Sulla is a tanniferous legume species largely used as fresh or preserved forage in the ruminants’ diets. Due to its content in polyphenols with antioxidant activity, especially condensed tannins (CT), fresh sulla forage (FSF), when eaten by ruminants, is able to enrich animal products with antioxidant molecules and polyunsaturated fatty acids (PUFA) that are beneficial for consumers’ health. Dehydration represents a valid alternative to haymaking to preserve these properties also in periods when FSF is unavailable. In this research, the effects of ewes’ diets based on sulla hay (SH), pelleted dehydrated sulla forage (DSF) or FSF were evaluated on cheese physico-chemical traits, including fatty acid (FA) profile, vitamins A and E, polyphenols, antioxidant capacity and oxidative stability. The individual daily milk from 10 first lambing (FL) and 10 third lambing (TL) Valle del Belìce ewes at about 60 days in milk, divided homogeneously into 5 groups fed different diets in a partial 5 × 2 Latin square design with 2 phases, and the bulk milk of farming ewes fed only on natural pasture, were used in 2 sessions of micro cheese-making to manufacture cheeses, sampled at 48 h of ripening. The experimental diets were: SHL = SH ad libitum; DSF2 = 2 kg/day DSF per head plus SH ad libitum; FSF2 = 2 kg/day FSF per head plus SH ad libitum; FSF4 = 4 kg/day FSF per head plus SH ad libitum; FSFL = FSF ad libitum. Concentrate was supplied at the amount of 0.8 or 1.2 kg/day per head for FL and TL, respectively. Cheese health properties greatly improved with FSFL diet, based on the exclusive use of fresh forage, that induced the increase in the content of molecules with antioxidant activity, as vitamin A, vitamin E and polyphenols, enhancing the oxidative stability, and the level of PUFA as rumenic acid (C18:2 c9t11) and α-linolenic acid (ALA, C18:3n-3). Cheeses from the DSF2 group showed levels of vitamin A, vitamin E and PUFA higher than SHL cheeses and comparable to those of FSF4 cheeses, whereas their ALA content was not different from that of FSFL cheeses. Evaluating those molecules affected by the level of fresh forage in the diet for their ability to trace the animals’ feeding regime, ALA and its ratio with linoleic acid (LA, C18:2n-6) (LA/ALA) exhibited an effective role as biomarker in discriminating cheese from animals fed fresh forage-based diets. The results showed how dehydrated sulla pellets, as an alternative to hay in periods of limited availability of fresh forage, can preserve the nutritional and health properties of dairy products with regards to their antioxidant capacity and FA profile.

The rising interest in healthier meat options prompted the exploration of alternatives to traditional pork-based products, incorporating meat from different livestock species, feeding regimens, and functional ingredients. This study investigates the production of healthier meat products by examining the physicochemical traits, fatty acid profile, and sensory properties of mortadella made with Cinisara meat of four young bulls and four adult cows, and four females of the Nebrodi Black Pig. All the animals were fed principally on natural resources. Nutritional analysis revealed different levels of moisture, protein, fat, and ash in raw materials, with pistachios contributing to a healthy fatty acid profile rich in monounsaturated and polyunsaturated fatty acids. Formulations using cow meat exhibited higher fat content and caloric value, resulting in sensory attributes such as more intense color, improved fat cube adhesion, and pronounced odors compared to young bull and control mortadella. Fatty acid analysis demonstrated distinctive profiles influenced by the meat type used and, as expected, bovine products showed higher contents of rumenic and other conjugated linoleic acids. Pork mortadella displayed greater ω6 and ω3 values, with a healthier ω6/ω3 ratio comparable to those found in cow products. Young bull mortadella showed the worse atherogenic and thrombogenic indices. The findings underscore the impact of raw materials on the nutritional and sensory attributes of mortadella, emphasizing the necessity for interventions to enhance fatty acid composition in processed meat products.

