NEFELI MARIA MAKRI

Ms. NEFELI MARIA MAKRI

orcid

Research Focus & Metrics

Academic Career Length

3Years (2024-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 August 2024 in Genes
Verified
Journal Article
Open access
PDF file
Alexios PolidorosAlexios Polidoros
Alexios Polidoros
INIrini Nianiou-Obeidat
Irini Nianiou-Obeidat
Nikolaos TsakirpaloglouNikolaos Tsakirpaloglou
Nikolaos Tsakirpaloglou
Nestor PetrouNestor Petrou
Nestor Petrou
EDEleftheria Deligiannidou
Eleftheria Deligiannidou
NMNEFELI MARIA MAKRI
NEFELI MARIA MAKRI
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technologies have revolutionized genome editing, significantly advancing the improvement of cultivated crop species. This review provides an overview of genome-edited crops that have either reached the market or received the necessary approvals but are not yet available to consumers. We analyze various genome-editing studies to understand the distribution of different genome-editing systems, the types of site-directed nucleases employed, and the geographical spread of these studies, with a specific focus on global and European contexts. Additionally, we examine the target crops involved. The review also outlines the multiple steps required for the legal acceptance of genome-edited crops within European jurisdictions. We conclude with suggestions for the future prospects of genome-editing research in Europe, aiming to streamline the approval process and enhance the development and adoption of genome-edited crops.

26 January 2026 in SynBio
Verified
Journal Article
Open access
PDF file
Markos MathioudakisMarkos Mathioudakis
Markos Mathioudakis
RARafail Andreou
Rafail Andreou
APAngeliki Maria Papapanou
Angeliki Maria Papapanou
ASArtemis-Chrysanthi Savva
Artemis-Chrysanthi Savva
AIAsimenia Ioannidou
Asimenia Ioannidou
Spyros GkelisSpyros Gkelis
Spyros Gkelis
Wildfires are increasingly frequent and intense due to climate change, resulting in degraded soils with diminished microbial activity, reduced water retention, and low nutrient availability. In many regions, previously restored areas face repeated burning events, which further exhaust soil fertility and limit the potential for natural regeneration. Traditional reforestation approaches such as seed scattering or planting seedlings often fail in these conditions due to extreme aridity, erosion, and lack of biological support. To address this multifaceted problem, this study proposes a living, biodegradable hydrogel that integrates an engineered soil-beneficial microorganism consortium, designed to deliver beneficial compounds and nutrients combined with endemic plant seeds into a single biopolymeric matrix. Acting simultaneously as a biofertilizer, soil conditioner, and reforestation aid, this 3-in-1 system provides a microenvironment that retains moisture, supports microbial diversity restoration, and facilitates plant germination even in nutrient-poor, arid soils. The concept is rooted in circular economy principles, utilizing polysaccharides from food industry by-products for biopolymer formation, thereby ensuring environmental compatibility and minimizing waste. The encapsulated microorganisms, a Bacillus subtilis strain and a Nostoc oryzae strain, are intended to enrich the soil with useful compounds. They are engineered based on synthetic biology principles to incorporate specific genetic modules. The B. subtilis strain is engineered to break down large polyphenolic compounds through laccase overexpression, thus increasing soil bioavailable organic matter. The cyanobacterium strain is modified to enhance its nitrogen-fixing capacity, supplying fixed nitrogen directly to the soil. After fulfilling its function, the matrix naturally decomposes, returning organic matter, while the incorporation of a quorum sensing-based kill-switch system is designed to prevent the environmental escape of the engineered microorganisms. This sustainable approach aims to transform post-wildfire landscapes into self-recovering ecosystems, offering a scalable and eco-friendly alternative to conventional restoration methods while advancing the integration of synthetic biology and environmental engineering for climate resilience.