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Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.
Shuming Liu; Hongmei Liu; Rui Chen; Yong Ma; Bo Yang; Zhiyong Chen; Yunshan Liang; Jun Fang; Yunhua Xiao. Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.). Plants 2021, 10, 912 .
AMA StyleShuming Liu, Hongmei Liu, Rui Chen, Yong Ma, Bo Yang, Zhiyong Chen, Yunshan Liang, Jun Fang, Yunhua Xiao. Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.). Plants. 2021; 10 (5):912.
Chicago/Turabian StyleShuming Liu; Hongmei Liu; Rui Chen; Yong Ma; Bo Yang; Zhiyong Chen; Yunshan Liang; Jun Fang; Yunhua Xiao. 2021. "Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)." Plants 10, no. 5: 912.
Background: Miscanthus, which is a leading dedicated-energy grass in Europe and in parts of Asia, is expected to play a key role in the development of the future bioeconomy. However, due to its complex genetic background, it is difficult to investigate phylogenetic relationships and the evolution of gene function in this genus. Here, we investigated 50 Miscanthus germplasms: 1 female parent (M. lutarioriparius), 30 candidate male parents (M. lutarioriparius, M. sinensis, and M. sacchariflorus), and 19 offspring. We used high-throughput Specific-Locus Amplified Fragment sequencing (SLAF-seq) to identify informative single nucleotide polymorphisms (SNPs) in all germplasms.Results: We identified 800,081 SLAF tags, of which 160,368 were polymorphic. Each tag was 264–364 bp long. The obtained SNPs were used to investigate genetic relationships within Miscanthus. We constructed a phylogenetic tree of the 50 germplasms using the obtained SNPs, and found that the germplasms fell into two clades: one clade of M. sinensis only and one clade that included the offspring, M. lutarioriparius, and M. sacchariflorus. Genetic cluster analysis indicated that M. lutarioriparius germplasm C3 was the most likely male parent of the offspring.Conclusions: As a high-throughput sequencing method, SLAF-seq can be used to identify informative SNPs in Miscanthus germplasms and to rapidly characterize genetic relationships within this genus. Our results will support the development of breeding programs utilizing Miscanthus cultivars with elite biomass- or fiber-production potential.
Zhiyong Chen; Yancen He; Yasir Iqbal; Yanlan Shi; Hongmei Huang; Zili Yi. Investigation of Genetic Relationships within Miscanthus using SNP Markers Identified using SLAF-Seq. 2021, 1 .
AMA StyleZhiyong Chen, Yancen He, Yasir Iqbal, Yanlan Shi, Hongmei Huang, Zili Yi. Investigation of Genetic Relationships within Miscanthus using SNP Markers Identified using SLAF-Seq. . 2021; ():1.
Chicago/Turabian StyleZhiyong Chen; Yancen He; Yasir Iqbal; Yanlan Shi; Hongmei Huang; Zili Yi. 2021. "Investigation of Genetic Relationships within Miscanthus using SNP Markers Identified using SLAF-Seq." , no. : 1.
Yao Li; Yancen He; Yuhuan Lin; Ting Wan; Meng Li; Zhiyong Chen. Bioinformatics Analysis of Cellulose Synthase CesA Gene from Miscanthus lutarioriparius. Molecular Plant Breeding 2021, 12, 1 .
AMA StyleYao Li, Yancen He, Yuhuan Lin, Ting Wan, Meng Li, Zhiyong Chen. Bioinformatics Analysis of Cellulose Synthase CesA Gene from Miscanthus lutarioriparius. Molecular Plant Breeding. 2021; 12 ():1.
Chicago/Turabian StyleYao Li; Yancen He; Yuhuan Lin; Ting Wan; Meng Li; Zhiyong Chen. 2021. "Bioinformatics Analysis of Cellulose Synthase CesA Gene from Miscanthus lutarioriparius." Molecular Plant Breeding 12, no. : 1.