Freshwater diaptomid copepods in Cambodia are poorly studied, and only seven taxa were known previously. From February to October 2007, 255 samples were collected from 237 freshwater sites in nine provinces representing four regions (northwest, Cardamom and Elephant Mountains, Mekong Lowlands, and east) of Cambodia. Diversity, seasonal occurrence, geographical distribution of each species, and a checklist of diaptomid copepods are documented. In this case, 24 species were recorded, including two undescribed species belonging to the genera Phyllodiaptomus and Tropodiaptomus. In this case, 14 species are recorded for the first time in Cambodia, and Mongolodiaptomus formosanus has been recorded for the first time in Southeast Asia. One species appears to be endemic to Cambodia and nine to the lower Mekong River Basin. Mongolodiaptomus malaindosinensis was the most common, frequently recorded throughout the four regions (40.5% of the sampled sites), followed by Vietodiaptomus blachei (39.2%), Eodiaptomus phuvongi (38.8%), Mongolodiaptomus formosanus (31.2%), and Eodiaptomus draconisignivomi (27.4%). Very rare species such as Eodiaptomus sanoamuangae, Tropodiaptomus vicinus, Mongolodiaptomus calcarus, and Tropodiaptomus sp. were only recorded at 0.4% to 0.8% of the sampled sites. The species diversity of Cambodia is most similar to that of Thailand, where 22 species (91.6%) occur both in Cambodia and Thailand, and 12 (50%) of the species have been recorded in both Cambodia and Vietnam.

Currently, the synonymy of the two morphologically analogous copepod species, Mongolodiaptomus birulai (Rylov, 1922) and M. formosanus Kiefer, 1937, remains doubtful. While examining the detailed morphology of diaptomid copepods from China, Cambodia, and Vietnam, we came across two undescribed species whose morphology is closely similar to that of M. birulai from Northeast China and M. formosanus from Taiwan. In this paper, descriptions of M. parabirulai sp. nov. from Yunnan, Southwestern China, and M. longiserratus sp. nov. from Cambodia and Vietnam are presented. The first new species, M. parabirulai sp. nov., can be distinguished from its congeners in the male P5 by it having the distal outer portion of the second right exopod produce a wing-shaped expansion and its strongly robust left and right basis. The second new species, M. longiserratus sp. nov., can be differentiated by the following characteristics in the male: the spiniform process on segment 20 of the right antennule is longer than that of segment 21 and has a serrate outer margin, and the right P5 basis has two (longitudinal and semicircular) hyaline lamellae. Furthermore, the status of M. birulai and M. formosanus is reviewed and recommended.