Cadmium (Cd) is a widespread toxic heavy metal pollutant in agricultural soil, and Cd accumulation in rice grains is a major intake source of Cd for Asian populations that adversely affect human health. However, the molecular mechanism underlying Cd uptake, translocation and accumulation has not been fully understood in rice plants. In this study, a mutant displaying extremely low Cd accumulation (lcd1) in rice plant and grain was generated by EMS mutagenesis from indica rice cultivar 9311 seeds. The candidate SNPs associated with low Cd accumulation phenotype in the lcd1 mutant were identified by MutMap and the transcriptome changes between lcd1 and WT under Cd exposure were analyzed by RNA-seq. The lcd1 mutant had lower Cd uptake and accumulation in rice root and shoot, as well as less growth inhibition compared with WT in the presence of 5 μM Cd. Genetic analysis showed that lcd1 was a single locus recessive mutation. The SNP responsible for low Cd accumulation in the lcd1 mutant located at position 8,887,787 on chromosome 7, corresponding to the seventh exon of OsNRAMP5. This SNP led to a Pro236Leu amino acid substitution in the highly conserved region of OsNRAMP5 in the lcd1 mutant. A total of 1208 genes were differentially expressed between lcd1 and WT roots under Cd exposure, and DEGs were enriched in transmembrane transport process GO term. Increased OsHMA3 expression probably adds to the effect of OsNRAMP5 mutation to account for the significant decreases in Cd accumulation in rice plant and grain of the lcd1 mutant. An extremely low Cd mutant lcd1 was isolated and identified using MutMap and RNA-seq. A Pro236Leu amino acid substitution in the highly conserved region of OsNRAMP5 is likely responsible for low Cd accumulation in the lcd1 mutant. This work provides more insight into the mechanism of Cd uptake and accumulation in rice, and will be helpful for developing low Cd accumulation rice by marker-assisted breeding.

Rice yield estimation is vital for enhancing food security, optimizing agricultural management, and promoting sustainable development. However, traditional satellite/aerial and ground-based/tower-based platforms face limitations in rice yield estimation, and few studies have explored the potential of UAV-borne hyperspectral remote sensing for this purpose. In this study, we employed a UAV-borne push-broom hyperspectral camera to acquire remote sensing data of rice fields during the filling stage, and the machine learning regression algorithms were applied to rice yield estimation. The research comprised three parts: hyperspectral data preprocessing, spectral feature extraction, and model construction. To begin, the preprocessing of hyperspectral data involved geometric distortion correction, relative radiometric calibration, and rice canopy mask construction. Challenges in geometric distortion correction were addressed by tracking linear features during flight and applying a single-line correction method. Additionally, the NIR reflectance threshold method was applied for rice canopy mask construction, which was subsequently utilized for average reflectance extraction. Then, spectral feature extraction was carried out to reduce multicollinearity in the hyperspectral data. Recursive feature elimination (RFE) was then employed to identify the optimal feature set for model performance. Finally, six machine learning regression models (SVR, RFR, AdaBoost, XGBoost, Ridge, and PLSR) were used for rice yield estimation, achieving significant results. PLSR showed the best R² of 0.827 with selected features, while XGBoost had the best R² of 0.827 with full features. In addition, the spatial distribution of absolute error in rice yield estimation was assessed. The results suggested that this UAV-borne imaging hyperspectral-based approach held great potential for crop yield estimation, not only for rice but also for other crops.

Polypogon fugax is a common winter weed in China and other Asia countries. We have previously found a P. fugax biotype (R) resistant to acetyl co-enzyme A carboxylase (ACCase) herbicides also cannot be effectively controlled by some acetolactate synthase (ALS) herbicides. This study evaluated the level of resistance to four ALS herbicides (metsulfuron-methyl, chlorsulfuron, monosulfuron, pyribambenz isopropyl) in the R biotype and the associated resistance mechanism. The R biotype exhibited moderate level of resistance to metsulfuron-methyl (6.0-fold) compared with the sensitive biotype (S). Sequence analysis of ALS gene revealed that two ALS genes existed in P. fugax. However, no substitution associated with ALS resistance mechanism were found in ALS genes between the S and R biotypes. The activity of ALS enzyme isolated from the R biotype was inherently higher and less sensitive to metsulfuron-methyl than the S biotype. Glutathione S-transferases (GST) activity was also less sensitive to metsulfuron-methyl in the R than as the S biotypes. Malathion, a cytochrome P450 (CYP) monooxygenase inhibitor, had much greater synergistic effect with metsulfuron-methyl on the R than as the S plants, reducing the ED₅₀ value (herbicide dose to inhibit growth by 50%) of metsulfuron-methyl by 23- and 6-fold, respectively, suggesting that CYP mediated enhanced metabolism might contribute to the resistance to ALS herbicides. These results suggest that metsulfuron-methyl resistance in the R biotype was associated with the up-regulated ALS enzymatic activity and the GST and CYP-mediated enhanced herbicide metabolism.

