Salt stress is one of the most prominent abiotic stresses. Behind the intricate adaptive responses of plants to salt stress, the regulation of gene expression assumes a pivotal role. Complementing transcriptional mechanisms, post-transcriptional regulation performed by RNA-binding proteins provides an additional layer of control through sophisticated molecular machinery. RBPs interact with both RNA molecules and protein partners to coordinate RNA metabolism and, thus, fine-tune the expression of salt-responsive genes, enabling plants to rapidly adapt to ionic challenges. This review systematically evaluates the functional roles of RBPs localized in distinct subcellular compartments, including nuclear, cytoplasmic, chloroplastic, and mitochondrial systems, in mediating post-transcriptional regulatory networks under salinity challenges. Specific classes of RBPs are discussed in detail, including glycine-rich RNA-binding proteins (GR-RBPs), serine/arginine-rich splicing factors (SR proteins), zinc finger domain-containing proteins, DEAD-box RNA helicases (DBRHs), KH domain-containing proteins, Pumilio domain-containing proteins (PUMs), pentatricopeptide repeat proteins (PPRs), and RBPs involved in cytoplasmic RNA granule formation. By integrating their subcellular localization and current mechanistic insights, this review concludes by summarizing the current knowledge and highlighting potential future research directions, aiming to inspire further investigations into the complex network of RBPs in modulating plant responses to salt stress and facilitating the development of strategies to enhance plant salt tolerance.

Solanum torvum, a wild relative of eggplant in the Solanaceae family, is widely used as a rootstock to prevent various soil-borne diseases. Despite this valued trait, S. torvum seeds exhibit strong seed dormancy, resulting in low-frequency germination and uneven emergence rates during agricultural production. In this study, exogenous gibberellin (GA) was applied to induce the germination of seeds, thereby enabling the application of transcriptome analysis to explore the effects of exogenous GA on germination. A total of 9723 genes were identified to be involved in GA-induced germination of S. torvum seeds. These GA-associated genes were enriched in amino sugar and nucleotide sugar metabolism, glycolysis, the citrate cycle, pyruvate metabolism, hormone biosynthesis, and signaling transduction. Further analysis of these genes reveals that exogenous GA impaired the endogenous biosynthesis of GA. Analysis of the 285 key genes influenced by GA, auxin, abscisic acid (ABA), and other hormones suggests mutual crosstalk in the signaling of various hormones, enabling the joint regulation of the germination of S. torvum seeds. Additionally, a total of 440 genes encoding transcription factors were identified to be responsible for transcriptional reprogramming during GA-induced seed germination, and putative candidate genes responsible for S. torvum seed dormancy and germination, including ABI5, DOG1, DRM1, LEC1, and PIF, were significantly down-regulated in germinated S. torvum seeds. These findings provide the first insights into transcriptome regulation during GA-induced germination of S. torvum seeds.

Ralstonia solanacearum is a highly destructive soil-borne bacterium that causes bacterial wilt disease in more than 310 plant species worldwide. The pathogenicity of the bacteria is closely associated with type III effectors (T3Es), a class of virulence factors that are delivered to host plant cells by the type III secretion system. In spite of the complex evolutionary history and genetic diversity of the R. solanacearum species complex (RSSC), more than 100 different T3Es have been identified from the genomes of various strains. Based on the available functional studies, certain T3Es interact with host plant proteins and suppress host cell immunity, whereas other T3Es are recognized by the host plant to trigger specific resistance mechanisms. This review summarizes the mechanisms by which T3Es interfere with plant immune responses and the activation of the plant defense system upon T3E recognition. This in-depth review of the molecular interactions between R. solanacearum and its host plants offers insights into the complexity of plant–pathogen interactions and provides a scientific rationale and theoretical support for the future breeding of resistant crops.

Subcellular mRNA localization is an evolutionarily conserved mechanism to spatially and temporally drive local translation and, in turn, protein targeting. Hence, this mechanism achieves precise control of gene expression and establishes functional and structural networks during cell growth and development as well as during stimuli response. Since its discovery in ascidian eggs, mRNA localization has been extensively studied in animal and yeast cells. Although our knowledge of subcellular mRNA localization in plant cells lags considerably behind other biological systems, mRNA localization to the endoplasmic reticulum (ER) has also been well established since its discovery in cereal endosperm cells in the early 1990s. Storage protein mRNA targeting to distinct subdomains of the ER determines efficient accumulation of the corresponding proteins in different endosomal storage sites and, in turn, underlies storage organelle biogenesis in cereal grains. The targeting process requires the presence of RNA localization elements, also called zipcodes, and specific RNA-binding proteins that recognize and bind these zipcodes and recruit other factors to mediate active transport. Here, we review the current knowledge of the mechanisms and functions of mRNA localization to the ER in plant cells and address directions for future research.

Soil salinization causes osmotic stress and ionic toxicity in plant cells and severely restricts crop growth and global agricultural productivity. The actin cytoskeleton functions as a rapidly responsive structural module and core signal integrator, with fast structural remodeling triggered upon salt exposure. This review systematically characterizes salt-induced actin rearrangement profiles and dissects the regulatory functions of key actin-binding proteins (ABPs) in governing actin nucleation, severing, bundling and steady-state turnover under saline conditions. Cross-talk among interconnected signaling cascades, including Ca²⁺ signals, the salt overly sensitive (SOS) pathway, reactive oxygen species (ROS) and abscisic acid (ABA) signaling, that orchestrate actin dynamics is synthesized. Key findings highlight that actin-binding proteins act as central molecular hubs linking upstream stress signaling to downstream adaptive outputs, and that actin remodeling underpins multiple salt-tolerance-related physiological events, such as cellular ion homeostasis, plastic root growth, vesicle trafficking, and the modulation of salt-triggered premature programmed cell death. Beyond actin-specific pathways, reciprocal functional interplays between actin filaments and microtubules jointly fine-tune plant salt acclimation. Unresolved questions are discussed and future research directions are proposed to bridge the gap between cytoskeletal dynamics and practical strategies for developing salt-tolerant crops. Overall, the novelty of this review lies in its salt stress-centered, multi-faceted integration of actin dynamics, from actin itself and its remodeling regulators to signaling crosstalk, physiological outputs, and actin–microtubule interplay. This review constructs a comprehensive theoretical framework linking cytoskeletal dynamics to plant salt adaptation and outlines research directions that may inform future strategies for developing salt-tolerant crops.