The current study is aimed to assess water use efficiency and evaluate economic viability of hydroponic and conventional production of barley green fodder by keeping in view the water scarcity challenges in Saudi Arabia. A hydroponic system and open field experimental plot was used to evaluate the water use efficiency for different irrigation regimes. Economic indicators for both production systems are estimated and compared to accomplish economic assessment. Estimated indicators include returns from inputs and net profit; benefit-cost ratio; break-even levels of prices, production, and yield; returns over variable cost; and returns on investment. Results indicated that the yield of barley green fodder produced under hydroponic conditions overtopped the yield under conventional cultivation. Under hydroponic and conventional conditions, WUE was decreased with increasing the harvesting date. However, WUE for the hydroponic technique was much higher than the conventional one. The returns and net profits supported the conventional cultivation methods, where lower dry matter content coupled with higher fixed and variable costs incurred by the hydroponic technique outweighed returns leading to economic loss. Cost-benefit ratios, returns over investment, and break-even prices and yield suggested that growing barley fodder under the hydroponic technique is economically not suitable for small-scale farming. However, regarding water conservation, hydroponic barley cultivation showed superiority over conventional field cultivation. Further research on the adoption of hydroponic fodder cultivation is highly recommended for water-scarce arid regions, such as the Kingdom of Saudi Arabia.

Dioecism has always been an issue in many plant species with its numerous disadvantages, especially in woody trees such as date palms. As one of the most important crops in the Middle Eastern countries, researchers are having problems identifying of sex of the plant in its early stages of development. Hence, proper population stands in the male: female ratio for maintenance is almost impossible in the field for better production. In this study, sex determination of date palm (Phoenix dactilyfera L.) were identified in regions of the Y chromosome (Date-SRY) gene, the pivotal gene that initiates sex determination, using a new technique and thus an economically desirable objective, which will significantly impact profits in seed based cultivations. Partial sequences of the Date-SRY were taken and amplified by nested polymerase chain reaction (PCR). According to the results, the exact sex of date palm was identified in all the tested plants, while amplified regions of the Date-SRY gene closely matched with the human and papaya sequences. In addition, a primer pair was designed to amplify the sequences of the SRY-date gene with confidence that it will identify male date palms. These primer sequences include SRY-date Forward 5'- cggccctctaagtatctgtgcgcaacg-3' (SRY-date F) and the SRY-date Reverse 5'- gtttgcacttcgaagcagag-3' (SRY-date R). The complete sequence of the DNA has been registered and deposited in GenBank (BankIt1598036 DPSRY1 KC577225 thenKJ873056).

Background: Soil salinity causes huge economic losses to agriculture productivity in arid and semiarid areas worldwide. The affected plants face disturbances in osmotic adjustment, nutrient transport, ionic toxicity and reduced photosynthesis. Conventional breeding approaches produce little success in combating various stresses in plants. However, non-conventional approaches, such as in vitro tissue culturing, produce genetic variability in the development of salt-tolerant plants, particularly in woody trees. Results: Embryogenic callus cultures of the date palm cultivar Khalas were subjected to various salt levels ranging from 0 to 300 mM in eight subcultures. The regenerants obtained from the salt-treated cultures were regenerated and evaluated using the same concentration of NaCl with which the calli were treated. All the salt-adapted (SA) regenerants showed improved growth characteristics, physiological performance, ion concentrations and K⁺/Na⁺ ratios than the salt non-adapted (SNA) regenerants and the control. Regression between the leaf Na⁺ concentration and net photosynthesis revealed an inverse nonlinear correlation in the SNA regenerants. Leaf K⁺ contents and stomatal conductance showed a strong linear relationship in SA regenerants compared with the inverse linear correlation, and a very poor coefficient of determination in SNA regenerants. The genetic fidelity of the selected SA regenerants was also tested using 36 random amplified polymorphic DNA (RAPD) primers, of which 26 produced scorable bands. The primers generated 1–10 bands, with an average of 5.4 bands per RAPD primer; there was no variation between SA regenerants and the negative control. Conclusion: This is the first report of the variants generated from salt-stressed cultures and their potential adaptation to salinity in date palm cv. Khalas. The massive production of salt stress-adapted date palm plants may be much easier using the salt adaptation approach. Such plants can perform better during exposure to salt stress compared to the non-treated date palm plants.

