The exploration of antibiotic resistance genes (ARGs) in drinking water reservoirs is an emerging field. Using a curated database, we enhanced the ARG detection and conducted a comprehensive analysis using 2.2 Tb of deep metagenomic sequencing data to determine the distribution of ARGs across 16 drinking water reservoirs and associated environments. Our findings reveal a greater diversity of ARGs in sediments than in water, underscoring the importance of extensive background surveys. Crucial ARG carriers-specifically Acinetobacter, Pseudomonas, and Mycobacterium were identified in drinking water reservoirs. Extensive analysis of the data uncovered a considerable concern for drinking water safety, particularly in regions reliant on river sources. Mobile genetic elements have been found to contribute markedly to the propagation of ARGs. The results of this research suggest that the establishment of drinking water reservoirs for supplying raw water may be an effective strategy for alleviating the spread of water-mediated ARGs.

The development of ecological circular agriculture has been highly encouraged to recycle agricultural wastes, reduce mineral fertilizer input, and protect the environment. Biogas slurry (BS), a by-product of biogas production generated from anaerobic digestion of animal waste and crop residues, is often considered a substitute to reduce mineral fertilizer input. Being a cheap source of organic matter and plant nutrients, its application may improve soil fertility and yield quality and quantity. The field experiments were conducted in 2016 and 2017 to study the plant growth responses and forage quality by applying biogas slurry to replace chemical synthetic fertilizer (CSF). Results revealed that biogas slurry combination with chemical synthetic fertilizer significantly (p < 0.05) improved the growth of Italian ryegrass on treatment with T2, and the Italian ryegrass dry matter was increased by more than 9.00%, while the stem-to-leaf ratio was decreased by more than 12% (second cutting), in comparison with only chemical synthetic fertilizer group. In the case of forage quality, the crude protein (CP) and crude fiber (CF) content had a significant difference was observed between the T0 and T2 treatment group. Compare with the chemical synthetic fertilizer group, the CP content improved by 10.35%, and the CF content decreased about 10.00%. Based on these results, it was concluded that the application of 37.5 kg/ha CSF + 100.5 t/ha BS could improve the production of biomass and forage quality in Italian ryegrass.

Ionic liquid-based air-assisted liquid–liquid microextraction (IL-AALLME) combined with dispersive micro-solid phase extraction (D-μ-SPE) was applied for the extraction and preconcentration of Cu prior to analysis by graphite furnace atomic absorption spectrometry. IL [Hmim][PF₆] was used as the extractant, non-ionic surfactant Triton X-100 as the emulsifier and Fe₃O₄ nanoparticles as the sorbent. The emulsion was rapidly formed by pulling in and pushing out the mixture of aqueous sample solution and extraction solvent repeatedly using a 10 mL glass syringe. Then, the extractant phase was mixed with Fe₃O₄ magnetic nanoparticles and separated using a magnet. As a result, the conventional tedious procedure associated with AALLME such as centrifugation was eliminated and the analytical process was accelerated. The experimental parameters affecting the extraction efficiency of Cu such as pH, IL volume, Triton X-100 concentration, Fe₃O₄ amount, number of extraction times and ultrasound time were investigated and optimized. Under the optimal conditions, the calibration curve was linear in the range of 1.5–120 ng mL⁻¹ for Cu with a correlation coefficient (R) of 0.9992. The limit of detection was 0.5 ng mL⁻¹. Finally, the applicability of this newly developed method was investigated for the analysis of Cu in real water samples.

Drought is among the most important natural disasters with severe effects on animals and plants. MicroRNAs are a class of noncoding RNAs that play a crucial role in plant growth, development, and response to stress factors, including drought. However, the microRNAs in drought responses in common vetch (Vicia sativa), an annual herbaceous leguminous plant commonly used for forage by including it in mixed seeding during winter and spring, have not been characterized. To explore the microRNAs’ response to drought in common vetch, we sequenced 10 small RNA (sRNA) libraries by the next-generation sequencing technology. We obtained 379 known miRNAs belonging to 38 families and 47 novel miRNAs. The two groups had varying numbers of differentially expressed miRNAs: 85 in the comparison group D5 vs C5 and 38 in the comparison group D3 vs C3. Combined analysis of mRNA and miRNA in the same samples under drought treatment identified 318 different target genes of 123 miRNAs. Functional annotation of the target genes revealed that the miRNAs regulate drought-responsive genes, such as leucine-rich repeat receptor-like kinase-encoding genes (LRR-RLKs), ABC transporter G family member 1 (ABCG1), and MAG2-interacting protein 2 (MIP2). The genes were involved in various pathways, including cell wall biosynthesis, reactive oxygen removal, and protein transport. The findings in this study provide new insights into the miRNA-mediated regulatory networks of drought stress response in common vetch.

Light availability may have direct effects on reproduction through resource availability, and indirect effects on female reproduction by influencing plant-pollinator interactions. Floral display size, pollinator visitation per flower, resource and pollen limitation of fruit and seed production were quantified in a forested patch and an adjacent open patch of two populations of the perennial herb Hosta ventricosa. Plants in the open patch produced significantly larger floral displays than those in the forested patch in both populations. Floral display size had a positive effect on pollinator visitation rate per flower in one population, but no effect in the other. Plants in the open patch received approximately 8–11 times more visitation rates and produced significantly more fruit and seeds per flower than those in the forested patch. However, supplemental pollination resulted in significantly more fruit and seed production per flower compared to natural pollination in the forested patch but not in the open patch in one population, and did not enhance fruit and seed production in either the forested or the open patch in the other. In both populations, supplementally pollinated plants in the open patch produced significantly more fruit and seeds per flower than supplementally pollinated plants in the forested patch. In H. ventricosa, local variation in light conditions could affect pollinator activity and influence female reproduction through resource availability; however differences in the degree of pollen limitation between local habitats were found to be population-specific.