Background: Aedes aegypti and Aedes albopictus are two important vectors of several important arboviruses, including the dengue, chikungunya, and Zika viruses. Insecticide application is an important approach to reduce vector abundance during Aedes spp.-borne outbreaks in the absence of effective vaccines and treatments. However, insecticide overuse can result in the development of resistance, and careful monitoring of resistance markers is required. Methods: This meta-analysis and systematic review explored the spatial and temporal patterns of insecticide resistance in Asia from 2000 to 2021. PubMed, Scopus, EbscoHost, and Embase were used to enhance the search capability. The random-effects model was applied for the 94 studies that met our inclusion criteria for qualitative synthesis and meta-analysis. Results: Four major insecticides were studied (malathion, dichlorodiphenyltrichloroethane, permethrin, and deltamethrin). Dichlorodiphenyltrichloroethane resistance rates were high in both Ae. aegypti and Ae. albopictus (68% and 64%, respectively). Conversely, malathion resistance was less prevalent in Ae. aegypti (3%), and deltamethrin resistance was less common in Ae. albopictus (2%). Ae. aegypti displayed consistently high resistance rates (35%) throughout the study period, whereas the rate of insecticide resistance in Ae. albopictus increased from 5% to 12%. The rates of the major kdr mutations F1534C, V1016G, and S989P were 29%, 26%, and 22%, respectively. Conclusions: Insecticide resistance in both Ae. aegypti and Ae. albopictus is widespread in Asia, although the rates vary by country. Continuous monitoring of the resistance markers and modification of the control strategies will be important for preventing unexpected outbreaks. This systematic review and meta-analysis provided up-to-date information on insecticide resistance in dengue-endemic countries in Asia.

North Semarang sub-district is located on the coast of the Java Sea. The coastal area is characterized by high salt content on both the ground and the water compare to other areas. The high salt content environment should have limited the breeding of Dengue Hemorrhagic Fever (DHF) vectors; yet, quite high incidents of DHF cases are reported taken place in North Semarang coastal area. The aim of this study was to describe the epidemiology of DHF incidence, characteristic of cases, and bionomics vector in the coastal area of North Semarang sub-district. This study was applied descriptive observational design to analyze samples consisting of 62 dengue cases and 184 houses. The research variables consisted of coordinate of DHF cases, water salinity, House Index (HI), Container Index (CI), and Aedes species. Data were processed using SPSS in a bivariate manner; while, mapping was analyzed spatially using ArcGIS 10.3. A total of 184 houses were surveyed and 55 cases of DHF were identified. Most cases occurred in 6 -16 year age group (47.3%), water salinity ranged from 2-3%, indicating that the water in the coastal area tended to be brackish water. The results of the Pearson Correlation test showed that there was no relationship between HI and Incidence Rate (IR) of DHF in North Semarang District. Aedes aegypti was identified in a positive container, otherwise Aedes albopictus was not found. DHF cases mostly occurred in school age groups, and were distributed in all villages near or far from the beach. DHF vector could breed in areas with little brackish water, so that dengue transmission might occur in this area.

In Indonesia, dengue hemorrhagic fever (DHF) continues to be an issue. Although there have been fewer dengue infections in the past three years, many areas of Indonesia remain dengue endemic. Insecticide use is one DHF prevention and control method that has been used for a long time. Long-term usage of insecticides can cause the vector, Aedes aegypti, to develop resistance. Insecticide resistance to Aedes aegypti in Indonesia was the focus of this study's analysis of the literature review. A systematic review using keywords based on population, interest and context (PICo) was employed in the study strategy. The databases used to find the publications were ScienceDirect, ProQuest, PubMed, Google Scholar, and Garuda from 2018 to 2022. Hence, 40 papers out of the 6,429 total articles were relevant and should be reviewed and analyzed. According to an analysis of the paper, it was discovered that Aedes aegypti mosquitoes are resistant to insecticides of the pyrethroid and organophosphate classes in various parts of Indonesia. However, it was discovered that several regions were both tolerant of and vulnerable to pyrethroids and organophosphates. If insecticides are to be utilized intolerant and vulnerable locations, monitoring and evaluation of their usage must be done, and a strategy of varying insecticide kinds and dosages and boosting community empowerment toward mosquito nest elimination behaviour must be implemented.

Longevity and development of Aedes aegypti larvae to imago in domestic sewage water