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Luqing Zheng
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

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Journal article
Published: 06 January 2021 in Agronomy
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Transcriptional regulation is involved in responding to cadmium (Cd) stress in plants. However, the molecular mechanisms of Cd stress responses regulated by transcription factors remain largely unknown in plants. In this study, a rice (Oryza sativa) NAC (no apical meristem [NAM]; Arabidopsis transcription activation factor [ATAF]; cup-shaped cotyledon [CUC]-related) family transcription factor, OsNAC300, was isolated and functionally characterized for its involvement in Cd stress responses and tolerance. OsNAC300 was localized to the nucleus. OsNAC300 was mainly expressed in roots and significantly induced by Cd treatment. Knockout of OsNAC300 resulted in increased sensitivity to Cd stress, while its overexpression lines enhanced tolerance to Cd stress. RNA-Seq analysis revealed that the mutant is impaired in regulating some important genes that were responsive to Cd stress in wild-type rice, such as the pathogenesis-related genes 10a (OsPR10a), OsPR10b, chalcone synthase 1 (OsCHS1), and several others, which was validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. Moreover, chromatin immunoprecipitation-qPCR assays and luciferase reporter assays demonstrated that OsNAC300 directly binds to the promoters of OsPR10a, OsPR10b, and OsCHS1 and activates their transcription. Overall, OsNAC300 is an important regulatory factor in Cd stress responses and tolerance in rice.

ACS Style

Shubao Hu; Kamran Iqbal Shinwari; Yuxinrui Song; Jixing Xia; Heng Xu; Binbin Du; Le Luo; Luqing Zheng. OsNAC300 Positively Regulates Cadmium Stress Responses and Tolerance in Rice Roots. Agronomy 2021, 11, 95 .

AMA Style

Shubao Hu, Kamran Iqbal Shinwari, Yuxinrui Song, Jixing Xia, Heng Xu, Binbin Du, Le Luo, Luqing Zheng. OsNAC300 Positively Regulates Cadmium Stress Responses and Tolerance in Rice Roots. Agronomy. 2021; 11 (1):95.

Chicago/Turabian Style

Shubao Hu; Kamran Iqbal Shinwari; Yuxinrui Song; Jixing Xia; Heng Xu; Binbin Du; Le Luo; Luqing Zheng. 2021. "OsNAC300 Positively Regulates Cadmium Stress Responses and Tolerance in Rice Roots." Agronomy 11, no. 1: 95.

Original article
Published: 09 November 2020 in The Plant Journal
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Tiller number is one of the most important agronomic traits that determine rice (Oryza sativa) yield. Active growth of tiller bud (TB) requires high amount of mineral nutrients, however, the mechanism underlying the distribution of mineral nutrients to TB with low transpiration is unknown. Here, we found that the distribution of zinc (Zn) to TB is mediated by OsZIP4; one of the ZIP (ZRT, IRT‐like protein) family member. The expression of OsZIP4 was highly detected in TB and nodes, and was induced by Zn deficiency. Immunostaining analysis revealed that OsZIP4 was mainly expressed in phloem of diffuse vascular bundles (DVBs) in the nodes and the axillary meristem. Mutation of OsZIP4 did not affect the total Zn uptake, but altered Zn distribution; less Zn was delivered to TB and new leaf, but more Zn was retained in the basal stems at the vegetative growth stage. Bioimaging analysis showed that the mutant aberrantly accumulated Zn in enlarged and transit vascular bundles of the basal node, whereas in wild type high accumulation of Zn was observed in the meristem part. At the reproductive stage, mutation of OsZIP4 resulted in delayed panicles development, which is associated with decreased Zn distribution to the panicles. Collectively, OsZIP4 is involved in transporting Zn to the phloem of DVBs in the nodes for subsequent distribution to TBs and other developing tissues. It also plays a role in transporting Zn to meristem cells in the TBs.

ACS Style

Shuai Mu; Naoki Yamaji; Akimasa Sasaki; Le Luo; Binbin Du; Jing Che; Huichao Shi; Haoqiang Zhao; Sheng Huang; Fenglin Deng; Zhenguo Shen; Mary Lou Guerinot; Luqing Zheng; Jian Feng Ma. A transporter for delivering zinc to the developing tiller bud and panicle in rice. The Plant Journal 2020, 105, 786 -799.

AMA Style

Shuai Mu, Naoki Yamaji, Akimasa Sasaki, Le Luo, Binbin Du, Jing Che, Huichao Shi, Haoqiang Zhao, Sheng Huang, Fenglin Deng, Zhenguo Shen, Mary Lou Guerinot, Luqing Zheng, Jian Feng Ma. A transporter for delivering zinc to the developing tiller bud and panicle in rice. The Plant Journal. 2020; 105 (3):786-799.

Chicago/Turabian Style

Shuai Mu; Naoki Yamaji; Akimasa Sasaki; Le Luo; Binbin Du; Jing Che; Huichao Shi; Haoqiang Zhao; Sheng Huang; Fenglin Deng; Zhenguo Shen; Mary Lou Guerinot; Luqing Zheng; Jian Feng Ma. 2020. "A transporter for delivering zinc to the developing tiller bud and panicle in rice." The Plant Journal 105, no. 3: 786-799.

