation of known metabolites. Complementary, there has been a pressing need to involve approaches that accelerate the measurement of metabolite levels directly from plant extracts through the implementation of HTS technologies and chemometrics. The goal of PEGASUS is the establishment of a validated analytical chemometrics platform through the elaboration of contemporary chromatographic and spectroscopic techniques (FCPC, HPTLC, LC-HRMS/MS, NMR) along with sophisticated statistical algorithms for the rapid and effective identification of bioactive compounds, prior to their isolation. The last five years, the development of an HeteroCovariance Approach (HetCa algorithms), has been applied at our department for the discovery of bioactive metabolites for several biological targets such as free radicals, enzymes and cancer cell lines [1]-[4]. HetCa is a MATLAB toolbox based on Statistical Total Correlation Spectroscopy (STOCSY) and Statistical Heterospectroscopy (SHY) methodologies. Specifically, HetCa has been applied for the discovery of antioxidant, anti-tyrosinase, anti-acetylcholinesterase, anti-hyaluronidase and cytotoxic against several cancer cell lines natural compounds from plant species belonging to the Greek flora. PEGASUS incorporates for the first time chromatographic, spectroscopic techniques and various bioactivity results along with advanced chemometrics for the rapid identification of bioactive compounds.
Nektarios Aligiannis. PEGASUS: an analytical chemometrics platform for the discovery of bioactive natural compounds. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-201 .
AMA StyleNektarios Aligiannis. PEGASUS: an analytical chemometrics platform for the discovery of bioactive natural compounds. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-201.
Chicago/Turabian StyleNektarios Aligiannis. 2019. "PEGASUS: an analytical chemometrics platform for the discovery of bioactive natural compounds." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-201.
The implementation of high-throughput screening (HTS) technologies has become an indispensable tool for the detection of bioactive constituents, avoiding re-isolation of known compounds, reducing workload and cost. The aim of our study was the establishment of an integrated high-throughput multivariate statistics platform, relying on FCPC, HPTLC, and NMR, for the direct detection and identification of natural compounds with skin whitening properties prior to any isolation. Greek flora - due to its high biodiversity - was used as a source for the collection of selected plant material. Previous in vitro investigation of plant extracts against their tyrosinase inhibition activity revealed nine extracts as the most promising. The selected extracts were fractionated by FCPC using a certain protocol and each fraction was split in 3 equal parts for: i) HPTLC profiling and bioautography, ii) NMR profiling iii) in vitro assay. An integrated HPTLC-based procedure for the tracing of compounds that contributed to tyrosinase inhibitory effect in active fractions was established with the use of multivariate data analysis [1]. Additionally, NMR spectral data were correlated with the results of tyrosinase activity resulting in the identification of bioactive compounds through the combination of the Heterocovariance approach (HetCA) [2] and the statistical total correlation spectroscopy (STOCSY) [3]. The combined data deriving from NMR and HPTLC correlated to the biological activity by the statistically driven approach, revealed flavans, flavonols, phenolic compounds and stilbenoids between the most promising whitening agents, providing a major reduction in workload by direct use of routine information.
Vi Boka; Argyro Vontzalidou; K Stathopoulou; Antigoni Cheilari; D Benaki; E Gikas; E Mikros; Nektarios Aligiannis. A high-throughput multivariate statistics platform for the discovery of tyrosinase inhibitors. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-088 .
AMA StyleVi Boka, Argyro Vontzalidou, K Stathopoulou, Antigoni Cheilari, D Benaki, E Gikas, E Mikros, Nektarios Aligiannis. A high-throughput multivariate statistics platform for the discovery of tyrosinase inhibitors. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-088.
Chicago/Turabian StyleVi Boka; Argyro Vontzalidou; K Stathopoulou; Antigoni Cheilari; D Benaki; E Gikas; E Mikros; Nektarios Aligiannis. 2019. "A high-throughput multivariate statistics platform for the discovery of tyrosinase inhibitors." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-088.
