Hydrogen (H₂), as a source of energy, continues to be a compelling choice in applications ranging from fuel cells and propulsion systems to feedstock for chemical, metallurgical and other industrial processes. H₂, being a clean, reliable, and affordable source, is finding ever increasing use in distributed electric power generation and H₂ fuelled cars. Although still under 0.1%, the distributed use of H₂ is the fastest growing area. In distributed H₂ storage, distribution, and consumption, safety continues to be a critical aspect. Affordable safety systems for distributed H₂ applications are critical for the H₂ economy to take hold. Advances in H₂ sensors are driven by specificity, reliability, repeatability, stability, cost, size, response time, recovery time, operating temperature, humidity range, and power consumption. Ambient temperature sensors for H₂ detection are increasingly being explored as they offer specificity, stability and robustness of high temperature sensors with lower operational costs and significantly longer operational lifetimes. This review summarizes and highlights recent developments in room temperature H₂ sensors.

Gene editing tools have triggered a revolutionary transformation in the realms of cellular and molecular physiology, serving as a fundamental cornerstone for the evolution of disease models and assays in cell culture reactions, marked by various enhancements. Concurrently, microfluidics has emerged over recent decades as a versatile technology capable of elevating performance and reducing costs in daily experiments across diverse scientific disciplines, with a pronounced impact on cell biology. The amalgamation of these groundbreaking techniques holds the potential to amplify the generation of stable cell lines and the production of extracellular matrix hydrogels. These hydrogels, assuming a pivotal role in isolating cells at the single-cell level, facilitate a myriad of analyses. This study presents a novel method that seamlessly integrates CRISPR-Cas9 gene editing techniques with single-cell isolation methods in induced pluripotent stem cell (hiPSC) lines, utilizing the combined power of droplets and hydrogels. This innovative approach is designed to optimize clonal selection, thereby concurrently reducing costs and the time required for generating a stable genetically modified cell line. By bridging the advancements in gene editing and microfluidic technologies, our approach not only holds significant promise for the development of disease models and assays but also addresses the crucial need for efficient single-cell isolation. This integration contributes to streamlining processes, making it a transformative method with implications for enhancing the efficiency and cost-effectiveness of stable cell line generation. As we navigate the intersection of gene editing and microfluidics, our study marks a significant stride toward innovative methodologies in the dynamic landscape of cellular and molecular physiology research.