The high degradability of crude protein (CP) from fresh grass can impair its utilization in ruminants. The presence of a moderate level of polyphenolic substances in the diet of grazing animals could help to overcome this problem. The study aimed to evaluate the effects of supplementation with two varieties of faba bean, with different polyphenol contents, on milk yield and quality, as well as on grazing behaviour, in Cinisara grazing cows. A total of 30 cows, homogeneous per days in milk (61 ± 29) and milk yield (12.9 kg ± 3.2), fed on a mixed pasture (CP 17.9% DM) and supplied with grains (an equal mixture of barley, oats, and wheat) and mixed hay (CP 10.2% DM), were assigned to three dietary treatments and supplemented as follows: control group (C, 10 cows), 1 kg of hay, 6 kg of grains mixture, and 0.4 kg soybean meal; low polyphenols group (LP, 10 cows), 1 kg of hay, 4 kg of grains mixture, and 2 kg/d of faba bean var. Torrelama (total polyphenols 4.4 mg GAE/g DM; CP 28.4% DM); high polyphenols group (HP, 10 cows), 2 kg of hay, 4 kg of grains mixture, and 2 kg/d of faba bean var. Fanfare (total polyphenols 16.4 mg GAE/g DM; CP 28.9% DM). All groups were allowed to graze for 20 h/d on natural pasture forage. The HP supplement tended to increase the milk yield compared to that of the LP and C groups (17.1 vs. 15.3 and 14.6 kg/d, respectively; p = 0.057) but reduced the protein (3.20 vs. 3.39 and 3.47%; p = 0.009) and casein proportions (2.45 vs. 2.67 and 2.74%, respectively; p = 0.007) compared to those from the LP and C treatments. HP milk also showed a higher milk urea nitrogen (MUN) value compared to that of C milk, while an intermediate level was measured in LP milk (25.5 vs. 22.9 and 20.9 mg/dl, respectively; p = 0.036). No dietary effect was evident in the milk fatty acid profile. Eating time at pasture and biting rate were not affected by supplementation. The results do not seem to suggest a difference in the efficiency of use of supplements with different polyphenol contents. In any case, they seem to demonstrate that the use of faba beans, regardless of their polyphenol content, represents a valid alternative to soyabeans, also taking into account the tendency for an increased milk yield found by integrating pasture grazing with faba beans possessing a high level of polyphenols, without significant worsening of the qualitative characteristics of the milk or negatively affecting grazing behaviour.

In Sicilian forage systems, the introduction of native self-seeding annual legumes can be beneficial in low-input farming. Intercropping would be a valuable strategy for implementing pasture resources in Sicilian forage systems during late spring. The aim of this study was to evaluate the effects on ewes’ milk production of continuous grazing with two different mixtures (i) sulla (Sulla coronarium L.), burr medic (Medicago polymorpha L.), and chicory (Cichorium intybus L.) (SuBuCh); and (ii) barrel medic (Medicago truncatula Gaertn.), snail medic (Medicago scutellata L.), and burr medic (BuSnBa). Twenty lactating ewes were homogeneously divided into four groups of five ewes. Each group was assigned to one of four 1500 m² grazing sectors consisting of two replicates of SuBuCh and BuSnBa. Ewes fed with the SuBuCh mixture showed higher milk yield, higher protein (5.17 vs. 4.85%, p < 0.001) and casein content (4.02 vs. 3.73%, p < 0.001), lower urea content (37.70 vs. 45.38 mg dL⁻¹, p < 0.001), and better clotting parameters compared to ewes grazing on the BuSnBa mixture. Finally, ewes in the SuBuCh group showed a smaller decrease in live weight at the end of the grazing period compared to BuSnBa ewes (−2.05 vs. −3.55 kg, respectively), although the difference did not reach a significant level. These preliminary one-year results seem to highlight the promising role of SuBuCh intercrop leading to a potential quantitative/qualitative improvement in grazing resources and the productive performance of grazing ewes in a semi-arid Mediterranean environment. However, it is of note that these outcomes might undergo variations when subjected to a prolonged trial extending beyond three years.

Enhancing forage protein is key to sustainable ruminant nutrition. The nutritive value of Hedysarum coronarium L. was investigated by studying different preservation systems (fresh vs. dehydrated pellet vs. hay) (Exp. 1) and morphological fractions (flowers vs. leaves vs. stems) (Exp. 2). For the fresh and pelleted systems, two cuts were used. Proximately, total polyphenols and condensed tannins were detected. In vitro fermentation characteristics were studied by incubating samples with buffered sheep rumen fluid, estimating methane production by volatile fatty acids. Fresh and pelleted sulla were more nutritionally advantageous than hay, in terms of metabolizable energy and protein-to-fiber ratio. Pelleting at the beginning of flowering proved to be a suitable forage for sheep feeding due to protein (15.1% DM), metabolizable energy (9.64 MJ/kg DM), structural carbohydrates (39.5% DM), and total polyphenols (13.5 GAE g/kg DM) content. This cut showed an in vitro fermentation rate (9.86 mL/h), organic matter degradability (55.7%), and volatile fatty acids (87.3 mmoL/g) that were higher (p < 0.05) than hay. Flowers and leaves showed higher (p < 0.05) levels of secondary metabolites than stems as well as lower methane production. These results suggest the potential influence of these compounds in reducing rumen emissions. Dehydration and pelleting resulted in an effective preservation method for maintaining nutrients in sulla forage.