Journal article
Published: 25 August 2020 in Plants
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Iron (Fe) homeostasis is essential for plant growth and development, and it is strictly regulated by a group of transcriptional factors. Iron-related transcription factor 3 (OsIRO3) was previously identified as a negative regulator for Fe deficiency response in rice. However, the molecular mechanisms by which OsIRO3 regulate Fe homeostasis is unclear. Here, we report that OsIRO3 is essential for responding to Fe deficiency and maintaining Fe homeostasis in rice. OsIRO3 is expressed in the roots, leaves, and base nodes, with a higher level in leaf blades at the vegetative growth stage. Knockout of OsIRO3 resulted in a hypersensitivity to Fe deficiency, with severe necrosis on young leaves and defective root development. The iro3 mutants accumulated higher levels of Fe in the shoot under Fe-deficient conditions, associated with upregulating the expression of OsNAS3, which lead to increased accumulation of nicotianamine (NA) in the roots. Further analysis indicated that OsIRO3 can directly bind to the E-box in the promoter of OsNAS3. Moreover, the expression of typical Fe-related genes was significantly up-regulated in iro3 mutants under Fe-sufficient conditions. Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the OsNAS3 expression.

ACS Style

Wujian Wang; Jun Ye; Yanran Ma; Ting Wang; Huixia Shou; Luqing Zheng. OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice. Plants 2020, 9, 1095 .

AMA Style

Wujian Wang, Jun Ye, Yanran Ma, Ting Wang, Huixia Shou, Luqing Zheng. OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice. Plants. 2020; 9 (9):1095.

Chicago/Turabian Style

Wujian Wang; Jun Ye; Yanran Ma; Ting Wang; Huixia Shou; Luqing Zheng. 2020. "OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice." Plants 9, no. 9: 1095.

Journal article
Published: 09 November 2018 in International Journal of Molecular Sciences
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The uptake and transport of iron (Fe) in plants are both important for plant growth and human health. However, little is known about the mechanism of Fe transport in plants, especially for crops. In the present study, the function of yellow stripe-like 13 (YSL13) in rice was analyzed. OsYSL13 was highly expressed in leaves, especially in leaf blades, whereas its expression was induced by Fe deficiency both in roots and shoots. Furthermore, the expression level of OsYSL13 was higher in older leaves than that in younger leaves. OsYSL13 was located in the plasma membrane. Metal measurement revealed that Fe concentrations were lower in the youngest leaf and higher in the older leaves of the osysl13 mutant under both Fe sufficiency and deficiency conditions, compared with the wild type and two complementation lines. Moreover, the Fe concentrations in the brown rice and seeds of the osysl13 mutant were also reduced. Opposite results were found in OsYSL13 overexpression lines. These results suggest that OsYSL13 is involved in Fe distribution in rice.

ACS Style

Chang Zhang; Kamran Iqbal Shinwari; Le Luo; Luqing Zheng. OsYSL13 Is Involved in Iron Distribution in Rice. International Journal of Molecular Sciences 2018, 19, 3537 .

AMA Style

Chang Zhang, Kamran Iqbal Shinwari, Le Luo, Luqing Zheng. OsYSL13 Is Involved in Iron Distribution in Rice. International Journal of Molecular Sciences. 2018; 19 (11):3537.

Chicago/Turabian Style

Chang Zhang; Kamran Iqbal Shinwari; Le Luo; Luqing Zheng. 2018. "OsYSL13 Is Involved in Iron Distribution in Rice." International Journal of Molecular Sciences 19, no. 11: 3537.

Journal article
Published: 25 September 2018 in The Plant Cell
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Alternative splicing (AS) of pre-mRNAs promotes transcriptome and proteome diversity and plays important roles in a wide range of biological processes. However, the role of AS in maintaining mineral nutrient homeostasis in plants are largely unknown. To clarify this role, we obtained whole transcriptome RNA sequencing data from rice (Oryza sativa) roots grown in the presence or absence of several mineral nutrients (Fe, Zn, Cu, Mn, and P). Our systematic analysis revealed 13,291 alternatively spliced genes, representing ~53.3% of the multiexon genes in the rice genome. As the overlap between differentially expressed genes and differentially alternatively spliced genes is small, a molecular understanding of the plant9s response to mineral deficiency is limited by analyzing differentially expressed genes alone. We found that the targets of AS are highly nutrient-specific. To verify the role of AS in mineral nutrition, we characterized mutants in genes encoding Ser/Arg (SR) proteins that function in AS. We identified several SR proteins as critical regulators of Zn, Mn, and P nutrition and showed that three SR protein-encoding genes regulate P uptake and remobilization between leaves and shoots of rice, demonstrating that AS has a key role in regulating mineral nutrient homeostasis in rice.-

ACS Style

Chunlan Dong; Fei He; Oliver Berkowitz; Jingxian Liu; Pengfei Cao; Min Tang; Huichao Shi; Wujian Wang; Qiaolu Li; Zhenguo Shen; James Whelan; Luqing Zheng. Alternative Splicing Plays a Critical Role in Maintaining Mineral Nutrient Homeostasis in Rice (Oryza sativa). The Plant Cell 2018, 30, 2267 -2285.

