The seed germination and seedling growth of rapeseed are crucial stages in plant life, especially when facing abiotic stresses. In the present work, the effects of water and temperature on seed germination and seedling growth were investigated in a rapeseed crop (Brassica napus L.). The plants were examined under different temperature levels (5 °C, 10 °C, 15 °C, 20 °C, 25 °C, 30 °C, and 35 °C) and water levels (twenty-nine levels based on either one-milliliter intervals or as a percentage of the thousand-kernel weight (TKW)). Moreover, planting densities and antifungal application techniques were investigated in the study. The findings demonstrated substantial variations between all the growth parameters investigated at all the tested temperatures, and 20 °C was considered the optimum within a broad range of 15–25 °C. Water availability plays a significant role in germination, which can be initiated at 0.65 mL, corresponding to 500% of the TKW. The method of TKW is a more accurate aspect of water application because of the consideration of the seed weight and size. The optimal water range for the accumulation of dry weight, 3.85–5.9 mL (2900–4400% of TKW), was greater than that required for seedling growth, 1.45–3.05 mL (1100–2300% of TKW). Twenty to twenty-five seeds per 9 cm Petri dish exhibited the most outstanding values compared to the others, which provides an advantage in breeding programs, especially when there are seed limitations. Seed priming is a more effective antifungal application strategy. These data can be incorporated into future rapeseed germination in vitro studies, breeding programs, and sowing date predictions.

Abiotic variables are crucial for seed germination and seedling development. In the present work, we attempted to determine the optimal conditions (temperature, water, seed density, and fungal growth) for sunflower seed development (Helianthus annus L. Larissza). The germination of sunflower seeds was investigated under controlled conditions at eight consistent temperatures: 5 °C, 10 °C, 15 °C, 20 °C, 25 °C, 30 °C, 35 °C, and 40 °C. For the water test, there were 12 water levels based on one-milliliter intervals and 18 water levels based on thousand kernel weight (TKW). In addition, four seed densities (6, 8, 10, and 12) and two antifungal application techniques (sterilization and growing medium) were examined. The results showed that temperature has a significant effect on seed germination, germination timing, and seedling development. Temperatures between 15 and 35 degrees Celsius were optimal for germination, with 25 degrees Celsius being the optimal temperature for significant germination and seedling development. Beginning at 0.6 mL, or 125% of the TKW, sunflower seeds can germinate under a wide range of water availability. The optimal range for seedling development (8.2–11.4) is wider than the optimal range for dry matter accumulation, which is 5.8–8.2 mL or 1000–1625% of the TKW. The finding that a density of 10 to 12 seeds per 9 cm Petri dish demonstrates the most exceptional values is advantageous for future research and breeding projects, particularly when seeds are scarce. Seed priming is a more effective antifungal application technique than other techniques.

This study aimed to determine the optimal water, temperature, and density conditions, alongside antifungal treatments, for pea (Pisum sativum L.) germination in a laboratory setting, with implications for research, breeding, and microgreen production. Germination and early seedling growth were assessed across various temperatures (5 °C to 40 °C), water levels (0–14 mL per Petri dish), seed densities (5, 7, 9, and 11 seeds per Petri dish), and antifungal treatments (Hypo and Bordeaux mixture). The results indicated that optimal germination occurred between 15 °C and 25 °C, with peak performance at 25 °C. Water levels between 7 and 11 mL per 9 cm diameter Petri dish supported robust root and shoot development, while minimal water levels initiated germination but did not sustain growth. Five seeds per Petri dish was optimal for healthy development, whereas higher densities led to increased competition and variable outcomes. Antifungal treatments showed slight improvements in germination and growth, though differences were not statistically significant compared to controls. The study’s novelty lies in its holistic approach to evaluating multiple factors affecting pea germination, offering practical guidelines for enhancing germination rates and seedling vigor. These findings support efficient and resilient crop production systems adaptable to varying environmental conditions, contributing to sustainable agriculture and food security. Future research should explore these factors in field settings and across different pea cultivars to validate and refine the recommendations.

Temperature stress significantly affects seed germination and seedling development by disrupting physiological and biochemical processes in peanuts and chickpeas. Understanding these effects is crucial for improving germination efficiency, optimizing seedling establishment, and developing stress-tolerant crops, which are essential for ensuring agricultural productivity under changing climatic conditions. Therefore, this study, conducted in 2023, examines the germination and seedling development of peanut and chickpea seeds under abiotic stress. Temperature ranges of 5 - 10 - 15 - 20 - 25 - 30 - 35 - 40 - and 45 0C were used. Temperature significantly influenced the germination percentage, germination duration, and seedling development of both crops. The ideal level for peanuts is 25 °C, with a range from 20 to 30 °C. The ideal temperature for the laboratory experiment of chickpeas is 20 Celsius degrees, with a more comprehensive range from 15 to 20 °C. Lower temperatures affect the seedling development rate and germination, while higher temperatures negatively impact germination percentage. This study is unique due to its focus on the crop, its comparison to other research, and the specific levels and types of abiotic stress applied.