Paliurus spina-christi Mill. (Rhamnaceae) is a much-branched, deciduous, thorny shrub with green fruits that become brown during maturation. The shrub is found in dry slopes in Mediterranean, Southwest and Central Asia and North America. Its common name is ‘Christ’s thorn’ because it is said that its spiny branches were used to make the crown of thorns which had been placed on Christ’s head before his crucifixion. P. ramosissimus Lour., P. orientalis Franch. and P. hemsleyanus Rehder are well known species of the Eastern Asia [1]. Traditionally, P. spina-christi is used for its diuretic and anti-hypercholesterolemic properties, as well as a palliative against gastrointestinal pain [2]-[5]. Thus, it was decided to investigate the shrub’s fruits phytochemically. Powdered mature fruits were exhaustively extracted with cyclohexane, dichloromethane, methanol and water. In order to remove fats and sugars, the dried methanolic extract was subjected to liquid-liquid extraction with c-Hex., EtOAc and water. The EtOAc fraction was initially chromatographed on Si-gel using Vacuum Liquid Chromatography (NP-VLC), followed by prepTLC and NP-HPLC for the final separations and isolation of the pure secondary metabolites. The water phase was treated using adsorption resin technology and the obtained fractions were further chromatographed using Reversed Phase-Solid Phase Extraction (RP-SPE) and RP-HPLC. Structure determination was based on NMR spectroscopy and LC-MS. So far, there have been isolated and identified 12 secondary metabolites, 1 flavonoid-diglycoside, 2 phenolics, 2 triterpenoids, 1 lignan, 3 phytosterols and 3 cyclopeptide alkaloids (CPAs). One of the CPAs, metabolite 8, has been recognized as a new natural product.
V Amountzias; D Abatis; Nektarios Aligiannis. Phytochemical investigation of the fruits of Paliurus spina-christi Mill. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-211 .
AMA StyleV Amountzias, D Abatis, Nektarios Aligiannis. Phytochemical investigation of the fruits of Paliurus spina-christi Mill. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-211.
Chicago/Turabian StyleV Amountzias; D Abatis; Nektarios Aligiannis. 2019. "Phytochemical investigation of the fruits of Paliurus spina-christi Mill." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-211.
Plant derived natural products have an important role in pharmaceutical, cosmeceutical and food supplements industries. Their increasing demand is leading to the over-exploitation of plant resources and to the over-consumption of organic solvents, widely recognized to be of great environmental concern. For this reason, the design of green extraction methods of natural products is currently a key research topic. [1]-[3] In order to address this issue an eco-friendly natural deep eutectic solvents (NADESs) were used instead of organic solvents for the extraction of naphthoquinones from the roots of Alkanna tinctoria Tausch, a Greek endemic plant from Boraginaceae family. More than twenty NADESs containing choline chloride-, and betaine-, as hydrogen bond acceptor combined with different hydrogen bond donors (sugars, organics acids) were investigated for their potential to extract this particular class of compounds. As a result of the statistical evaluation, the most relevant deep eutectic mixture with the highest extraction efficiency was found to be composed of lactic acid and sucrose (LaS). To maximize the extraction yield, a further optimization step of extraction parameters was followed, including the optimum LaS ratio (w/w), the solid-to-solvent ratio, temperature, water content as well as the application of solid phase extraction techniques for the recovery of the naphtoquinone fraction from the NADES extraction solution. Our results revealed the optimized LaS mixture as a valid green alternative for the extraction of naphthoquinones from Alkanna tinctoria. In fact, by using a ratio of lactic acid-sucrose 5:1 (w/w), a solid-to-solvent ratio of 60:1 (w/v) at 40°C, with 30% of water content, targeted compounds were successfully recovered.
E Bossard; N Tsafantakis; Nektarios Aligiannis; N Fokialakis. Natural deep eutectic solvents: an eco-friendly alternative for the extraction of naphthoquinones from Alkanna tinctoria roots. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-193 .
AMA StyleE Bossard, N Tsafantakis, Nektarios Aligiannis, N Fokialakis. Natural deep eutectic solvents: an eco-friendly alternative for the extraction of naphthoquinones from Alkanna tinctoria roots. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-193.
Chicago/Turabian StyleE Bossard; N Tsafantakis; Nektarios Aligiannis; N Fokialakis. 2019. "Natural deep eutectic solvents: an eco-friendly alternative for the extraction of naphthoquinones from Alkanna tinctoria roots." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-193.
Silymarin-enriched extract (SEE) is obtained from Silybum marianum (Asteraceae). Doxorubicin (DXR) is a widely used chemotherapeutical yet with severe side effects. The goal of the present study was to assess the pharmacologic effect of SEE and its bioactive components silibinin and silychristine when administrated alone or in combination with DXR in the human prostate cancer cells (PC-3). PC-3 cells were treated with SEE, silibinin (silybins A and B), silychristine, alone, and in combination with DXR, and cell proliferation was assessed by the MTT assay. Cell cycle, apoptosis, and autophagy rate were assessed by flow cytometry. Expression levels of autophagy-related genes were quantified by qRT-PCR, ELISA and western blot while transmission electron microscopy was performed to reveal autophagic structures. Finally, NMR spectrometry was used to identify specific metabolites related to autophagy. SEE inhibited PC-3 cell proliferation in a dose-dependent manner while the co-treatment (DXR-SEE) revealed an additive cytotoxic effect. Cell cycle, apoptosis, and autophagy variations were observed in addition to altered expression levels of autophagy related genes (LC3, p62, NBR1, Beclin1, ULK1, AMBRA1), while several modifications in autophagic structures were identified after DXR-SEE co-treatment. Furthermore, treated cells showed a different metabolic profile, with significant alterations in autophagy-related metabolites such as branched-chain amino acids. In conclusion, the DXR-SEE co-treatment provokes perturbations in the autophagic mechanism of prostate cancer cells (PC-3) compared to DXR treatment alone, causing an excessive cell death. These findings propose the putative use of SEE as an adjuvant cytotoxic agent.
