The study aimed to evaluate the effect of dried apple pomace (DAP) as a feed additive on the enzymatic activity and non-enzymatic compounds belonging to the antioxidant system in cattle rumen fluid. The experiment included 4 Polish Holstein–Friesian cannulated dairy cows and lasted 52 days. The control group was fed with the standard diet, while in the experimental group, 6% of the feedstuff was replaced by dried apple pomace. After the feeding period, ruminal fluid was collected. The spectrophotometric technique for the activity of lysosomal enzymes, the content of vitamin C, polyphenols, and the potential to scavenge the free DPPH radical was used. The enzyme immunoassay tests (ELISA) were used to establish the activity of antioxidants enzymes and MDA. Among the rumen aminopeptidases, a significant reduction (p < 0.01) from 164.00 to 142.00 was observed for leucyl-aminopeptidase. The activity of glycosidases was decreased for HEX (from 231.00 to 194.00) and β-Glu (from 1294.00 to 1136.00), while a significant statistically increase was noticed for BGRD (from 31.10 to 42.40), α-Glu (from 245.00 to 327.00), and MAN (from 29.70 to 36.70). Furthermore, the activity of catalase and GSH (p < 0.01) was inhibited. In turn, the level of vitamin C (from 22.90 to 24.10) and MDA (from 0.36 to 0.45) was statistically higher (p < 0.01). The most positive correlations were observed between AlaAP and LeuAP (r = 0.897) in the aminopeptidases group and between β-Gal and MAN (r = 0.880) in the glycosidases group. Furthermore, one of the most significant correlations were perceived between SOD and AlaAP (r = 0.505) and AcP (r = 0.450). The most negative correlation was noticed between α-Gal and DPPH (r = −0.533) based on these observations. Apple pomace as a feed additive has an influence on lysosomal degradation processes and modifies oxidation–reduction potential in the rumen fluid. Polyphenols and other low-weight antioxidant compounds are sufficient to maintain redox balance in the rumen.

Objectives: Resveratrol (RES) is well documented for its multiple health benefits, with a notable impact on cancer prevention and therapy. This study aimed to evaluate the effect of RES supplementation on oxidative stress in patients with head and neck cancer (HNC) receiving home enteral nutrition (HEN). Methods: This randomized, single-center, open-label study involved 72 adult patients, with 40 completing the intervention. Participants in the intervention group received 400 mg of liposomal RES daily for 12 weeks alongside HEN, while the control group received HEN only. Body composition and oxidative stress markers—including total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione (GSH)—were measured at baseline and after 12 weeks. Results: Significant increases in TAC and SOD activity were observed in both groups. GPx activity increased significantly only in the RES group. MDA levels rose in both groups but were more pronounced in the RES group. GSH levels showed no significant changes. Phase angle (PhA) increased significantly in the RES group, while no significant change was observed in the control group. Conclusions: RES supplementation may enhance antioxidant defenses, as evidenced by increased GPx activity and improvements in TAC and SOD levels, supporting oxidative balance in patients with HNC receiving HEN. The higher MDA levels in the RES group may reflect RES’s dual antioxidant and pro-oxidant activities. Additionally, the observed increase in PhA suggests potential cellular health benefits. These findings highlight the potential of RES as a complementary antioxidant intervention in clinical oncology, warranting further investigation to clarify its therapeutic effects on oxidative stress and cellular health in cancer care.

Biodegradable polymer particles are of considerable importance due to their multiple applications in medical diagnostics and therapy. Spherical-cap particles have been prepared in a very general and simple method by melting a thin polymer fi l m supported on a solid substrate that is in contact with a hydrophilic solvent. The melted polymer forms droplets which transform into solid particles attached to the surface after cooling down the sample. This approach has been demonstrated for polylactide adlayers on glass, which, when melted in glycerol, produce an array of polymer particles supported on the surface. The size of the particles depends on the experimental conditions and ranges from tens of nanometers to several micrometers. The particles can be employed to incorporate guest species, for example, drug molecules or inorganic nanoparticles. This has been confirmed herein through entrapment of an anticancer drug (doxorubicin) and radiogold (Au-198) nanoparticles. The resulting structures have been examined using a number of complementary physicochemical techniques including scanning and transmission electron microscopy, atomic force and optical microscopy as wel l as Raman and fluorescence spectroscopy.

