Thermodynamic characteristics and their effects on single cavitation bubble dynamics are important to elucidate the physical behaviors of cavitation phenomena. In this study, experimental and numerical methods were utilized to explore the thermodynamic effects on single cavitation bubble dynamics under various ambient temperature conditions. A series of experiments was performed to generate a single cavitation bubble at ambient temperatures between 20 and 80 °C using a laser-induced method and a high-speed camera to observe the dynamic behaviors of bubbles. By increasing the ambient temperature, a nonspherical bubble shape with a jet flow at the bubble rebound stage was observed. Next, the numerical simulation results in terms of the bubble radius and bubble shape were validated with the corresponding experimental data. Generally, the results exhibited reasonable agreement, particularly at the later collapse and rebound stages. Critical hydrodynamic and thermodynamic mechanisms over multiple oscillation stages at different ambient temperatures were analyzed. The bubble behaviors and their intensities were numerically quantified with respect to the bubble radius, collapsing time, internal pressure, internal temperature, and phase transition rate parameters. The results showed that the maximum bubble radius, first minimum bubble radius, and collapsing time increased with an increase in the ambient temperature. Nevertheless, the peak values of the internal pressure and internal temperature decreased with an increase in the ambient temperature. Generally, the bubble collapsed less violently at high temperatures than at low temperatures.