Because of the high cost and safety of ultra-high magnetic resonance imaging (MRI), its application has certain limitations. Whereas 0.5–3 T MRI has been widely applied in hospitals, static magnetic fields (SMFs) have been shown to improve mice mental health and have anti-tumor potentials. Here, we compared the effects of the upward and downward 150 mT SMF groups with the sham group on C57BL/6J adult female mice. Locomotor and exploratory activity were also measured by behavioral tests, including the open field and elevated plus test. Additionally, physiology, pathology indicators and gut microbiota were examined. We found that 150 mT SMFs long-term exposure enhanced locomotive and exploratory activity of mice, especially the downward 150 mT SMF. Compared with the downward 150 mT SMF group, the movement speed and distance in the center area of the sham group were increased by 65.99% (p < 0.0001) and 68.58% (p = 0.0038), respectively. Moreover, compared to the sham group, downward 150 mT SMF increased the number of entrances to the center area by 67.0% (p = 0.0082) and time in the center area by 77.12% (p = 0.0054). Additionally, we observed that upward 150 mT SMF improved the number of follicles (~2.5 times, p = 0.0325) and uterine glands through increasing the total antioxidant capacity and reducing lipid peroxidation level in mice. Gut microbiome analysis showed that 150 mT SMFs long-term exposure improved the microbiota abundance (Clostridium, Bifidobacterium, Ralstonia and Yaniella) in the genus level, which may affect metabolism, anxiety and behavior in adult female mice. Our results demonstrated that 150 mT SMFs long-term exposure not only had good biosafety, but also improved athletic performance, emotion and the function of ovarian, uterine and gut microbiota abundance in adult female mice, which unraveled the potential of moderate long-term SMF exposure in clinical applications.

Introduction: Clinodiside A is a natural bioactive component of Clinopodii herba, a genuine regional herb from Anhui Province (China) with multiple biological activities. This study aimed to explore the therapeutic potential of Clinodiside A in ulcerative colitis (UC). Methods: Dextran sulfate sodium (DSS) was used to establish UC models both in vivo and in vitro . The models were treated with Clinodiside A. Parameters including body weight, disease activity index (DAI) score, colon length, and inflammation were assessed. Iron overload, lipid peroxidation, and intestinal epithelial repair were also evaluated. In vitro experiments using HCT116 cells examined cell damage, motility, and recovery. Results: Clinodiside A alleviated DSS-induced UC consequences, including weight loss, increased DAI score, colon shortening, and inflammation. These effects were associated with reduced iron overload, reduced lipid peroxidation, and enhanced intestinal epithelial repair. In HCT116 cells, Clinodiside A ameliorated damage, enhanced motility, and promoted recovery of the intestinal epithelium. Discussion: Our research validates the therapeutic and protective effects of Clinodiside A from Clinopodii herba on UC and expands its application potential.