AMA Style

Chunlan Dong, Fei He, Oliver Berkowitz, Jingxian Liu, Pengfei Cao, Min Tang, Huichao Shi, Wujian Wang, Qiaolu Li, Zhenguo Shen, James Whelan, Luqing Zheng. Alternative Splicing Plays a Critical Role in Maintaining Mineral Nutrient Homeostasis in Rice (Oryza sativa). The Plant Cell. 2018; 30 (10):2267-2285.

Chicago/Turabian Style

Chunlan Dong; Fei He; Oliver Berkowitz; Jingxian Liu; Pengfei Cao; Min Tang; Huichao Shi; Wujian Wang; Qiaolu Li; Zhenguo Shen; James Whelan; Luqing Zheng. 2018. "Alternative Splicing Plays a Critical Role in Maintaining Mineral Nutrient Homeostasis in Rice (Oryza sativa)." The Plant Cell 30, no. 10: 2267-2285.

Journal article
Published: 01 September 2018 in Environmental and Experimental Botany
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Methionine sulfoxide reductases (MSRs), a family of enzymes catalyzing the conversion of methionine from its oxidized form into its reduced form, has a pivotal role in responding to oxidative stress. In the present study, we found that OsMSRB5 plays a crucial role in rice adaption to Cu stress. OsMSRB5 was mainly expressed in leaves, with low transcriptional levels of OsMSRB5 observed in seeds, stems, and roots; this gene was also induced by the excess copper (Cu) treatment. The functions of OsMSRB5 were analyzed through ectopic expression in Escherichia coli and functional disruption in rice. An in vitro enzymatic activity assay showed that OsMSRB5 had the ability to reduce free methionine-R-sulfoxide (Met-R-SO) and protein-bound-like Met-SO (dabsyl-Met-SO) to Met and dabsyl-Met, respectively. Ectopic expression of OsMSRB5 in E. coli conferred increased tolerance to excess Cu and methyl viologen (MV). Furthermore, OsMSRB5 mutation decreased Cu and MV tolerance in rice. Our results demonstrate that rice OsMSRB5 is a functional methionine sulfoxide reductase and is involved in defense against copper toxicity. In addition, rice seedlings responding to MV-induced oxidative stress showed a similar phenotype with excess Cu.

ACS Style

Tengwei Xiao; Mengmeng Mi; Changyong Wang; Meng Qian; Yahua Chen; Luqing Zheng; Hongsheng Zhang; Zhubing Hu; Zhenguo Shen; Yan Xia. A methionine-R-sulfoxide reductase, OsMSRB5, is required for rice defense against copper toxicity. Environmental and Experimental Botany 2018, 153, 45 -53.

AMA Style

Tengwei Xiao, Mengmeng Mi, Changyong Wang, Meng Qian, Yahua Chen, Luqing Zheng, Hongsheng Zhang, Zhubing Hu, Zhenguo Shen, Yan Xia. A methionine-R-sulfoxide reductase, OsMSRB5, is required for rice defense against copper toxicity. Environmental and Experimental Botany. 2018; 153 ():45-53.

Chicago/Turabian Style

Tengwei Xiao; Mengmeng Mi; Changyong Wang; Meng Qian; Yahua Chen; Luqing Zheng; Hongsheng Zhang; Zhubing Hu; Zhenguo Shen; Yan Xia. 2018. "A methionine-R-sulfoxide reductase, OsMSRB5, is required for rice defense against copper toxicity." Environmental and Experimental Botany 153, no. : 45-53.

Journal article
Published: 25 June 2018 in Plant and Cell Physiology
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Deficiency of copper (Cu) causes low fertility in many plant species, but the molecular mechanisms underlying distribution of Cu to the floral organs are poorly understood. Here, we found that a member of yellow-stripe like (YSL) family, YSL16 encoding the Cu-nicotianamine (Cu- NA) transporter, was highly expressed in the rachilla, with less expression in the palea and lemma of rice (Oryza sativa). GUS staining of transgenic rice carrying OsYSL16 promoter-GUS showed that OsYSL16 was mainly expressed in vascular bundles of rachilla as well as palea and lemma. Knockout of OsYSL16 resulted in decreased Cu distribution to the stamens, but increased distribution to the palea and lemma. A short-term (24 h) 65Cu-labelling experiment confirmed increased Cu concentration of palea and lemma in the mutant. Furthermore, we found that redistribution of Cu from palea and lemma was impaired in osysl16 mutant after exposure to Cu-free solution. osysl16 mutant showed low pollen germination, but this was rescued by addition of Cu in the medium. Our results indicate that OsYSL16 expressed in the vascular bundles of rachilla is important for preferential distribution of Cu to the stamens, while OsYSL16 in vascular bundles of palea and lemma is involved in Cu redistribution under Cu-limited conditions in rice.

ACS Style

Chang Zhang; Wenhui Lu; Yang Yang; Zhenguo Shen; Jian Feng Ma; Luqing Zheng. OsYSL16 is Required for Preferential Cu Distribution to Floral Organs in Rice. Plant and Cell Physiology 2018, 59, 2039 -2051.

