Identification of essential proteins is always a challenging task since it requires experimental approaches that are time-consuming and laborious. With the advances in high throughput technologies, a large number of protein-protein interactions are available, which have produced unprecedented opportunities for detecting proteins' essentialities from the network level. There have been a series of computational approaches proposed for predicting essential proteins based on network topologies. However, the network topology-based centrality measures are very sensitive to the robustness of network. Therefore, a new robust essential protein discovery method would be of great value.

Regions of interest (ROIs) based classification has been widely investigated for analysis of brain magnetic resonance imaging (MRI) images to assist the diagnosis of Alzheimer's disease (AD) including its early warning and developing stages, e.g., mild cognitive impairment (MCI) including MCI converted to AD (MCIc) and MCI not converted to AD (MCInc). Since an ROI representation of brain structures is obtained either by pre-definition or by adaptive parcellation, the corresponding ROI in different brains can be measured. However, due to noise and small sample size of MRI images, representations generated from single or multiple ROIs may not be sufficient to reveal the underlying anatomical differences between the groups of disease-affected patients and health controls (HC). In this paper, we employ a whole brain hierarchical network (WBHN) to represent each subject. The whole brain of each subject is divided into 90, 54, 14, and 1 regions based on Automated Anatomical Labeling (AAL) atlas. The connectivity between each pair of regions is computed in terms of Pearson's correlation coefficient and used as classification feature. Then, to reduce the dimensionality of features, we select the features with higher F- scores. Finally, we use multiple kernel boosting (MKBoost) algorithm to perform the classification. Our proposed method is evaluated on MRI images of 710 subjects (200 AD, 120 MCIc, 160 MCInc, and 230 HC) from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. The experimental results show that our proposed method achieves an accuracy of 94.65 percent and an area under the receiver operating characteristic (ROC) curve (AUC) of 0.954 forAD/HC classification, an accuracy of 89.63 percent and an AUC of 0.907 forAD/MCI classification, an accuracy of 85.79 percent and an AUC of 0.826 for MCI/HC classification, and an accuracy of 72.08 percent and an AUC of 0.716 for MCIc/MCInc classification, respectively. Our results demonstrate that our proposed method is efficient and promising for clinical applications forthe diagnosis of AD via MRI images.

With the flourish of digital technologies and rapid development of 5G and beyond networks, Metaverse has become an increasingly hotly discussed topic, which offers users with multiple roles for diversified experience interacting with virtual services. How to capture and model users’ multi-platform or cross-space data/behaviors become essential to enrich people with more realistic and immersed experience in Metaverse-enabled smart applications over 5G and beyond networks. In this study, we propose a Personalized Federated Learning with Model-Contrastive Learning (PFL-MCL) framework, which may efficiently enhance the communication and interaction in human-centric Metaverse environments by making use of the large-scale, heterogeneous, and multi-modal Metaverse data. Differing from the conventional Federated Learning (FL) architecture, a multi-center aggregation structure to learn multiple global models based on the changes of dynamically updated local model weights, is developed in global, while a hierarchical neural network structure which includes a personalized module and a federated module to tackle both issues on data heterogeneity and model heterogeneity, is designed in local, so as to enhance the performance of PFL with unique characteristics of Metaverse data. In particular, a two-stage iterative clustering algorithm with a more precise initialization is developed to facilitate the personalized global aggregation with dynamically updated multiple aggregation centers. A personalized multi-modal fusion network is constructed to greatly reduce the computational cost and feature dimensions from the high-dimensional heterogeneous inputs for more efficient cross-modal fusion, based on a hierarchical shift-window attention mechanism and a newly designed bridge attention mechanism. A MCL scheme is then incorporated to speed up the model convergence with less communication overload between the local federated module and global model, while an embedding layer which effectively enables the delivered global model to better adapt to the local personality in each client is further integrated. Compared with five baseline methods, experiment and evaluation results based on two different real-world datasets demonstrate the excellent performance of our proposed PFL-MCL model in a fine-grain personalized training strategy, toward more efficient communication and networking among human-centric Metaverse enabled smart applications.

Background: Identification of essential proteins plays a significant role in understanding minimal requirements for the cellular survival and development. Many computational methods have been proposed for predicting essential proteins by using the topological features of protein-protein interaction (PPI) networks. However, most of these methods ignored intrinsic biological meaning of proteins. Moreover, PPI data contains many false positives and false negatives. To overcome these limitations, recently many research groups have started to focus on identification of essential proteins by integrating PPI networks with other biological information. However, none of their methods has widely been acknowledged. Results: By considering the facts that essential proteins are more evolutionarily conserved than nonessential proteins and essential proteins frequently bind each other, we propose an iteration method for predicting essential proteins by integrating the orthology with PPI networks, named by ION. Differently from other methods, ION identifies essential proteins depending on not only the connections between proteins but also their orthologous properties and features of their neighbors. ION is implemented to predict essential proteins in S. cerevisiae. Experimental results show that ION can achieve higher identification accuracy than eight other existing centrality methods in terms of area under the curve (AUC). Moreover, ION identifies a large amount of essential proteins which have been ignored by eight other existing centrality methods because of their low-connectivity. Many proteins ranked in top 100 by ION are both essential and belong to the complexes with certain biological functions. Furthermore, no matter how many reference organisms were selected, ION outperforms all eight other existing centrality methods. While using as many as possible reference organisms can improve the performance of ION. Additionally, ION also shows good prediction performance in E. coli K-12. Conclusions: The accuracy of predicting essential proteins can be improved by integrating the orthology with PPI networks.

Along with the popularity of the Internet of Things (IoT) techniques with several computational paradigms, such as cloud and edge computing, microservice has been viewed as a promising architecture in large-scale application design and deployment. Due to the limited computing ability of edge devices in distributed IoT, only a small scale of data can be used for model training. In addition, most of the machine-learning-based intrusion detection methods are insufficient when dealing with imbalanced dataset under limited computing resources. In this article, we propose an optimized intra/inter-class-structure-based variational few-shot learning (OICS-VFSL) model to overcome a specific out-of-distribution problem in imbalanced learning, and to improve the microservice-oriented intrusion detection in distributed IoT systems. Following a newly designed VFSL framework, an intra/inter-class optimization scheme is developed using reconstructed feature embeddings, in which the intra-class distance is optimized based on the approximation during a variation Bayesian process, while the inter-class distance is optimized based on the maximization of similarities during a feature concatenation process. An intelligent intrusion detection algorithm is, then, introduced to improve the multiclass classification via a nonlinear neural network. Evaluation experiments are conducted using two public datasets to demonstrate the effectiveness of our proposed model, especially in detecting novel attacks with extremely imbalanced data, compared with four baseline methods.