Soil microbes play an important role in plant invasion, and parasitic plants regulate the growth of invasive plants. However, the mechanisms by which parasitic plants regulate the effects of soil microbes on invasive plants have not been investigated. Here, we used the invasive plant Alternanthera philoxeroides and the holoparasitic plant Cuscuta grovonii to test whether and how C. grovonii parasitism shifts the effect of native soil microbes on the growth of A. philoxeroides. In a factorial setup, A. philoxeroides was grown in pots with the presence versus absence of parasitism and the presence versus absence of native soil microbes. The findings showed that native soil microbes increased the biomass and clonal growth of A. philoxeroides only in the absence of a parasite, whereas parasitism decreased the biomass and clonal growth of A. philoxeroides only in the presence of soil microbes. In addition, the presence of soil microbes increased the deleterious effects of the parasite on A. philoxeroides. These results indicate that parasitism can shift the effects of native soil microbes on the growth of the invasive plant A. philoxeroides. Our results enrich the understanding of the mechanisms underlying the success of plant invasion.

Plants infected by parasitic species might be further stressed by climate warming as both factors can influence the host plant's nutrient acquisition and growth. The root economics space, defined by root functional traits, reflects a plant's nutrient acquisition strategy. However, the combined effects of warming and parasitism on root functional traits and their positions within the root economics space have not been extensively studies. We grew soybean plants in pot cultures outdoor in the absence or presence of parasitism by Cuscuta gronovii and with or without simulated climate warming using infrared heater lamps. We measured various root functional traits of soybean, including root morphological traits, symbiosis with arbuscular mycorrhizal fungi (AMF) and rhizobia and enzyme activity. Correlations among these traits were analysed, and principal component analysis was used to determine the position of the plants in the root economics space. Parasitism significantly reduced AMF colonization rate, nodule biomass, root tissue density (RTD) and phosphatase activity in the rhizosheath, while increasing root nitrogen concentration (RN). Nodule biomass was positively correlated with AMF colonization, indicating an ‘outsourcing’ strategy for nutrient foraging. Parasitized soybeans occupied a trait space with higher RN and lower RTD, indicative of a ‘fast’ nutrient acquisition strategy. Warming did not significantly affect root functional traits or the plants' positions in the root economics space, regardless of parasitism presence. This study is the first to demonstrate that rhizobial colonization, similar to AMF colonization, is part of the ‘outsourcing’ strategy and that parasitism shifts host plants from a ‘slow’ to a ‘fast’ nutrient uptake strategy. These findings provide new insights into plant below‐ground strategies under warming and parasitism. Read the free Plain Language Summary for this article on the Journal blog.