AMA Style

Chang Zhang, Wenhui Lu, Yang Yang, Zhenguo Shen, Jian Feng Ma, Luqing Zheng. OsYSL16 is Required for Preferential Cu Distribution to Floral Organs in Rice. Plant and Cell Physiology. 2018; 59 (10):2039-2051.

Chicago/Turabian Style

Chang Zhang; Wenhui Lu; Yang Yang; Zhenguo Shen; Jian Feng Ma; Luqing Zheng. 2018. "OsYSL16 is Required for Preferential Cu Distribution to Floral Organs in Rice." Plant and Cell Physiology 59, no. 10: 2039-2051.

Review
Published: 24 January 2018 in International Journal of Molecular Sciences
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Lignin is one of the main components of plant cell wall and it is a natural phenolic polymer with high molecular weight, complex composition and structure. Lignin biosynthesis extensively contributes to plant growth, tissue/organ development, lodging resistance and the responses to a variety of biotic and abiotic stresses. In the present review, we systematically introduce the biosynthesis of lignin and its regulation by genetic modification and summarize the main biological functions of lignin in plants and their applications. We hope this review will give an in-depth understanding of the important roles of lignin biosynthesis in various plants’ biological processes and provide a theoretical basis for the genetic improvement of lignin content and composition in energy plants and crops.

ACS Style

Qingquan Liu; Le Luo; Luqing Zheng. Lignins: Biosynthesis and Biological Functions in Plants. International Journal of Molecular Sciences 2018, 19, 335 .

AMA Style

Qingquan Liu, Le Luo, Luqing Zheng. Lignins: Biosynthesis and Biological Functions in Plants. International Journal of Molecular Sciences. 2018; 19 (2):335.

Chicago/Turabian Style

Qingquan Liu; Le Luo; Luqing Zheng. 2018. "Lignins: Biosynthesis and Biological Functions in Plants." International Journal of Molecular Sciences 19, no. 2: 335.

Regular article
Published: 01 December 2017 in Plant and Soil
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Cadmium (Cd) is one of the most toxic heavy metals. Cd tolerance ability differs among varieties in plants, but the underlying molecular mechanisms remain largely unknown. In this study, we identified genes that are involved in Cd stress responses and different Cd tolerances of two V. sativa varieties (Cd-tolerant variety (L3) and Cd-sensitive variety (ZM)). Transcriptomic analysis using Illumina pair-end sequencing was carried out on root tissues of L3 and ZM grown with 5 μM and 50 μM of Cd treatments. A de novo assembled V. sativa transcriptome was generated. Differentially expressed genes (DEGs) were assigned to Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and enrichment analysis was performed. The expression of selected DEGs were confirmed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR). A total of 49,062 sequences were identified as unigenes. In the 5 μM Cd treatment, 69 and 28 differentially expressed unigenes were found as compared with the control in the L3 and ZM respectively, while in the 50 μM Cd treatment, 1036 and 335 differentially expressed unigenes were found in comparison with the control in L3 and ZM. Pathway enrichment analysis suggested that genes related to the cell wall, stress response, the glutathione pathway, metal transporters, and transcription factors are commonly up-regulated in response to Cd stress in both varieties. However, the expression of metal transporter genes and transcription factor genes showed significant differential responses to Cd stress. In addition to the regulation of transcription by transcription factors, metal transporters play a vital role in controlling the different Cd tolerances of V. sativa varieties L3 and ZM.

ACS Style

Haiyun Rui; Xingxing Zhang; Kamran Shinwari; Luqing Zheng; Zhenguo Shen. Comparative transcriptomic analysis of two Vicia sativa L. varieties with contrasting responses to cadmium stress reveals the important role of metal transporters in cadmium tolerance. Plant and Soil 2017, 423, 241 -255.

AMA Style

Haiyun Rui, Xingxing Zhang, Kamran Shinwari, Luqing Zheng, Zhenguo Shen. Comparative transcriptomic analysis of two Vicia sativa L. varieties with contrasting responses to cadmium stress reveals the important role of metal transporters in cadmium tolerance. Plant and Soil. 2017; 423 (1-2):241-255.

Chicago/Turabian Style

Haiyun Rui; Xingxing Zhang; Kamran Shinwari; Luqing Zheng; Zhenguo Shen. 2017. "Comparative transcriptomic analysis of two Vicia sativa L. varieties with contrasting responses to cadmium stress reveals the important role of metal transporters in cadmium tolerance." Plant and Soil 423, no. 1-2: 241-255.

Journal article
Published: 01 November 2017 in Plant Science
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Cadmium (Cd) is one of the most toxic heavy metal elements in nature, and it causes serious damage to plant cells. Here, we report that a transcription factor OsMYB45 is involved in Cd stress response in rice. OsMYB45 is highly expressed in rice leaves, husks, stamens, pistils, and lateral roots, and its expression is induced by Cd stress. OsMYB45 fused to green fluorescent protein localized to the cell nucleus in onion epidermal cells. Mutation of OsMYB45 resulted in hypersensitivity to Cd treatment, and the concentration of H2O2 in the leaves of mutant nearly doubled, while catalase (CAT) activity was halved compared with the wild-type. Moreover, gene expression analysis indicated that OsCATA and OsCATC expression is significantly lower in the mutant than in the wild-type. In addition, overexpression of OsMYB45 in the mutant complemented the mutant phenotype. Taken together, OsMYB45 plays an important role in tolerance to Cd stress in rice.

