Aims: To test the relationship between practice environment and turnover intention, considering the moderation of perceived organizational support (POS) and the mediation of general well‐being (GWB) and occupational burnout (OB) among the nurse population.Design: A cross‐sectional survey design.Methods: Based on a sample of 474 nurses from two hospitals between May 2020 and September 2021, a moderated multi‐mediation model was tested in which GWB and OB served as two mediators between practice environments and nurses' turnover intention, and POS was regarded as a moderator between practice environments and GWB.Results: This current study supported a moderated multi‐mediation model where the relationship between practice environment and turnover intention (B = 0.33, p < 0.001) was mediated by GWB (B = 0.17, p < 0.001) and OB (B = 0.18, p < 0.001), and POS moderated the effect of practice environment and GWB (B = 0.19, p < 0.001).Public Contribution: The findings of this study can inform the design of effective organizational support and meet nurse emotional needs programmes to reduce the turnover intention among nurses.