Katerina Gioti; Anastasia Papachristodoulou; Dimitra Benaki; Sophia Havaki; Apostolos Beloukas; Argyro Vontzalidou; Nektarios Aligiannis; Alexios-Leandros Skaltsounis; Emmanuel Mikros; Roxane Tenta. Silymarin Enriched Extract (Silybum marianum) Additive Effect on Doxorubicin-Mediated Cytotoxicity in PC-3 Prostate Cancer Cells. Planta Medica 2019, 85, 997 -1007.
AMA StyleKaterina Gioti, Anastasia Papachristodoulou, Dimitra Benaki, Sophia Havaki, Apostolos Beloukas, Argyro Vontzalidou, Nektarios Aligiannis, Alexios-Leandros Skaltsounis, Emmanuel Mikros, Roxane Tenta. Silymarin Enriched Extract (Silybum marianum) Additive Effect on Doxorubicin-Mediated Cytotoxicity in PC-3 Prostate Cancer Cells. Planta Medica. 2019; 85 (11/12):997-1007.
Chicago/Turabian StyleKaterina Gioti; Anastasia Papachristodoulou; Dimitra Benaki; Sophia Havaki; Apostolos Beloukas; Argyro Vontzalidou; Nektarios Aligiannis; Alexios-Leandros Skaltsounis; Emmanuel Mikros; Roxane Tenta. 2019. "Silymarin Enriched Extract (Silybum marianum) Additive Effect on Doxorubicin-Mediated Cytotoxicity in PC-3 Prostate Cancer Cells." Planta Medica 85, no. 11/12: 997-1007.
Prostate cancer is the second most commonly diagnosed cancer in men worldwide, which is almost incurable, once it progresses into the metastatic stage. Adriamycin (ADR) is a known chemotherapeutic agent that causes severe side effects. In recent years, studies in natural plant products have revealed their anticancer activities. In particular, Glycyrrhiza glabra enhanced extract (GGE), commonly known as licorice, has been reported to exert antiproliferative properties against cancer cells. In this study, the cytotoxic potential of GGE was assessed in PC-3 cells, when it is administrated alone or in combination with Adriamycin. PC-3 cells were treated with GGE and/or ADR, and the inhibition of cell proliferation was evaluated by the MTT assay. Cell cycle alterations and apoptosis rate were measured through flow cytometry. Expression levels of autophagy-related genes were evaluated with specific ELISA kits, Western blotting, and real-time PCR, while NMR spectrometry was used to identify the implication of specific metabolites. Our results demonstrated that GGE alone or in co-treatment with ADR shows antiproliferative properties against PC-3 cells, which are mediated by both apoptosis and autophagy mechanisms.
Katerina Gioti; Anastasia Papachristodoulou; Dimitra Benaki; Apostolos Beloukas; Argyro Vontzalidou; Nektarios Aligiannis; Alexios-Leandros Skaltsounis; Emmanuel Mikros; Roxane Tenta. Glycyrrhiza glabra-Enhanced Extract and Adriamycin Antiproliferative Effect on PC-3 Prostate Cancer Cells. Nutrition and Cancer 2019, 72, 320 -332.
AMA StyleKaterina Gioti, Anastasia Papachristodoulou, Dimitra Benaki, Apostolos Beloukas, Argyro Vontzalidou, Nektarios Aligiannis, Alexios-Leandros Skaltsounis, Emmanuel Mikros, Roxane Tenta. Glycyrrhiza glabra-Enhanced Extract and Adriamycin Antiproliferative Effect on PC-3 Prostate Cancer Cells. Nutrition and Cancer. 2019; 72 (2):320-332.
Chicago/Turabian StyleKaterina Gioti; Anastasia Papachristodoulou; Dimitra Benaki; Apostolos Beloukas; Argyro Vontzalidou; Nektarios Aligiannis; Alexios-Leandros Skaltsounis; Emmanuel Mikros; Roxane Tenta. 2019. "Glycyrrhiza glabra-Enhanced Extract and Adriamycin Antiproliferative Effect on PC-3 Prostate Cancer Cells." Nutrition and Cancer 72, no. 2: 320-332.