ACS Style

Shubao Hu; Yao Yu; Qiuhong Chen; Guangmao Mu; Zhenguo Shen; Luqing Zheng. OsMYB45 plays an important role in rice resistance to cadmium stress. Plant Science 2017, 264, 1 -8.

AMA Style

Shubao Hu, Yao Yu, Qiuhong Chen, Guangmao Mu, Zhenguo Shen, Luqing Zheng. OsMYB45 plays an important role in rice resistance to cadmium stress. Plant Science. 2017; 264 ():1-8.

Chicago/Turabian Style

Shubao Hu; Yao Yu; Qiuhong Chen; Guangmao Mu; Zhenguo Shen; Luqing Zheng. 2017. "OsMYB45 plays an important role in rice resistance to cadmium stress." Plant Science 264, no. : 1-8.

Journals
Published: 29 June 2017 in Metallomics
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Correction for ‘Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae’ by Xishi Zhou et al., Metallomics, 2017, DOI: 10.1039/c7mt00072c.

ACS Style

Xishi Zhou; Lei Fu; Yan Xia; Luqing Zheng; Chen Chen; Zhenguo Shen; Yahua Chen. Correction: Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae. Metallomics 2017, 9, 989 -989.

AMA Style

Xishi Zhou, Lei Fu, Yan Xia, Luqing Zheng, Chen Chen, Zhenguo Shen, Yahua Chen. Correction: Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae. Metallomics. 2017; 9 (7):989-989.

Chicago/Turabian Style

Xishi Zhou; Lei Fu; Yan Xia; Luqing Zheng; Chen Chen; Zhenguo Shen; Yahua Chen. 2017. "Correction: Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae." Metallomics 9, no. 7: 989-989.

Journals
Published: 06 June 2017 in Metallomics
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The ultrastructure of transverse sections of root tips ofT. patulawith and without AMF inoculation and Cu content determined by energy spectrum analysis.

ACS Style

Xishi Zhou; Lei Fu; Yan Xia; Luqing Zheng; Chen Chen; Zhenguo Shen; Yahua Chen. Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae. Metallomics 2017, 9, 936 -948.

AMA Style

Xishi Zhou, Lei Fu, Yan Xia, Luqing Zheng, Chen Chen, Zhenguo Shen, Yahua Chen. Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae. Metallomics. 2017; 9 (7):936-948.

Chicago/Turabian Style

Xishi Zhou; Lei Fu; Yan Xia; Luqing Zheng; Chen Chen; Zhenguo Shen; Yahua Chen. 2017. "Arbuscular mycorrhizal fungi enhance the copper tolerance of Tagetes patula through the sorption and barrier mechanisms of intraradical hyphae." Metallomics 9, no. 7: 936-948.

Journal article
Published: 07 April 2017 in International Journal of Environmental Research and Public Health
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With the rapid progress of industrialization, the effects of environmental contamination on plant toxicity, and subsequently on human health, is a growing concern. For example, the heavy metal pollution of soil such as that caused by cadmium (Cd) is a serious threat. Therefore, screening for pollution-safe edible plants is an essential approach for growing plants under heavy metal-contaminated soils. In the current study, 35 Chinese cabbage (Brassica pekinensis L.) cultivars were selected with the aim of screening for Cd-safe cultivars (CSCs), analyzing their safety, and exploring the mechanism of Cd accumulation. Our field-culture experiments revealed that the Cd content in the edible parts of the cultivars were varied and were determined to possibly be CSCs. Hydroponics experiments were used to simulate six different degrees of soil contamination (high and low Cd concentrations) on possible CSCs. The results indicated a significant difference (p < 0.05) in Cd concentration in the cultivars, and verified the safety of these possible CSCs. The analyses of the transport coefficient and expression levels showed that the differences in Cd accumulation among the Chinese cabbage cultivars were related to the expression of genes involved in absorption and transport rather than a root-to-shoot translocation limitation.

ACS Style

Jingjie Wang; Nan Yu; Guangmao Mu; Kamran Iqbal Shinwari; Zhenguo Shen; Luqing Zheng. Screening for Cd-Safe Cultivars of Chinese Cabbage and a Preliminary Study on the Mechanisms of Cd Accumulation. International Journal of Environmental Research and Public Health 2017, 14, 395 .

AMA Style

Jingjie Wang, Nan Yu, Guangmao Mu, Kamran Iqbal Shinwari, Zhenguo Shen, Luqing Zheng. Screening for Cd-Safe Cultivars of Chinese Cabbage and a Preliminary Study on the Mechanisms of Cd Accumulation. International Journal of Environmental Research and Public Health. 2017; 14 (4):395.

Chicago/Turabian Style

Jingjie Wang; Nan Yu; Guangmao Mu; Kamran Iqbal Shinwari; Zhenguo Shen; Luqing Zheng. 2017. "Screening for Cd-Safe Cultivars of Chinese Cabbage and a Preliminary Study on the Mechanisms of Cd Accumulation." International Journal of Environmental Research and Public Health 14, no. 4: 395.