Aging is closely linked to oxidative stress and inflammation. This review provides a critical overview of the antioxidant compounds present in apple pomace and explores how they may mitigate age-related oxidative damage and inflammatory responses. We focus on the nutritional profile of apple pomace including its macro- and micronutrients, with particular focus on polyphenols, such as procyanidin tannins, quercetin glycosides (rutin, quercetin-3-glucoside), phloridzin, dietary fiber, vitamins, and lipids alongside current techniques for isolating its bioactive components. Special attention is given to biological pathways through which these compounds influence aging: redox regulation via Nrf2, inflammatory modulation via NF-κB, and metabolic regulation via AMPK, SIRT1 and PI3K/Akt/mTOR. Evidence from in vitro cellular models (HepG2, CCD-986Sk fibroblasts), in vivo rodent studies and limited human pilot trials is summarized, as well as existing and emerging applications of apple pomace in functional foods, cosmeceuticals, and other sectors. Finally, we discuss the challenges and future opportunities in harnessing this by-product of the food industry. Although clinical data remain limited, preclinical findings support the repurposing of apple pomace as a sustainable functional ingredient contributing to healthier aging and circular economy goals. Future long-term randomized controlled trials are necessary to confirm efficacy in humans.

Aim: This manuscript summarizes the key scientific and practical outcomes of the #DHPSP2024 digital networking event, focusing on emerging trends in digital health technologies, innovations in patient safety, and their implications for improving healthcare delivery. Methods: The #DHPSP2024 event was held from June 18 to 20, 2024, on X (formerly Twitter) and LinkedIn, connecting professionals and stakeholders in digital health and patient safety from different sectors. Data from posts on X and LinkedIn were analyzed for geographical distribution, engagement metrics (impressions, likes, shares), top hashtags, and frequently used terms. A qualitative analysis of the central themes and key online messaging discussions of the network event was also conducted. Results: On X, 2,329 posts by 179 participants from 38 countries generated over 231,000 impressions, with the most activity in Austria, China, and India. LinkedIn engagement included 3,475 likes, 217 comments, and 2,030 shares. Both platforms highlighted core themes such as digital health, patient safety, treatment quality, research on natural compounds, and interdisciplinary collaboration. Online messaging discussions emphasized technologies like telemedicine and artificial intelligence as critical tools for enhancing care delivery and patient safety. Participants also promoted special issues of scientific journals and explored collaborative research opportunities. Conclusions: The #DHPSP2024 event underscored the pivotal role of digital technologies in transforming healthcare, particularly in improving the quality and safety of interventions. The findings demonstrate how digital networking events, grounded in open innovation, foster global research communities, accelerate knowledge exchange, and support the integration of clinically relevant digital solutions. The strong engagement reflects growing interest in leveraging digital platforms to advance health outcomes and professional development. Overall, the event contributed to greater visibility of ongoing research, encouraged interdisciplinary cooperation, and may positively influence both the adoption of innovations in healthcare practice and the dissemination of scientific knowledge.

The study aimed to check the impact of sportsmen’s physical activity on blood coagulation processes and myocardial micro injuries in young adult male sportsmen. Comparing the results of athletes before and after 6 months of the training season, a statistically significant increase in the following parameters: PT (12.03±0.76 vs. 13.51±0.72 s; p<0.01), INR (1.03±0.07 vs. 1.14±0.06; p<0.01), APPT (28.73±3.27 vs. 33.31±5.63 s; p<0.01) and TT (20.62±0.97 vs. 22.49 ±1.41 s; p<0.01) was observed. Analyzing the results between the control group and the study group before training, the following changes were observed: APTT (35.26±6.74 vs. 28.73±3.27 s; p<0.01), HGMCR (750.17±59.23 vs. 569.38±51.24 ng/L; p <0.01) and L-FABP (329.16±92.04 vs. 151.80±70.10 ng/L; p=0.02) and an increase in H-FABP level (2.75±1.32 vs. 4.14±1.30 ng/mL; p<0.01). Comparing the values between the control group and the study group, but after training, an increase in the value for PT (12.88±1.41 vs. 13.51±0.72 s; p = 0.02), INR (1.09±0.13 vs. 1.14±0.06; p=0.02), TT (20.64±1.43 vs. 22.49±1.41 s; p<0.01) and H-FABP (2.75±1.32 vs. 3.94±1.47 ng/L; p<0.01) and decrease in HGMCR activity (750.17±59.23 vs. 581.47±51.25 ng/L; p<0.01) were observed. There was also a strong positive correlation between HGMCR and H-FABP (r = 0.77) and a strong negative correlation between TT and Fb (r = -0.808) and between TT and APTT (r = -0.424). Summarizing our study, we confirm that physical activity in sportsmen, especially overtraining, may have an impact on blood coagulation processes and may also lead to microdamage of the heart muscle.