Despite continuous progress in therapy, melanoma is one of the most aggressive and malignant human tumors, often relapsing and metastasizing to almost all organs. Cold atmospheric plasma (CAP) is a novel anticancer tool that utilizes abundant reactive oxygen and nitrogen species (RONS) being deposited on the target cells and tissues. CAP-induced differential effects between non-cancerous and cancer cells were comparatively examined. Melanoma and non-cancerous skin fibroblast cells (counterparts; both cell types were isolated from the same patient) were used for plasma–cell interactions. The production of intracellular RONS, such as nitric oxide (NO), hydroxyl radical (•OH), and hydrogen peroxide (H₂O₂), increased remarkably only in melanoma cancer cells. It was observed that cancer cells morphed from spread to round cell shapes after plasma exposure, suggesting that they were more affected than non-cancerous cells in the same plasma condition. Immediately after both cell types were treated with plasma, there were no differences in the amount of extracellular H₂O₂ production, while Hanks’ balanced salt solution-containing cancer cells had lower concentrations of H₂O₂ than that of non-cancerous cells at 1 h after treatment. The melanoma cells seemed to respond to CAP treatment with a greater rise in RONS and a higher consumption rate of H₂O₂ than homologous non-cancerous cells. These results suggest that differential sensitivities of non-cancerous skin and melanoma cells to CAP-induced RONS can enable the applicability of CAP in anticancer therapy.

Peanut (Arachis hypogaea L.) shell, an abundant by-product of peanut production, contains a complex combination of organic compounds, including flavonoids. Changes in the total phenolic content, flavonoid content, antioxidant capacities, and skin aging-related enzyme (tyrosinase, elastase, and collagenase)-inhibitory activities of peanut shell were investigated after treatment in pressure swing reactors under controlled gas conditions using surface dielectric barrier discharge with different plasma (NO_x and O₃) and temperature (25 and 150 °C) treatments. Plasma treatment under ozone-rich conditions at 150 °C significantly affected the total phenolic (270.70 mg gallic acid equivalent (GAE)/g) and flavonoid (120.02 mg catechin equivalent (CE)/g) contents of peanut shell compared with the control (253.94 and 117.74 mg CE/g, respectively) (p < 0.05). In addition, with the same treatment, an increase in functional compound content clearly enhanced the antioxidant activities of components in peanut shell extracts. However, the NO_x-rich treatment was significantly less effective than the O₃ treatment (p < 0.05) in terms of the total phenolic content, flavonoid content, and antioxidant activities. Similarly, peanut shells treated in the reactor under O₃-rich plasma conditions at 150 ℃ had higher tyrosinase, elastase, and collagenase inhibition rates (55.72%, 85.69%, and 86.43%, respectively) compared to the control (35.81%, 80.78%, and 83.53%, respectively). Our findings revealed that a reactor operated with O₃-rich plasma-activated gas at 150 °C was better-suited for producing functional industrial materials from the by-products of peanuts.

To the best of our knowledge, few studies have utilized cold plasma to improve soybean protein extraction yield and the functional properties of soybean protein. In this study, we aimed to assess the benefits of remote plasma treatments on soybean with respect to the utilization of soybean protein. This study involved two different sample forms (whole and crushed beans), two different plasma chemistry modes (ozone and nitrogen oxides [NO_x = NO + NO₂]), and a novel pressure-swing reactor. Crushed soybeans were significantly affected by NO_x-mode plasma treatment. Crushed soybeans treated with NO_x-mode plasma had the best outcomes, wherein the protein extraction yield increased from 31.64% in the control to 37.90% after plasma treatment. The water binding capacity (205.50%) and oil absorption capacity (267.67%) of plasma-treated soybeans increased to 190.88% and 246.23 % of the control, respectively. The emulsifying activity and emulsion stability slightly increased compared to those of the control. The secondary structure and surface hydrophobicity were altered. The remote plasma treatment of crushed soybeans increased soybean protein extraction yield compared to plasma-treated whole beans as well as untreated beans and altered the structural and physicochemical properties of soybean proteins.

