Combination of surface enhanced Raman scattering and multivariate statistical method allows to identify and distinguish three subtypes of non-small-cell lung cancer cells and leukocytes on the single-cell level.

Melanoma is a malignant tumor formed by the cancerous transformation of melanocytes, and its medical images contain much information. However, the percentage of the critical information in the image is small, and the noise is non-uniformly distributed. We propose a new multi-threshold image segmentation model based on the two-dimensional histogram approach to the above problem. We present an enhanced ant colony optimization for continuous domains (EACOR) in the proposed model based on the soft besiege and chase strategies. Further, EACOR is combined with two-dimensional Kapur’s entropy to search for the optimal thresholds. An experiment on the IEEE CEC2014 benchmark function was conducted to measure the reliable global search capability of the EACOR algorithm in the proposed model. Moreover, we have also conducted several sets of experiments to test the validity of the image segmentation model proposed in this paper. The experimental results show that the segmented images from the proposed model outperform the comparison method in several evaluation metrics. Ultimately, the model proposed in this paper can provide high-quality samples for subsequent analysis of melanoma pathology images.