A rapid and simple HPLC-DAD analytical method was developed and optimized for the determination of anthocynanins in three red Greek winegrape varieties (Kotsifali, Limnio, and Vradiano). The critical parameters, such as the acidifying solvent and the extraction temperature, which affect the extraction of anthocyanins from the grapes, were studied to find the optimum values. The developed methodology was validated in terms of selectivity, linearity, accuracy, and precision and presented satisfactory results. The limits of quantification (LOQs) ranged between 0.20 mg/kg to 0.60 mg/kg, and the limits of detection (LODs) ranged between 0.06 mg/kg and 0.12 mg/kg. The RSD% of the within-day and between-day assays were lower than 6.2% and 8.5%, respectively, showing adequate precision. The accuracy ranged between 91.6 and 119% for within-day assay and between 89.9 and 123% for between-day assay. Sixteen samples from the main regions of each variety as well as from the official ampelographic collections of Greece were collected during the 2020 growing season and were further analyzed by HPLC-DAD. Notable differences in the anthocyanin content were detected among the cultivars using hierarchical cluster analysis (HCA).

Rootstocks serve as a strategic tool for grapevine adaptation to specific biotic and abiotic conditions and for managing vine growth, grape yield, and berry composition in commercial vineyards. This study investigates the influences of four different rootstock varieties (101-14 MGt, 3309 C, 110 R, and 140 Ru) on the viticultural performance of ‘Xinomavro’ vines, a prominent Greek red winegrape varietal. By conducting a two-year field experiment using various rootstocks, we assessed parameters related to water status, vegetative growth, yield, and berry composition. Our results revealed that rootstock selection has a significant impact on vine development, especially in terms of berry size and the concentrations of secondary metabolites. Principal component analysis confirmed the complex interaction between rootstock vigor and vine productivity. This study underscores the importance of rootstock variety in manipulating grapevine characteristics, particularly for the ‘Xinomavro’ variety, in response to regional climatic conditions.

This paper aims to explore the impact of targeted viticultural and enological interventions on reducing the astringency of wines made solely by Mandilaria grape variety. Mandilaria is characterized by its high berry density, high tannin content, in-tense color and full body profile, all of which contribute to the distinctive enological characteristics of the wines while also pretending challenges for producers during vi-nification. This research aims to apply specific practices in the vineyard and the win-ery to reduce the astringency of the variety and adapt the wine produced to the re-quirements of the present consumers demands. In the vineyards of Paros Island, dif-ferent intensities of leaf removal and modifications to pruning load were applied. Three distinct post-harvest grape dehydration techniques and two varying levels of seed removal during alcoholic fermentation were evaluated for their effectiveness in reducing astringency. The results demonstrate that post-harvest dehydration tech-niques, particularly air and sun dehydration, significantly influence the quality indi-cators of Mandilaria wines, enhancing phenolic content, tannin levels, and antioxidant activity, while also improving phenolic ripeness and reducing the harsh tannic profile. Furthermore, seed removal effectively diminished astringency without affecting the wine’s structure. These findings suggest that the integration of these viticultural and enological techniques can significantly enhance the sensory attributes of Mandilaria wines, making them more appealing to modern consumers.

This paper aims to explore the impact of targeted viticultural and enological interventions on reducing the astringency of wines made solely with Mandilaria, a red Vitis Vinifera L. grape variety. Mandilaria is characterized by its high berry density, high tannin content, intense color and full body profile, all of which contribute to the distinctive enological characteristics of the wines while also pretending challenges for producers during vinification. This research aims to improve phenolic ripeness and adapt the wine produced to the requirements of the present consumers demands. In the vineyards of Paros Island, different intensities of leaf removal and modifications to pruning load were applied. Three distinct post-harvest grape dehydration techniques and two varying levels of seed removal during alcoholic fermentation were evaluated for their effectiveness in reducing astringency. Sensory analysis with a trained panel was also performed. The results demonstrate that post-harvest dehydration techniques, particularly air and sun dehydration, significantly influence the quality indicators of Mandilaria wines, enhancing the phenolic content, tannin levels and antioxidant activity, while also improving the phenolic ripeness and reducing the harsh tannic profile. Furthermore, seed removal effectively diminished astringency without affecting the wine’s structure. These findings suggest that the integration of these viticultural and enological techniques can significantly enhance the sensory attributes of Mandilaria wines, making them more appealing to modern consumers.

‘Xinomavro’ (V. vinifera L.) is an important native red wine grape variety in Northern Greece, particularly in PDO (protected designation of origin) regions. Despite its significance, there is limited research on the effects of pruning type and severity on ‘Xinomavro’ vine physiology, yield, and berry quality across diverse environmental conditions. This study aimed to address this knowledge gap and provide growers with crucial information for optimizing vineyard management practices. The study was conducted over two consecutive years (2016 and 2017) in a vineyard in Thessaloniki, Northern Greece. Four treatments (B12: 12 buds on 6 spurs, B24: 24 buds on 12 spurs, M12: 12 buds on 2 canes, and M24: 24 buds on 4 canes) combining two bud load levels (12 or 24 count nodes) and two pruning types (short spurs or long canes) were applied to ‘Xinomavro’ vines in a complete block randomized design. The vine water status, gas exchange, canopy characteristics, yield components, and berry composition were measured. Bud load and pruning type significantly influenced vine canopy development, microclimate, and yield components. Short pruning with high bud load (B24) resulted in denser canopies and higher yields, whereas cane pruning (M12 and M24) led to more open canopies and improved berry quality indicators. Treatment effects on berry composition were inconsistent across years but showed a tendency for higher anthocyanin and total phenol content in cane-pruned vines. This study demonstrates that pruning type (short or long fruiting units) may have a greater impact on vine growth, yield, and berry composition than bud load alone in ‘Xinomavro’ vines. Cane pruning appears to be a more effective strategy for achieving vine balance and potentially improving grape quality under given experimental conditions.

This study focuses on identifying wine-growing subzones within the PDO Amyndeon and PGI Drama wine-growing zones in Northern Greece, with the aim of assessing their suitability for producing high-quality red wines from the Xinomavro (Vitis vinifera L.) and Cabernet Sauvignon (Vitis vinifera L.) grape varieties, respectively. The initial delineation of suitability zones was carried out using readily available satellite data on soil, topography, and climate, in four different suitability categories. To validate how effectively these categories distinguished actual wine-growing regions, we compared them against two years of field data collected from experimental vineyards. The results showed that this methodology was able to discern the most suitable areas for both varieties and regions with an acceptable relation to real grape and wine attributes as confirmed by the collection of data from the pilot vineyards. The overall performance of this method will ultimately depend on the validity of the expert knowledge used to define the most critical parameters and their range. According to the results of this study, and given the relevance of the proposed suitability criteria, this method has the potential to provide an alternative solution for subzone delineation in cases where wine analytical and sensory data are not available.