Bipolaris eleusines was mixed with herbicides to improve the control of barnyardgrass (Echinochloa crus-galli), a noxious weed in rice fields. The compatibility of B. eleusines with herbicides was evaluated for toxic effects on spore germination and mycelium growth in vitro tests, and varied effects were observed with different chemical products. Briefly, 25 g/L penoxsulam OD plus 10% bensulfuron-methyl WP were much more compatible with B. eleusines, and there was no inhibition of spore germination but the promotion of mycelium growth of B. eleusines at all treatment rates. Under greenhouse conditions, the coefficient of the specificity of B. eleusines conidial agent was determined as 3.91, closer to the herbicidal control of 2.89, showing it is highly specific between rice and barnyardgrass. Field experiments in 2011 and 2012 showed that B. eleusines conidial agent displayed good activity on barnyardgrass, monochoria [Monochoria vaginalis (Burm.f.) Presl. Ex Kunth.], and small-flower umbrella sedge (Cyperus difformis L.) and had no negative impact on the rice plant. It also reduced the loss of rice yield when compared with the non-treated control and could make this pathogen a conidial agent for commercial bioherbicidal development in the future.

Echinochloa crus-galli (L.) P. Beauv (common name: barnyard grass) is a major weed in rice-growing areas and has evolved resistance to multiple herbicides. Florpyrauxifen-benzyl (trade name Rinskor) is a novel synthetic auxin herbicide that was approved in China in 2017 and is widely used in rice production to control resistant weeds, including barnyard grass. We identified a florpyrauxifen-benzyl-resistant E. crus-galli biotype with a resistance index (RI) of 11.89 using screen house herbicide experiments. To understand the phytotoxicity mechanisms of florpyrauxifen-benzyl, we used transcriptomics technologies to compare the gene expression profiles of florpyrauxifen-benzyl treatment on phytohormone transduction between florpyrauxifen-benzyl-resistant and -susceptible barnyard grasses (Echinochloa crus-galli (L.) P. Beauv). A total of 1810 DEGs were identified in the S comparison setting (FTS vs. UTS), and 915 DEGs were identified in the R comparison setting (FTR vs. UTR); 464 genes overlapped between the two comparison groups. Approximately sixty-nine hormone-related DEGs were detected after treatment with florpyrauxifen-benzyl in both R and S biotypes. At 24 h after florpyrauxifen-benzyl treatment, compared with the R biotype, the S biotype showed a stronger auxin response and higher expression of related genes involved in ethylene and abscisic acid biosynthesis and signal transduction. In addition, a brassinolide receptor gene was upregulated after florpyrauxifen-benzyl treatment and had higher expression in the S biotype than in the R biotype. This study is the first transcriptome analysis of the differential effects of florpyrauxifen-benzyl treatment between florpyrauxifen-benzyl-resistant and -susceptible E. crus-galli. It reflects the difference in phytohormone biosynthesis and signal transduction between R and S barnyard grasses in response to florpyrauxifen-benzyl treatment and will be helpful for understanding the phytotoxicity mechanisms of florpyrauxifen-benzyl.

Roegneria kamoji Ohwi (Poaceae), a wild relative plant of wheat which is widely distributed across China, has become a dominant and problematic weed in wheat fields in some regions. We have previously confirmed that R. kamoji is highly tolerant to foliar-applied acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors in wheat (Triticum aestivum L.). The sensitivity of R. kamoji to pre-emergence (PRE) herbicides and the basis of fenclorim increase selectivity to butachlor between wheat and R. kamoji were evaluated in this study. Screenhouse bioassay showed that R. kamoji exhibited similar sensitivity to wheat to PRE herbicides at their recommended field doses (RFD); it also showed that buatchlor provides the highest relative control for R. kamoji (53.4% emergence and 81.5% fresh weight reduction, respectively), while it had no impact on seedling emergence of wheat among the six PRE herbicides. When butachlor was applied at four-fold RFD, no R. kamoji seedlings emerged; however, it significantly reduced the above-ground biomass of wheat compared with the non-treated control. Pre-treatment with herbicide safener fenclorim by seed soaking increased the ED₁₀ value of butachlor to wheat from 221.8 to 1600.1 g a.i. ha⁻¹, thus increasing the selectivity index from 9.6 to 68.9 between wheat and R. kamoji. The activities of α-amylase activity and protein content during germination, and glutathione-S-transferase (GST) and β-ketoacyl-CoA synthase (KCS) in the seedlings, could be induced by butachlor in both wheat seeds with or without fenclorim pre-soaking. These results suggested that butachlor provides the highest control for R. kamoji and did not affect germination and emergence in wheat. The basis of fenclorim-increased selectivity to butachlor was associated with the induced GST and KCS-mediated enhanced herbicide metabolism in wheat.