Water scarcity has necessitated the adoption of water-saving techniques in both protected and non-protected farming. This study aimed to evaluate the performance of a water-saving soilless cultivation technique and compare it to conventional soil-based cultivation in protected farming. The soilless technique utilized local gravel and a mixture of peat moss, humin-substrate, and perlite in a 4:3:1.5 ratio. During the tomato growth cycle, three irrigation regimes were imposed using drip irrigation: 8 Lh⁻¹ design discharge (D1) emitters, 6 Lh⁻¹ design discharge (D0.75) emitters, and 4 Lh⁻¹ design discharge (D0.5) emitters for both cultivation methods. Vegetative growth, fruit yield, and water consumption were measured and water productivity was determined. Additionally, an economic assessment was conducted by estimating and comparing economic coefficients for both cultivation methods. Estimated coefficients included revenues, net profit, benefit–cost ratio, breakeven levels of production and prices, revenues over variable cost, and revenues on investment. The tomato fruit yield under soil-based cultivation surpassed the yield under soilless cultivation. Water productivity under soilless cultivation was nearly double (24.3 kg m⁻³) that of soil-based cultivation (15.5 kg m⁻³). Soilless cultivation saved 50% of the irrigation water applied by the conventional soil-based method, conserving energy and protecting the soil from deterioration. Revenues and net profits, driven by higher yield and lower variable costs, favored soil-based cultivation. The economic assessment demonstrated that both cultivation methods were economically viable. However, the soil-based cultivation method was more profitable due to its higher fruit yield. Overall, the results of this study suggest that the soilless cultivation technique is a feasible option for water-saving cultivation. However, the soil-based cultivation method remains more profitable due to its superior fruit yield. The soilless cultivation technique offers significant water savings but needs further improvements to achieve comparable economic returns to traditional farming.

Salinity is a serious factor limiting the productivity of agricultural plants. One of the potential problems for plants growing under saline conditions is the inability to up take enough K+. The addition of K+ may considerably improve the salt tolerance of plants grown under salinity. It is assumed that increasing the K+ supply at the root zone can ameliorate the reduction in growth imposed by high salinity. The present study aims to determine whether an increase in the K/Na ratio in the external media would enhance the growth of date palm seedlings under in vitro saline conditions. Date palm plants were grown at four concentrations of Na + K/Cl (mol/m3) with three different K/Na ratios. The 12 salt treatments were added to modified MS medium. The modified MS medium was further supplemented with sucrose at 30 g/l. Growth decreased substantially with increasing salinity. Growth expressed as shoot and root weight, enhanced significantly with certain K/Na ratios, and higher weight was maintained in the presence of equal K and Na. It is the leaf length, leaf thickness and root thickness that had significant contribution on total dry weight. Na+ contents in leaf and root increased significantly increased with increasing salinity but substantial decreases in Na+ contents were observed in the leaf and root with certain K/Na ratios. This could be attributed to the presence of a high K+ concentration in the media. The internal Na+ concentration was higher in the roots in all treatments, which might indicate a mechanism excluding Na+ from the leaves and its retention in the roots. K/Na ratios up to one significantly increased the leaf and root K+ concentration, and it was most pronounced in leaves. The K+ contents in leaf and root was not proportional to the K+ increase in the media, showing a high affinity for K+ uptake at lower external K+ concentrations, but this mechanism continues to operate even with high external Na+ concentrations. Increasing K/Na ratios in the growing media of date plam significantly reduced the absorption of Na+ less than 200 mM and also balance ions compartmentalization.