In a pot culture experiment, five different species of Brassica (Brassica juncea, Brassica campestris, Brassica carinata, Brassica napus, and Brassica nigra) were grown for screening possible accumulators of heavy metals, viz. Zn, Cu, Ni, and Pb. The plants were grown to maturity in a soil irrigated with sewage effluents for more than two decades in West Delhi, India. The soil analysis showed enhanced accumulation of Zn, Cu, Ni, and Pb in this sewage-irrigated soil. Among all species, B. carinata showed the highest concentration (mg kg⁻¹) as well as uptake (μg pot⁻¹) of Ni and Pb at maturity. Although B. campestris showed a higher concentration of Zn in its shoots (stem plus leaf), B. carinata extracted the largest amount of this metal due to greater biomass production. However, B. juncea phytoextracted the largest amount of Cu from the soil. In general, the highest concentration and uptake of metal was observed in shoots compared to roots or seeds of the different species. Among the Brassica spp., B. carinata cv. DLSC1 emerged as the most promising, showing greater uptake of Zn, Ni, and Pb, while B. juncea cv. Pusa Bold showed the highest uptake of Cu. The B. napus also showed promise, as it ranked second with respect to total uptake of Pb, Zn, and Ni, and third for Cu. Total uptake of metals by Brassica spp. correlated negatively with available as well as the total soil metal concentrations. Among the root parameters, root length emerged as the powerful parameter to dictate the uptake of metals by Brassica spp. Probably for the first time, B. carinata was reported as a promising phytoextractor for Zn, Ni, and Pb, which performed better than B. juncea.

Clay–organic complexes (COC) impart chemical and physical protection to soil carbon (C). In the present study, the aim was to assess the long-term effects of different organic amendments on C stability in COC, distribution of the aggregates, C concentration in the aggregates and labile organic C fractions in the inceptisols located at the research farm situated in the semi-arid sub-tropical climate of India. The results showed that the COC, the percentage of large macroaggregates (LMA) (>2 mm) and the C associated with the aggregates decreased through the soil depths, whereas the other size fractions of soil aggregates (<2 mm) showed an increasing trend. The COC was significantly higher (4.4 times) in soil treatments where farmyard manures (FYM), green manure (GM) and biofertilizers (BF) were applied together (FYM + GM + BF). The organic amendments increased the proportion of LMA over control (no application of organic amendments) to a magnitude of 83 to 101% and the C associated with LMA to a magnitude of 0.48 to 9.8% over control in surface soil. On average, the combined application of FYM, GM and BF exhibited higher C accumulation in almost all soil aggregate fractions, except microaggregates (mA), i.e., (0.25–0.053 mm) size fractions, where application of FYM alone recorded the highest value. Averaged over soil depths, the particulate organic C (POC), dissolved organic C (DOC) and potassium permanganate oxidizable C (POXC) were significantly higher under integrated organic treatments than individual amendments. The significantly higher correlation between COC and the percentage of LMA and aggregate-associated C implied their direct role in soil aggregate formation and their stability. The carbon stability, i.e., retention time of humus in soil, was significantly correlated with soil organic carbon, dissolved organic carbon, particulate organic carbon, potassium permanganate oxidizable C, C mineralization (C_min), glomalin-related soil protein (GRSP), macroaggregate (MA)-associated C and rice equivalent yield (REY). Overall, the data suggest that the combined application of FYM + GM + BF promotes soil quality under rice–wheat rotation in inceptisols in semi-arid sub-tropical India.

Land use changes, especially the conversion of native forest vegetation to cropland and plantations in tropical regions, can potentially alter soil C dynamics. A study was conducted to assess the effects of various land uses and soil managements (agro-forestry plantation, vegetable field, tube-well irrigated rice–wheat, sewage-irrigated rice–wheat, and uncultivated soils) on soil pH, bulk density, soil organic C (SOC), particulate organic C (POC), microbial biomass C (MBC), C mineralisation (Cmin), microbial quotient, and microbial metabolic quotient (qCO2) in 0−0.05, 0.05−0.10, and 0.10−0.20 m soil depths. At 0−0.05 m, the bulk density was lowest (1.29 Mg/m3) in agro-forestry soil, whereas the uncultivated soil (jointly with vegetable field soil) showed highest bulk density (1.48 Mg/m3). Sewage-irrigated rice–wheat soil showed lowest pH particularly in the 0−0.05 and 0.10−0.20 m soil layer. Irrespective of soil depths, agro-forestry plantation showed greater SOC followed by sewage-irrigated rice–wheat soil. Nevertheless, agro-forestry soil also showed highest stock of SOC (33.7 Mg/ha), POC (3.58 Mg/ha), and MBC (0.81 Mg/ha) in the 0−0.20 m soil layer. Sewage-irrigated rice–wheat jointly with agro-forestry soil showed greatest Cmin in the 0−0.20 m soil layer, although the former supported lower SOC stock. The decrease in SOC (SOC0−0.05 m/SOC0.10−0.20 m) and Cmin (Cmin 0−0.05 m/Cmin 0.10−0.20 m) along soil depth was significantly higher in the agro-forestry system than in most of the other land use and soil management systems. Microbial quotient was highest in sewage-irrigated rice–wheat soil, particularly in the 0−0.05 m soil depth, whereas qCO2 was greater in uncultivated soil. In general, microbial quotients decreased, whereas qCO2 increased down the soil profile.