Nanomaterials have demonstrated a wide range of applications and recently, novel biomedical studies are devoted to improving the functionality and effectivity of traditional and unmodified systems, either drug carriers and common scaffolds for tissue engineering or advanced hydrogels for wound healing purposes. In this regard, metal oxide nanoparticles show great potential as versatile tools in biomedical science. In particular, iron oxide nanoparticles with different shape and sizes hold outstanding physiochemical characteristics, such as high specific area and porous structure that make them idoneous nanomaterials to be used in diverse aspects of medicine and biological systems. Moreover, due to the high thermal stability and mechanical strength of Fe₂O₃, they have been combined with several polymers and employed for various nano-treatments for specific human diseases. This review is focused on summarizing the applications of Fe₂O₃-based nanocomposites in the biomedical field, including nanocarriers for drug delivery, tissue engineering, and wound healing. Additionally, their structure, magnetic properties, biocompatibility, and toxicity will be discussed.

An electrochemical aptasensor has been developed to determine breast cancer biomarkers (CA 15-3). Aptamer chains were immobilized on the surface of the electrode by g-C₃N₄/Fe₃O₄ nanoparticles, which increased the conductivity and active surface area of the electrode. X-ray diffraction analysis (XRD), Fourier-transformed infrared spectroscopy (FTIR), and transmission electron microscopy (TEM) measurements have been carried out to characterize the nanomaterials. Cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy have been used to characterize the developed electrode. The results demonstrate that the modified electrode has better selectivity for CA 15-3 compared to other biological molecules. It has a good electrochemical response to CA 15-3 with a detection limit of 0.2 UmL⁻¹ and a linear response between 1 and 9 UmL⁻¹. It has been used as a label-free sensor in potassium ferrocyanide medium and as methylene blue-labeled in phosphate buffer medium. This electrode was successfully applied to analyze the serum of diseased and healthy individuals, which corroborates its high potential for biosensing applications, especially for the diagnosis of breast cancer.

Nowadays, diagnosing early-stage cancers can be vital for saving patients and dramatically decreases mortality rates. Therefore, specificity and sensitivity in the detection of cancer antigens should be elaborately ensured. Some early-stage cancers can be diagnosed via detecting the cancer antigen CA-125, such as ovarian cancer, and required treatments can be applied more efficiently. Thus, detection of CA-125 by employing various optical or electrochemical biosensors is a preliminary and crucial step to treating cancers. In this review, a diverse range of optical and electrochemical means of detecting CA-125 are reviewed. Furthermore, an applicable comparison of their performance and sensitivity is provided, several commercial detection kits are investigated, and their applications are compared and discussed to determine whether they are applicable and accurate enough.