The use of biomarkers as early warning systems in the evaluation of disease risk has increased markedly in the last decade. Biomarkers are indicators of typical biological processes, pathogenic processes, or pharmacological reactions to therapy. The application and identification of biomarkers in the medical and clinical fields have an enormous impact on society. In this review, we discuss the history, various definitions, classifications, characteristics, and discovery of biomarkers. Furthermore, the potential application of biomarkers in the diagnosis, prognosis, and treatment of various diseases over the last decade are reviewed. The present review aims to inspire readers to explore new avenues in biomarker research and development.

Current strategies of tissue engineering are focused on the reconstruction and regeneration of damaged or deformed tissues by grafting of cells with scaffolds and biomolecules. Recently, much interest is given to scaffolds which are based on mimic the extracellular matrix that have induced the formation of new tissues. To return functionality of the organ, the presence of a scaffold is essential as a matrix for cell colonization, migration, growth, differentiation and extracellular matrix deposition, until the tissues are totally restored or regenerated. A wide variety of approaches has been developed either in scaffold materials and production procedures or cell sources and cultivation techniques to regenerate the tissues/organs in tissue engineering applications. This study has been conducted to present an overview of the different scaffold fabrication techniques such as solvent casting and particulate leaching, electrospinning, emulsion freeze-drying, thermally induced phase separation, melt molding and rapid prototyping with their properties, limitations, theoretical principles and their prospective in tailoring appropriate micro-nanostructures for tissue regeneration applications. This review also includes discussion on recent works done in the field of tissue engineering.

Cancer is one of the leading causes of death worldwide. Mutation of the cell that regulates genes and protein caused to cancer. Surgery, radiotherapy, and utilize of anticancer agents are the current therapy of cancer despite their side effects. The general area of research relates to heterocyclic chemistry. Purpose of the article is the review of the most recent advances in nitrogen-containing heterocyclics as possible chemotherapy agents for cancer. More than 90% of novel drugs bear heterocyclics that among them nitrogen-containing heterocyclic compounds exhibit superior pharmaceutical effect than non-nitrogen compounds. Nitrogen-containing compounds, the heart of drug discovery, present a significant and valuable category of molecules that play a chief and vital role in the metabolism of living cells.