In this study, an integrated process for the recovery of sesamin and sesamolin, two high added-value lignans of sesame oil (SO) was developed, using synchronous extraction and chromatography techniques. The extraction of SO phenolic content was studied using two different extraction techniques: Annular centrifugal extraction (ACE) and centrifugal partition extraction (CPE). The derived data of each experiment were compared in terms of revealing the yields, time, and solvents consumption showing that CPE is the most effective technique, concerning the solvent consumption. The isolation of lignans was achieved using centrifugal partition chromatography (CPC) both on semi-preparative and preparative scale. The biphasic system used for this purpose consisted of the following solvents: n-Hex/EtOAc/EtOH/H2O in proportion 2:3:3:2 (v/v/v/v) and direct recovery of the two major lignans sesamin and sesamolin was achieved. In parallel the CPC analysis resulted in the isolation of four minor lignans of sesame oil, i.e., samin, sesamol, sesaminol, and episesaminol. Structure elucidation of isolated lignans was based on HRMS/MS and NMR experiments. High-performance liquid chromatography (HPLC) was employed for quantitative analysis of the obtained extracts to determine the purity of the isolated compounds as well. The results of this study demonstrated that sesamin and sesamolin were recovered in purity higher than 95%, verifying the effectiveness of the purposed separation methodology. Finally, due to the general application of sesame oil in cosmetic industry, all the pure compounds were evaluated for their tyrosinase, elastase, collagenase, and hyaluronidase inhibition activity.
Dimitris Michailidis; Apostolis Angelis; Nektarios Aligiannis; Sofia Mitakou; Léandros Skaltsounis. Recovery of Sesamin, Sesamolin, and Minor Lignans From Sesame Oil Using Solid Support-Free Liquid–Liquid Extraction and Chromatography Techniques and Evaluation of Their Enzymatic Inhibition Properties. Frontiers in Pharmacology 2019, 10, 723 .
AMA StyleDimitris Michailidis, Apostolis Angelis, Nektarios Aligiannis, Sofia Mitakou, Léandros Skaltsounis. Recovery of Sesamin, Sesamolin, and Minor Lignans From Sesame Oil Using Solid Support-Free Liquid–Liquid Extraction and Chromatography Techniques and Evaluation of Their Enzymatic Inhibition Properties. Frontiers in Pharmacology. 2019; 10 ():723.
Chicago/Turabian StyleDimitris Michailidis; Apostolis Angelis; Nektarios Aligiannis; Sofia Mitakou; Léandros Skaltsounis. 2019. "Recovery of Sesamin, Sesamolin, and Minor Lignans From Sesame Oil Using Solid Support-Free Liquid–Liquid Extraction and Chromatography Techniques and Evaluation of Their Enzymatic Inhibition Properties." Frontiers in Pharmacology 10, no. : 723.
The aim of this study was to assess the antioxidant, photoprotective, and antiaging effects of Greek propolis. Propolis was subjected to n-heptane or methanol extraction. Total phenolic/flavonoid content and antioxidant potential were determined in the extracts. Promising extracts were evaluated for their cytoprotective properties using human immortalized keratinocyte (HaCaT) or reconstituted human skin tissue following exposure to UVB. Assessment of cytotoxicity, DNA damage, oxidative status, and gene/protein expression levels of various matrix metalloproteinases (MMPs) were performed. The propolis methanolic fractions exhibited higher total phenolic and flavonoid contents and significant in vitro antioxidant activity. Incubation of HaCaT cells with certain methanolic extracts significantly decreased the formation of DNA strand breaks following exposure to UVB and attenuated UVB-induced decrease in cell viability. The extracts had no remarkable effect on the total antioxidant status, but significantly lowered total protein carbonyl content used as a marker for protein oxidation in HaCaT cells. MMP-1, -3, -7, and -9, monitored as endpoints of antiaging efficacy, were significantly reduced by propolis following UVB exposure in a model of reconstituted skin tissue. In conclusion, propolis protects against the oxidative and photodamaging effects of UVB and could be further explored as a promising agent for developing natural antiaging strategies.
Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants 2019, 8, 125 .
AMA StyleAthanasios Karapetsas, Georgia-Persephoni Voulgaridou, Manolis Konialis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Nektarios Aligiannis, Petros Bozidis, Anna Goussia, Konstantinos Gardikis, Mihalis I. Panayiotidis, Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants. 2019; 8 (5):125.
Chicago/Turabian StyleAthanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. 2019. "Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models." Antioxidants 8, no. 5: 125.