Journal article
Published: 30 January 2017 in International Journal of Molecular Sciences
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Laccases are encoded by a multigene family and widely distributed in plant genomes where they play roles oxidizing monolignols to produce higher-order lignin involved in plant development and stress responses. We identified 30 laccase genes (OsLACs) from rice, which can be divided into five subfamilies, mostly expressed during early development of the endosperm, growing roots, and stems. OsLACs can be induced by hormones, salt, drought, and heavy metals stresses. The expression level of OsLAC10 increased 1200-fold after treatment with 20 μM Cu for 12 h. The laccase activities of OsLAC10 were confirmed in an Escherichia coli expression system. Lignin accumulation increased in the roots of Arabidopsis over-expressing OsLAC10 (OsLAC10-OX) compared to wild-type controls. After growth on 1/2 Murashige and Skoog (MS) medium containing toxic levels of Cu for seven days, roots of the OsLAC10-OX lines were significantly longer than those of the wild type. Compared to control plants, the Cu concentration decreased significantly in roots of the OsLAC10-OX line under hydroponic conditions. These results provided insights into the evolutionary expansion and functional divergence of OsLAC family. In addition, OsLAC10 is likely involved in lignin biosynthesis, and reduces the uptake of Cu into roots required for Arabidopsis to develop tolerance to Cu.

ACS Style

Qingquan Liu; Le Luo; Xiaoxiao Wang; Zhenguo Shen; Luqing Zheng. Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis. International Journal of Molecular Sciences 2017, 18, 209 .

AMA Style

Qingquan Liu, Le Luo, Xiaoxiao Wang, Zhenguo Shen, Luqing Zheng. Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis. International Journal of Molecular Sciences. 2017; 18 (2):209.

Chicago/Turabian Style

Qingquan Liu; Le Luo; Xiaoxiao Wang; Zhenguo Shen; Luqing Zheng. 2017. "Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis." International Journal of Molecular Sciences 18, no. 2: 209.

Journal article
Published: 01 August 2015 in Journal of Hazardous Materials
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Metallothioneins (MTs) are low-molecular-weight, cysteine-rich metal-binding proteins found in numerous genera and species, but their functions in abiotic stress tolerance remain unclear. Here, a MT gene from Oryza sativa, OsMT2c, was isolated and characterized, encoding a type 2 MT, and observed expression in the roots, leaf sheathes, and leaves, but only weak expression in seeds. OsMT2c was upregulated by copper (Cu) and hydrogen peroxide (H2O2) treatments. Excessive Cu elicited a rapid and sustained production and release of H2O2 in rice, and exogenous H2O2 scavengers N,N'-dimethylthiourea (DMTU) and ascorbic acid (Asc) decreased H2O2 production and OsMT2c expression. Furthermore, the expression of OsMT2c increased in the osapx2 mutant in which the H2O2 levels were higher than in wild-type (WT) plants. These results showed that Cu increased MT2c expression through the production and accumulation of Cu-induced H2O2 in O. sativa. In addition, the transgenic OsMT2c-overexpressing Arabidopsis displayed improved tolerance to Cu stress and exhibited increased reactive oxygen species (ROS) scavenging ability compared to WT and empty-vector (Ev) seedlings.

ACS Style

Jia Liu; Xiaoting Shi; Meng Qian; Luqing Zheng; Chunlan Lian; Yan Xia; Zhenguo Shen. Copper-induced hydrogen peroxide upregulation of a metallothionein gene, OsMT2c, from Oryza sativa L. confers copper tolerance in Arabidopsis thaliana. Journal of Hazardous Materials 2015, 294, 99 -108.

AMA Style

Jia Liu, Xiaoting Shi, Meng Qian, Luqing Zheng, Chunlan Lian, Yan Xia, Zhenguo Shen. Copper-induced hydrogen peroxide upregulation of a metallothionein gene, OsMT2c, from Oryza sativa L. confers copper tolerance in Arabidopsis thaliana. Journal of Hazardous Materials. 2015; 294 ():99-108.

Chicago/Turabian Style

Jia Liu; Xiaoting Shi; Meng Qian; Luqing Zheng; Chunlan Lian; Yan Xia; Zhenguo Shen. 2015. "Copper-induced hydrogen peroxide upregulation of a metallothionein gene, OsMT2c, from Oryza sativa L. confers copper tolerance in Arabidopsis thaliana." Journal of Hazardous Materials 294, no. : 99-108.

Journal article
Published: 19 October 2014 in Plant and Soil
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Induction of lignin biosynthesis is an adaptive response of plants subjected to many abiotic stresses. In this study, we examined the response of lignin biosynthesis to copper (Cu) stress, with a particular focus on the regulatory mechanism. We performed a transcriptomic analysis of rice (Oryza sativa L.) roots, and the microarray data on lignin biosynthesis pathway genes were corroborated by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analysis. Physiological analyses of rice seedlings treated with Cu(II) sulfate (CuSO4) were used to confirm the relationship between excess Cu and lignin biosynthesis. In addition, we examined the role of hydrogen peroxide (H2O2) in Cu-induced lignin biosynthesis through pretreatments with an NADPH oxidase inhibitor (diphenyleneiodonium, DPI) and a H2O2 scavenger (dimethylthiourea, DMTU). Lignin biosynthesis pathway genes were upregulated under Cu stress. The lignin content of rice roots increased significantly with increasing concentrations and durations of Cu treatment; elevations in root lignin content were correlated with marked inhibitions in root growth. Pretreatments with DPI and DMTU inhibited the activities of Cu-induced lignin polymerization enzymes (peroxidase, POD and laccase, LAC) and lignin accumulation in rice roots. Conversely, exogenous H2O2 increased the root lignin content. Rice roots under Cu stress accumulate lignin through enhanced polymerization of lignin monolignol, a mechanism that requires Cu stress induced H2O2.

