The aim of this project was to examine the effect of microneedle rollers on the percutaneous penetration of tiagabine hydrochloride and carbamazepine across porcine skin in vitro. Liquid chromatography-mass spectrometric analysis was carried out using an Agilent 1200 Series HPLC system coupled to an Agilent G1969A TOF-MS system. Transdermal flux values of the drugs were determined from the steady-state portion of the cumulative amount versus time curves. Following twelve hours of microneedle roller application, there was a 6.74-fold increase in the percutaneous penetration of tiagabine hydrochloride (86.42 ± 25.66 µg/cm²/h) compared to passive delivery (12.83 ± 6.30 µg/cm²/h). For carbamazepine in 20% ethanol, passive transdermal flux of 7.85 ± 0.60 µg/cm²/h was observed compared to 10.85 ± 0.11 µg/cm²/h after microneedle treatment. Carbamazepine reconstituted in 30% ethanol resulted in only a 1.19-fold increase in drug permeation across porcine skin (36.73 ± 1.83 µg/cm²/h versus 30.74 ± 1.32 µg/cm²/h). Differences in flux values of untreated and microneedle-treated porcine skin using solid microneedles for the transdermal delivery of tiagabine were statistically significant. Although there were 1.38- and 1.19-fold increases in transdermal flux values of carbamazepine when applied as 20% and 30% ethanol solutions across microneedle-treated porcine skin, respectively, the increases were not statistically significant.

Plant‐parasitic nematodes are among the most destructive plant pathogens, resulting in a global annual economic loss of about 358 billion dollars. Using synthetic nematicides to control plant‐parasitic nematodes has resulted in broad‐spectrum toxicity to the environment. Plant‐derived secondary metabolites have recently emerged as viable options that provide effective, greener, and renewable routes for managing plant‐parasitic nematodes in various cropping systems. However, limited comprehensive information on plant‐derived secondary metabolites sources, chemical structures, and nematicidal activities is available. This study aims to compile and analyze data on plant‐based secondary metabolites with nematicidal properties collected over the last two decades. In this review, we identified 262 plant‐based metabolites with nematicidal activities that were isolated from 35 plant families and 65 plant species. Alkaloids, terpenoids, saponins, flavonoids, coumarins, thiophenes, and annonaceous acetogenins were among the most studied compounds. In addition to the structure–activity relation for specific metabolites with nematicidal potency, various techniques for their extraction and isolation from plant material are discussed. Our findings demonstrate the potential of plants as a feedstock for sourcing nematicidal compounds and discovering new chemistries that could potentially be used for developing the next generation of nematicides. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Solanum glycoalkaloids are gaining increased scientific attention due to their bioactive potential in the defense of plants against pests and pathogens. The comprehensive glycoalkaloid profiling from the leaves, stems, and roots of seven underexploited Solanum species (S. caripense, S. melanocerasum, S. muricatum, S. nigrum, S. quitoense, S. retroflexum, and S. sisymbriifolium) was conducted using high-performance liquid chromatography–time-of-flight mass spectrometry. A total of 51 glycoalkaloids were shared among the studied Solanum species, with concentrations ranging from 7 to 5.63 × 10⁵ ng g⁻¹. Based on the glycoalkaloid composition, plants were separated into two clusters, Cluster 1 (S. melanocerasum, S. nigrum, and S. retroflexum) and Cluster 2 (S. caripense, S. muricatum, S. quitoense, and S. sisymbriifolium). The inhibition activity of glycoalkaloid extracts on acetylcholinesterase showed a half-maximal inhibitory concentration (IC₅₀), ranging from 0.4 (S. nigrum stems) to 344.9 µg mL⁻¹ (S. sisymbriifolium leaves), that was not directly correlated to the total glycoalkaloid contents. This suggests that the composition of glycoalkaloids in the plant extract, rather than the total concentration, is a driver of biological activity. The study provides a framework for the bioprospecting of underexploited Solanum species for exploring bioactive glycoalkaloids and other compounds with potential pesticidal activities for the development of green bioformulation. This is the first comprehensive report on the glycoalkaloid profiles of S. retroflexum.