The middle and lower Huai River Valley, located between the Yangtze and Yellow Rivers, was a key transitional zone for the northward spread of rice and southward migration of millet agriculture in central-eastern China during the Holocene. Knowing when millets spread here, how they were combined with rice in mixed farming, the reasons for their spread, and the temporal variation of cropping patterns is of crucial significance to the development of our understanding of ancient adaptation strategies adopted by human societies in response to climatic and cultural changes. Focusing on crops, phytolith analyses of the soil samples, in tandem with radiocarbon dating from the Wanbei site, reveal evidence of a multicropping pattern of combining rice (Oryza sative), broomcorn millet (Panicum miliaceum), and foxtail millet (Setaria italica) during the Dawenkou culture period between 5720 and 4426 cal. BP in the middle and lower Huai River Valley, China. The data show that rice was always the principal crop of the pattern, and that domesticated rice was developed during the early and middle Dawenkou culture periods. However, its domestication rate became lower during the late Dawenkou culture period. Broomcorn millet and foxtail millet with domesticated traits appeared only in lower proportions of the total produced throughout the period. The proportions of rice and foxtail millet increased slightly, while the proportions of broomcorn millet decreased over time. Finally, the formation of the multicropping pattern at Wanbei may have been primarily influenced by both the warm and wet climatic environment and the cultural exchange and communication between the Haidai region and the middle and lower Huai River Valley during the Dawenkou culture period. The findings in this paper may not only contribute to mapping the spatiotemporal route for the northward expansion of rice agriculture and southward spread of millet agriculture, but also assist in understanding the human adaptation strategies employed in eastern China during the Holocene.

The site of Zhengzhou Shang City (ca. 1509-1315 cal. BC) was the capital of the early Shang Dynasty in China. Archaeological excavations have unearthed a ditch containing approximately one hundred unusual dead human skulls in the rammed-earth foundations of the palace area. The identity and origin of the skulls have long been disputed. In this work, strontium, carbon and nitrogen stable isotope analyses were carried out on 11 human skulls and 1 tooth from the ditch, as well as on 11 human bones, 11 human teeth from the ordinary tombs and 10 pig teeth from the Zhengzhou Shang City site. We determined that, in Zhengzhou Shang City, the local strontium isotope ratio ranges from 0.711606 to 0.711884, and ordinary inhabitants consumed mainly C₄ plants supplemented by C₃ plants. Moreover, humans buried in the ditch have ⁸⁷Sr/⁸⁶Sr values from 0.711335 to 0.711741 and consumed only C₄ plants. Combining the isotopic data with the archaeological and cultural context, it is concluded that the unusual human skulls in the ditch are most likely those of prisoners of war captured by the central forces conquering the Xiaomintun area of Anyang in the early Shang Dynasty. The results provide valuable insight into the history of violence and military warfare in the early Chinese dynasty.

 Razavi Khorasan Province in the northeast of Iran, located at the Crossroads of Eurasia, was an important point of the middle part of the Silk Road. Neyshabur Plain is situated an important transporting hub of the major thoroughfare of Eurasia. A large number of sites are distributed in the river valleys and the alluvial fans in front of the mountains. Archaeological survey was carried out in Neyshabur Plain, and more than 10 sites were discovered, which are in form of mounds of earth, named as Tape. Seen from the cultural relics on the surface, these sites were occupied by successive cultural sequences, mainly ranging from Neolithic, Chalcolithic, Bronze to Iron Age. This appearance indicates that the climate and environment in the past was better than now. Today, the region is characterized by dry climate, and poor land resources. The land is dominated by Gobi Desert, and the wide vegetation is dominated by Camel thorn (Alhagi sparsifolia). Only in which Karez irrigation system exists, can wheat (Triticum aestivum), barley (Hordeum vulgare) and saffron (Crocus sativus) be cultivated, while a few orchard is present in some river valley areas.    Tape Borj, which is the largest prehistoric settlement site in the east part of Neyshabur plain, Razavi Khorasan Province, NE Iran, covers an area of 13.5 ha. A total area of 110 m2 was excavated in the north and northwest part of the site, and some geological survey were also conducted around the site in 2019. A total of 14 ash pits, 4 houses, 6 ovens, and one well were unearthed during the excavation. According to the AMS dates and material culture, the cultural deposits can be divided into two phases, including Chalcolithic Age during 6500 BP and 6000 BP and early Bronze Age during 5500 BP and 5000 BP. Some wheat, barley, oats (Avena sativa), and seeds of Celtis sinensis, as well as a large number of animal bones, which are dominated by sheep and goats were discovered. The results can basically reflect the economic structure and subsistence strategy of prehistoric ancestors. Geological survey indicates that two paleo river course ever went through the east and west sides of the site during the prehistoric period. In addition, some samples were systematically collected for pollen and phytolith analysis, in order to understand the paleoenvorinment and the utilization of plant resources by the ancient people at the site from the Chalcolithic to the Bronze Age. Our work can provide some precious material data for studying the evolution of the paleoenvrionment and development of agriculture and animal husbandry in this region.

The Jiahu site (ca. 9000–7500 cal. BP) in the Huai River valley has yielded the earliest reported evidence of fermented alcoholic beverages in China. Previous chemical residue analyses interpreted these beverages as a mixture of rice, honey, and fruit, differing markedly from brewing traditions documented at other Neolithic sites in China. To reassess Jiahu brewing practices, we analyzed microfossils preserved in residues from 16 pottery sherds. The residues contained abundant starch granules, phytoliths, and fungal remains, including Monascus cleistothecia, hyphae, and yeast cells, together with starch damage patterns resulting from enzymatic hydrolysis, mashing, and cooking-induced gelatinization. Plant remains included rice, Panicoideae and Triticeae cereals, Job’s tears, foxnut, underground storage organs (USOs), and other taxa. Rice starches and Monascus remains were the most ubiquitous components, indicating that rice and Monascus formed the basis of qu-based brewing, while evidence consistent with malt saccharification was also identified. Together with previous chemical analyses, these findings suggest that Jiahu brewing relied primarily on qu-based saccharification, with contributions from malt-based processing, while honey and fruits may have supplied additional fermentable sugars. Integrating chemical and microfossil evidence provides a robust framework for reconstructing ancient brewing technologies.