Spur dikes provide significant control for flow regimes in river regulation engineering, which can help in the regeneration of stream habitats. However, the narrowing of the flow by spur dike changes the turbulence characteristics. To clarify the turbulence characteristics around the spur dike, the method of large eddy simulation (LES) was used to investigate the horizontal turbulence structure around spur dikes with different discharges in an open-channel flume. The simulations were an exact reproduction of large-scale laboratory experiments, which showed agreement with the experimental results. The distributions of time-averaged streamwise velocity, bed shear stress, and second-order turbulence statistics obtained from the LES were analyzed. An examination of the time series of velocity fluctuation as the probability density function, quadrant analysis, the power density spectra, flow instability, and the vortex separation created in the detached shear layer were estimated. The results accurately revealed the flow field under flow separation, the turbulence statistics inside the separated shear layer, and the vortex structure and emphasized the variation in the different water depths. The results demonstrated that the form of turbulence was not significantly affected by discharge. Moreover, vortex and energy transmission displayed the same periodicity, despite variances in the structural form of turbulence at different water depths. Overall, the results of the study provide an efficient basis for understanding the turbulence around spur dikes, which is crucial for their safe design.