Journal of Medical Internet Research - International Scientific Journal for Medical Research, Information and Communication on the Internet #Preprint #PeerReviewMe: Warning: This is a unreviewed preprint. Readers are warned that the document has not been peer-reviewed by expert/patient reviewers or an academic editor, may contain misleading claims, and is likely to undergo changes before final publication, if accepted, or may have been rejected/withdrawn. Readers with interest and expertise are encouraged to sign up as peer-reviewer, if the paper is within an open peer-review period. Please cite this preprint only for review purposes or for grant applications and CVs (if you are the author). Background: Electronic health (eHealth) information is engrained in the healthcare experience to engage patients across the lifespan. Both eHealth accessibility and optimization are influenced by lifespan development, as older adults experience greater challenges accessing and using eHealth tools as compared to their younger counterparts. The eHealth Literacy Scale (eHEALS) is the most popular measure used to assess patient confidence locating, understanding, evaluating, and acting upon online health information. Currently, however, the factor structure of the eHEALS across discrete age groups is not well understood, which limits its usefulness as a measure of eHealth literacy across the lifespan. Objective: The purpose of this study was to examine the structure of eHEALS scores and the degree of measurement invariance among United States (U.S.) adults representing the following generations: Millennials (18-35 year olds), Generation X (36-51 year olds), Baby Boomers (52-70 year olds), and the Silent Generation (71-84 year olds). Methods: Millennials (N = 281; M = 26.54 years, SD = 5.14 years), Generation X (N = 164; M = 42.97 years, SD = 5.01 years), and Baby Boomers/Silent Generation (N = 384; M = 62.80 years, SD = 6.66 years) members completed the eHEALS. The 3-factor (RMSEA = .06; CFI = .99; TLI = .98) and 4-factor (RSMEA = .06; CFI = .99; TLI = .98) models showed the best global fit, as compared to the 1- and 2-factor models. However, the 4-factor model did not have statistically significant factor loadings on the 4th factor, which led to the acceptance of the 3-factor eHEALS model. The 3-factors included eHealth Information Awareness, Search, and Engagement. Pattern invariance was supported with acceptable model fit (RMSEA = .06; ΔX2 = P > .05; ΔCFI = 0). Compared to Millennials and members of Generation X, those in the Baby Boomer and Silent Generations reported less confidence in their awareness of eHealth resources (P < .001), information seeking skills (P = .003), and ability to evaluate and act on health information found in the Internet (P < .001). Results: Young (18-48 years old; N = 411) and old (49-84 years old; N = 419) adults completed the survey. A 3-factor model had the best fit (RMSEA = .06; CFI = .99; TLI = .98), as compared to the 1-factor, 2-factor, and 4-factor models. These 3-factors included eHealth Information Awareness (2 items), Information Seeking (2 Items), and Information and Evaluation (4 items). Pattern invariance was supported with acceptable model fit (RMSEA = .06; ΔX2 = p > .05; ΔCFI = 0). Compared with younger adults, older adults had less confidence in eHealth resource awareness (p < .001), information seeking skills (p < .01), and ability to evaluate and act upon online health information (p < .001). Conclusions: The eHEALS can be used to uniquely assess, monitor, and evaluate Internet users’ awareness of eHealth resources, information seeking skills, and engagement abilities. Configural and pattern invariance was observed across all generation groups in the 3-factor eHEALS model. To meet gold standards for factor interpretation (i.e., 3 items or indicators per factor), future research is needed to create and assess additional eHEALS items. Future research is also needed to identify and test items for a fourth factor, one that captures the social nature of eHealth.