Altogether, 20–30% of women receive intrapartum antibiotic prophylaxis (IAP) to prevent sepsis in infants and 2–5% of newborn infants receive antibiotics due to suspected sepsis. Caesarean section has a long-term impact on the intestinal microbiome but the effects of perinatal antibiotics on gut microbiome in vaginally delivered infants are not well known. We compared the impact of IAP, postnatal antibiotics, or their combination on the gut microbiome and emergence of antimicrobial resistance in a controlled study of 149 newborn infants recruited within 24 hours after birth. We collected 659 fecal samples, including 426 daily samples from infants before discharge from the hospital and 111 follow-up samples at six months. Penicillin was mostly used for IAP and the combination of penicillin and aminoglycoside for postnatal treatment. Postnatal antibiotic groups received Lactobacillus reuteri probiotic. Newborn gut colonization differed in both IAP and postnatal antibiotics groups as compared to that in control group. The effect size of IAP was comparable to that caused by postnatal antibiotics. The observed differences were still present at six months and not prevented by lactobacilli consumption. Given the present clinical results, the impact of perinatal antibiotics on the subsequent health of newborn infants should be further evaluated.

Eighty-seven endophytic fungi were isolated from asymptomatic leaf tissues of Rhododendron tomentosum. Most of the isolates were non-sporulating and therefore difficult to identify exclusively based on morphological characters. Eighteen isolates that were morphologically distinct were selected for identification by sequencing the ITS region. For culture-independent analysis, the DNA was isolated from the surface-sterilized leaves of R. tomentosum and the ITS region was amplified using fungal specific primers ITS1F and ITS4, cloned, and 11 clones were randomly selected and sequenced. The phylogenetic analysis was performed with MEGA4 on a total of 49 sequences, including 18 endophytic isolates and 11 unculturables obtained in this study, and 20 sequences from Genbank, which were distributed in four clusters. The culturable and unculturable endophytes formed separate clades and were clearly distinguishable with no overlap within the groups. Endophytic fungi are a well-recognized source of bioactive compounds and therefore antibacterial and antioxidant activities of the isolates of R. tomentosum were studied. All isolates were grown in two different media, enriched (MEB) and depleted (DM), and screened for antibacterial and antioxidant activities. As a result, 10% produced antibacterials and 14% antioxidants, in total 24% of the isolates had biological activity. Antioxidant broth of TRT59 was partially purified using HPLC. The majority of antibacterial compounds were produced in DM media and antioxidants in MEB media. Therefore, it is advisable to test various media for production of antibacterials and antioxidants.

Covering up to the end of August 2013 Phenalenones are members of a unique class of natural polyketides exhibiting diverse biological potential. This is a comprehensive review of 72 phenalenones with diverse structural features originating from fungal sources. Their bioactive potential and structure elucidation are discussed along with a review of their biosynthetic pathways and the taxonomical relationship between the fungi producing these natural products.