ACS Style

Qingquan Liu; Li Zheng; Fei He; Fang-Jie Zhao; Zhenguo Shen; Luqing Zheng. Transcriptional and physiological analyses identify a regulatory role for hydrogen peroxide in the lignin biosynthesis of copper-stressed rice roots. Plant and Soil 2014, 387, 323 -336.

AMA Style

Qingquan Liu, Li Zheng, Fei He, Fang-Jie Zhao, Zhenguo Shen, Luqing Zheng. Transcriptional and physiological analyses identify a regulatory role for hydrogen peroxide in the lignin biosynthesis of copper-stressed rice roots. Plant and Soil. 2014; 387 (1-2):323-336.

Chicago/Turabian Style

Qingquan Liu; Li Zheng; Fei He; Fang-Jie Zhao; Zhenguo Shen; Luqing Zheng. 2014. "Transcriptional and physiological analyses identify a regulatory role for hydrogen peroxide in the lignin biosynthesis of copper-stressed rice roots." Plant and Soil 387, no. 1-2: 323-336.

Journal article
Published: 31 July 2014 in Plant and Soil
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To examine heavy metal-induced regulatory mechanisms at the transcriptional level, a cell wall-associated receptor kinase (WAK) gene, OsWAK11 and its upstream promoter region (−946/+28) were isolated from Oryza sativa. OsWAK11 expression in response to abiotic stress was examined using a β-glucuronidase (GUS) gene fusion. Semi-quantitative RT-PCR was used to analyze expression of the OsWAK11 gene. Histochemical detection of GUS was conducted by X-gluc staining methods, and fluorometric measurements of GUS activity were made with 4-methyl umbelliferyl glucuronide (MUG) substrate. The WAK promoter (−946/+28) responded to aluminum chloride, sodium chloride, and copper (II) sulfate with 3.0-, 2.2-, or 6.4-fold induction of GUS activity, respectively. Sodium nitroprusside and wounding treatment stimulated GUS activity. A histochemical analysis revealed strong GUS staining in the hypocotyls, cotyledons, first leaf, and petiole of cotyledons in transgenic tobacco seedlings. Strong GUS staining was also observed in the stigma and ovary of mature flowers, but not in the stamens. OsWAK11 expression is regulated by aluminum, sodium, and copper. The GUS expression observed in transgenic tobacco carrying WAK11 promoter demonstrated significant tissue-specificity. The OsWAK11 promoter was strongly upregulated in response to metals and wounding.

ACS Style

Wei Hu; Yanyan Lv; Wenrui Lei; Xiang Li; Yahua Chen; Luqing Zheng; Yan Xia; Zhenguo Shen. Cloning and characterization of the Oryza sativa wall-associated kinase gene OsWAK11 and its transcriptional response to abiotic stresses. Plant and Soil 2014, 384, 335 -346.

AMA Style

Wei Hu, Yanyan Lv, Wenrui Lei, Xiang Li, Yahua Chen, Luqing Zheng, Yan Xia, Zhenguo Shen. Cloning and characterization of the Oryza sativa wall-associated kinase gene OsWAK11 and its transcriptional response to abiotic stresses. Plant and Soil. 2014; 384 (1-2):335-346.

Chicago/Turabian Style

Wei Hu; Yanyan Lv; Wenrui Lei; Xiang Li; Yahua Chen; Luqing Zheng; Yan Xia; Zhenguo Shen. 2014. "Cloning and characterization of the Oryza sativa wall-associated kinase gene OsWAK11 and its transcriptional response to abiotic stresses." Plant and Soil 384, no. 1-2: 335-346.

Journal article
Published: 26 November 2010 in Journal of Experimental Botany
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Plants employ two distinct strategies to obtain iron (Fe) from the soil. In Strategy I but not Strategy II plants, Fe limitation invokes ethylene production which regulates Fe deficiency responses. Oryza sativa (rice) is the only graminaceous plant described that possesses a Strategy I-like system for iron uptake as well as the classic Strategy II system. Ethylene production of rice roots was significantly increased when grown under Fe-depleted conditions. Moreover, 1-aminocyclopropane-1-carboxylic acid (ACC) treatment, a precursor of ethylene, conferred tolerance to Fe deficiency in rice by increasing internal Fe availability. Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems. RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1. By contrast, in Hordeum vulgare L. (barley), no ethylene production or ethylene-mediated effects of Fe response could be detected. In conclusion, Fe-limiting conditions increased ethylene production and signalling in rice, which is novel in Strategy II plant species.

