In order to study the influence of clothing materials on the cooling effect of mine ventilation clothing, the temperatures of human skin and micro-environment under clothing were used as reflection indexes of the cooling effect, and numerical simulations were carried out using fluent software to compare and analyze the temperature changes of human skin and micro-environment under the influence of mine ventilation clothing made of different clothing materials. The results regarding the human skin temperature showed that the modal fiber type resulted in a 0.4 °C lower temperature than the pure cotton type and that the pure cotton type resulted in a 0.6 °C lower temperature than the silica gel type. The temperature comparison of the micro-environment under clothing showed that the modal fiber type resulted in a lower value than the pure cotton type and that the pure cotton type resulted in a lower value than the silicone type; the cooling effect of the three kinds of mine ventilation clothing was modal fiber type > cotton type > silica gel type. In summary, fabric with good permeability and moisture permeability was helpful in improving the cooling effect of mine ventilation clothing.