Background —Although multiple studies have shown that the left ventricular assist device (LVAD) improves distorted cardiac geometry, the pathological mechanisms of the “reverse remodeling” of the heart are unknown. Our goal was to determine the effects of LVAD support on cardiac myocyte size and shape. Methods and Results —Isolated myocytes were obtained at cardiac transplantation from 30 failing hearts (12 ischemic, 18 nonischemic) without LVAD support, 10 failing hearts that received LVAD support for 75±15 days, and 6 nonfailing hearts. Cardiac myocyte volume, length, width, and thickness were determined by use of previously validated techniques. Isolated myocytes from myopathic hearts exhibited increased volume, length, width, and length-to-thickness ratio compared with normal myocytes ( P <0.05). However, there were no differences in any parameter between myocytes from ischemic and nonischemic cardiomyopathic hearts. Long-term LVAD support resulted in a 28% reduction in myocyte volume, 20% reduction in cell length, 20% reduction in cell width, and 32% reduction in cell length-to-thickness ratio ( P <0.05). In contrast, LVAD support was associated with no change in cell thickness. These cellular changes were associated with reductions in left ventricular dilation and left ventricular mass measured echocardiographically in 6 of 10 LVAD-supported patients. Conclusions —These studies suggest that the regression of cellular hypertrophy is a major contributor to the “reverse remodeling” of the heart after LVAD implantation. The favorable alterations in geometry that occur in parallel fashion at both the organ and cellular levels may contribute to reduced wall stress and improved mechanical performance after LVAD support.

We examined whether the levels of oxidative stress biomarkers measured in blood reflect the tissue redox status. Data from studies that measured redox biomarkers in blood, heart, liver, kidney and skeletal muscle were analyzed. In seven out of nine investigated redox biomarkers (malondialdehyde, reduced glutathione, superoxide dismutase, catalase, glutathione peroxidase, vitamin C and E) there was generally good qualitative and quantitative agreement between the blood and tissues. In contrast, oxidized glutathione and the reduced to oxidized glutathione ratio showed poor agreement between the blood and tissues. This study suggests that most redox biomarkers measured in blood adequately reflect tissue redox status.

This study compared the effects of unilateral and bilateral plyometric training on strength, sprint performance and lower limb power. Sixty-eight preadolescent soccer athletes were randomly assigned to a unilateral plyometric training group (n=23), a bilateral plyometric training group (n=23) and a control group (n=22). Both plyometric training groups trained with equal volumes of unilateral or bilateral exercises for 15 minutes in each session, at which time the control group performed soccer-specific drills. Plyometric exercises were executed twice weekly for 10 weeks during the competitive season. The following tests were performed before and after the intervention: single-leg and double-leg countermovement jump, squat jump, horizontal jumps in different directions, maximal isometric strength of quadriceps and hamstrings, sprint performance, agility and balance. Unilateral plyometric training resulted in greater improvements compared to the control group in the following variables: hamstrings strength (ES: 0.91, p=0.037), 5m sprint time (ES: 0.93, p=0.004), single-leg countermovement jump (ES: 0.90, p=0.006), single- and double-leg squat jump (ES: 0.87, p=0.030 and ES: 0.73, p=0.067, respectively) and single-leg hop performance (ES: 1.01, p=0.004). The only tests where there was an improvement of BPT compared with the CG were the single-leg and double leg SJ (ES: 0.76, p=0.026; ES: 0.70, p=0.050). Quadriceps strength, side hop test, double-leg horizontal jump test, flamingo balance test and modified agility T-test were equally improved in all three groups (p<0.001). In conclusion, unilateral lower-limb plyometric training is more effective in increasing muscle strength and power in preadolescent soccer players when compared to bilateral training or soccer training alone.

Purpose:This study examined the effects of age on recovery of peak torque of knee extensors (PTEX) and flexors (PTFL), and total work (TW) during high-intensity intermittent 30-s (HI30) and 60-s (HI60) exercise in boys (N = 19; age, 11.4 ± 0.5 yr), teens (N = 17; age, 14.7 ± 0.4 yr), and men