ACS Style

Jiaojiao Wu; Chuang Wang; Luqing Zheng; Lu Wang; Yunlong Chen; James Whelan; Huixia Shou. Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa. Journal of Experimental Botany 2010, 62, 667 -674.

AMA Style

Jiaojiao Wu, Chuang Wang, Luqing Zheng, Lu Wang, Yunlong Chen, James Whelan, Huixia Shou. Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa. Journal of Experimental Botany. 2010; 62 (2):667-674.

Chicago/Turabian Style

Jiaojiao Wu; Chuang Wang; Luqing Zheng; Lu Wang; Yunlong Chen; James Whelan; Huixia Shou. 2010. "Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa." Journal of Experimental Botany 62, no. 2: 667-674.

Journal article
Published: 11 November 2010 in Journal of Plant Biology
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Drought and salinity are major abiotic stresses affecting rice production. To improve plant tolerance to salinity and drought, we overexpressed rice Na+/H+ exchangers (OsNHX1) and H+-pyrophosphatase in tonoplasts (OsVP1) in a japonica elite rice cultivar, Zhonghua 11. Compared with our wild-type control, transgenic plants overexpressing both genes incurred less damage when exposed to long-term treatment with 100 mM NaCl or water deprivation. Under high-saline conditions, the transformants accumulated less Na+ and malondialdehyde in the leaves, thereby allowing the plants to maintain a low level of leaf water potential and reduce stress-induced damage. Those transgenics also had higher photosynthetic activity during the stress period. Under those conditions, they also showed an increase in root biomass, which enabled more water uptake. These results suggest that OsVP1 and OsNHX1 improve the tolerance of rice crops against drought and salt by employing multiple strategies in addition to osmotic regulation.

ACS Style

Shiping Liu; Luqing Zheng; Yanhong Xue; Qian Zhang; Lu Wang; Huixia Shou. Overexpression of OsVP1 and OsNHX1 Increases Tolerance to Drought and Salinity in Rice. Journal of Plant Biology 2010, 53, 444 -452.

AMA Style

Shiping Liu, Luqing Zheng, Yanhong Xue, Qian Zhang, Lu Wang, Huixia Shou. Overexpression of OsVP1 and OsNHX1 Increases Tolerance to Drought and Salinity in Rice. Journal of Plant Biology. 2010; 53 (6):444-452.

Chicago/Turabian Style

Shiping Liu; Luqing Zheng; Yanhong Xue; Qian Zhang; Lu Wang; Huixia Shou. 2010. "Overexpression of OsVP1 and OsNHX1 Increases Tolerance to Drought and Salinity in Rice." Journal of Plant Biology 53, no. 6: 444-452.

Research article
Published: 16 April 2010 in PLOS ONE
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Polished rice is a staple food for over 50% of the world's population, but contains little bioavailable iron (Fe) to meet human needs. Thus, biofortifying the rice grain with novel promoters or enhancers of Fe utilization would be one of the most effective strategies to prevent the high prevalence of Fe deficiency and iron deficiency anemia in the developing world. We transformed an elite rice line cultivated in Southern China with the rice nicotianamine synthase gene (OsNAS1) fused to a rice glutelin promoter. Endosperm overexpression of OsNAS1 resulted in a significant increase in nicotianamine (NA) concentrations in both unpolished and polished grain. Bioavailability of Fe from the high NA grain, as measured by ferritin synthesis in an in vitro Caco-2 cell model that simulates the human digestive system, was twice as much as that of the control line. When added at 1∶1 molar ratio to ferrous Fe in the cell system, NA was twice as effective when compared to ascorbic acid (one of the most potent known enhancers of Fe bioavailability) in promoting more ferritin synthesis. Our data demonstrated that NA is a novel and effective promoter of iron utilization. Biofortifying polished rice with this compound has great potential in combating global human iron deficiency in people dependent on rice for their sustenance.

ACS Style

Luqing Zheng; Zhiqiang Cheng; Chunxiang Ai; Xinhang Jiang; Xiaoshu Bei; Ye Zheng; Raymond P. Glahn; Ross M. Welch; Dennis D. Miller; Xin Gen Lei; Huixia Shou. Nicotianamine, a Novel Enhancer of Rice Iron Bioavailability to Humans. PLOS ONE 2010, 5, e10190 -e10190.

AMA Style

Luqing Zheng, Zhiqiang Cheng, Chunxiang Ai, Xinhang Jiang, Xiaoshu Bei, Ye Zheng, Raymond P. Glahn, Ross M. Welch, Dennis D. Miller, Xin Gen Lei, Huixia Shou. Nicotianamine, a Novel Enhancer of Rice Iron Bioavailability to Humans. PLOS ONE. 2010; 5 (4):e10190-e10190.

Chicago/Turabian Style

Luqing Zheng; Zhiqiang Cheng; Chunxiang Ai; Xinhang Jiang; Xiaoshu Bei; Ye Zheng; Raymond P. Glahn; Ross M. Welch; Dennis D. Miller; Xin Gen Lei; Huixia Shou. 2010. "Nicotianamine, a Novel Enhancer of Rice Iron Bioavailability to Humans." PLOS ONE 5, no. 4: e10190-e10190.