The availability of clean water and the depletion of non-renewable resources provide challenges to modern society. The widespread use of conventional wastewater treatment necessitates significant financial and energy expenditure. Constructed Wetland Microbial Fuel Cells (CW-MFCs), a more recent alternative technology that incorporates a Microbial Fuel Cell (MFC) inside a Constructed Wetland (CW), can alleviate these problems. By utilizing a CW’s inherent redox gradient, MFC can produce electricity while also improving a CW’s capacity for wastewater treatment. Electroactive bacteria in the anaerobic zone oxidize the organic contaminants in the wastewater, releasing electrons and protons in the process. Through an external circuit, these electrons travel to the cathode and produce electricity. Researchers have demonstrated the potential of CW-MFC technology in harnessing bio-electricity from wastewater while achieving pollutant removal at the lab and pilot scales, using both domestic and industrial wastewater. However, several limitations, such as inadequate removal of nitrogen, phosphates, and toxic organic/inorganic pollutants, limits its applicability on a large scale. In addition, the whole system must be well optimized to achieve effective wastewater treatment along with energy, as the ecosystem of the CW-MFC is large, and has diverse biotic and abiotic components which interact with each other in a dynamic manner. Therefore, by modifying important components and optimizing various influencing factors, the performance of this hybrid system in terms of wastewater treatment and power generation can be improved, making CW-MFCs a cost-effective, cleaner, and more sustainable approach for wastewater treatment that can be used in real-world applications in the future.

Reactive Black 5 is one of the most widely used dye in textile and other industries. It is one of the significantly toxic azo dye which poses a serious threat to the environment when discharged into water bodies. A bacterial strain having potential to decolourise and degrade RB5 was isolated from textile effluent, and further identified and characterized. On the basis of morphological, biochemical, and 16s rRNA sequence analysis, the isolate was identified as Bacillus albus DD1. It showed 98% removal of RB5 from aqueous medium within 38 h under optimum parameters, pH 7, temperature 40 °C, in the presence of 1% yeast extract as a co-substrate, and 25% inoculum size at the initial dye concentration of 50 mg/l. Kinetic study revealed the decolorization reaction is a first order non- spontaneous reaction. The rate constant and reaction rate for RB5 decolourization in presence of the isolate was 0.0523 s⁻¹ and 2.6 × 10⁻³ mol/m³ sec, respectively. Values for ΔH and ΔS of the decolourization reaction, determined by thermodynamic analysis, were estimated to be +20.80 kJ/mol and ΔS = −0.1 kJ/mol K, respectively. LC-MS analysis revealed that decolorization was due to degradation of RB5 by cleavage of azo-bond by the bacterium, with the formation of s 3,6,8-trihyroxynapthalene and phthalic acid as degradation products. Therefore, the bacterium Bacillus albus DD1 has potential for application in biological treatment of dye contaminated industrial waste water.