Ozone (O₃), nitrogen oxides (NO _x ), and reactive nitrogen species (RNS) play critical roles in atmospheric-pressure plasma applications. Although it is crucial to individually quantify these species to understand atmospheric-pressure plasmas and increase their effectiveness, the lack of reliable and cost-effective diagnostics makes this difficult for many researchers. To address this problem, we introduce a new deconvolution method of broadband ultraviolet–visible absorption spectra for the simultaneous measurement of eight species—O₃, NO, NO₂, NO₃, N₂O₄, N₂O₅, HONO, and HNO₃. Processing of broadband spectra enables deconvolution of similar cross-section profiles and measurement of high densities exceeding the instrumental limit. Novel correction processes enable accurate analysis despite incomplete cross-section data and utilize a priori chemical knowledge to ensure theoretically reasonable results. Two case studies test the efficacy of the method: NO₂ and N₂O₄ equilibria, and reactive species produced by a surface dielectric barrier discharge. With an analysis time of 15–20 ms per spectrum, the measured densities agree well with other theoretical and experimental results, and detection limits on the order of ppmv were achieved with a short path length of 15 cm. This spectral analysis method will facilitate the real-time monitoring of O₃, NO _x , and RNS in many scientific research and industrial applications of atmospheric pressure plasmas.

Effects of duty ratio of a unipolar microsecond-pulsed helium atmospheric-pressure plasma jet (APPJ) on liquid- and polymer-surface treatments were investigated. In addition, changes in the plasma plume length, gas temperature, excitation temperature, discharge current, absorption power, and optical emission spectra were examined by varying the other operating parameters, such as applied voltage and additive flow of oxygen or water vapor. As an example of liquid sample, de-ionized water (DW) was exposed to an APPJ, and the concentrations of the reactive species generated in the DW were measured as functions of the operating parameters. Polycarbonate, polypropylene, and polymethylmethacrylate were employed as exemplary substrate materials to investigate the effect of plasma treatment on polymeric surfaces. The APPJ treatment increased the surface energy and changed the wetting characteristics of the surface from hydrophobic to hydrophilic. X-ray photoelectron spectroscopy results showed that a short-time plasma treatment with He and/or He/O2, He/H2O affects the surface wettability owing to the introduction of polar groups.

Media exposed to atmospheric pressure plasma (APP) produce reactive oxygen and nitrogen species (RONS), with hydrogen peroxide (H₂O₂), nitrite (NO₂⁻), and nitrate (NO₃⁻) being among the most detected species due to their relatively long lifetime. In this study, a standardized microwave-excited (ME) APP jet (APPJ) source was employed to produce gaseous RONS to treat liquid samples. The source was a commercially available plasma jet, which generated argon plasma utilizing a coaxial transmission line resonator at the operating frequency of 2.45 GHz. An ultraviolet-visible spectrophotometer was used to measure the concentrations of H₂O₂ and NO₃⁻ in plasma-activated media (PAM). Three different types of media (deionized water, Hank’s balanced salt solution, and cell culture solution Dulbecco’s modified eagles medium [DMEM]) were utilized as liquid samples. Among these media, the plasma-treated DMEM was observed to have the highest levels of H₂O₂ and NO₃⁻. Subsequently, the feasibility of using argon ME-APPJ-activated DMEM (PAM) as an adjuvant to enhance the therapeutic effects of cisplatin on human bladder cancer cells (T-24) was investigated. Various cancer cell lines, including T-24 cells, treated with PAM were observed in vitro for changes in cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. A viability reduction was detected in the various cancer cells after incubation in PAM. Furthermore, the study’s results revealed that PAM was effective against cisplatin-resistant T-24 cells in vitro. In addition, a possible connection between HER expression and cell viability was sketched.