The xyloglucan endotransglucosylase/hydrolase (XET/XEH, also named XTH) family is a multigene family, the function of which plays a significant role in cell-wall rebuilding and stress tolerance in plants. However, the specific traits of the XTH gene family members and their expression pattern in different tissues and under stress have not been carried out in sweet potato. Thirty-six XTH genes were identified in I. batatas, all of which had conserved structures (Glyco_hydro_16). Based on Neighbor-Joining phylogenetic analysis the IbXTHs can be divided into three subfamilies—the I/II, IIIA, and IIIB subfamilies, which were unevenly distributed on 13 chromosomes, with the exception of Chr9 and Chr15. Multiple cis-acting regions related to growth and development, as well as stress responses, may be found in the IbXTH gene promoters. The segmental duplication occurrences greatly aided the evolution of IbXTHs. The results of a collinearity analysis showed that the XTH genes of sweet potato shared evolutionary history with three additional species, including A. thaliana, G. max, and O. sativa. Additionally, based on the transcriptome sequencing data, the results revealed that the IbXTHs have different expression patterns in leaves, stems, the root body (RB), the distal end (DE), the root stock (RS), the proximal end (PE), the initiative storage root (ISR), and the fibrous root (FR), and many of them are well expressed in the roots. Differentially expressed gene (DEG) analysis of FRs after hormone treatment of the roots indicated that IbXTH28 and IbXTH30 are up-regulated under salicylic acid (SA) treatment but down-regulated under methyl jasmonate (MeJA) treatment. Attentionally, there were only two genes showing down-regulation under the cold and drought treatment. Collectively, all of the findings suggested that genes from the XTH family are crucial for root specificity. This study could provide a theoretical basis for further research on the molecular function of sweet potato XTH genes.

Heat treatment is a widely applied technique in the preservation of fruits and vegetables, effectively addressing issues such as disease management, rot prevention, and browning. In this study, we investigated the impact of heat treatment at 35 °C for 24 h on the quality characteristics and disease resistance of two sweet potato varieties, P32/P (Ipomoea batatas (L.) Lam. cv ‘Pushu13’) and Xinxiang (Ipomoea batatas (L.) Lam. cv ‘Xinxiang’). The growth in vitro and reproduction of Rhizopus stolonifer were significantly inhibited at 35 °C. However, it resumed when returned to suitable growth conditions. The heat treatment (at 35 °C for 24 h) was found to mitigate nutrient loss during storage while enhancing the structural characteristics and free radical scavenging capacity of sweet potato. Additionally, it led to increased enzyme activities for APX, PPO, and POD, alongside decreased activities for Cx and PG, thereby enhancing the disease resistance of sweet potato against soft rot. As a result, the heat treatment provided a theoretical basis for the prevention of sweet potato soft rot and had guiding significance for improving the resistance against sweet potato soft rot.

With the rapid growth of plastic production, plastic pollution has become increasingly widespread and persistent in the environment. This persistent pollution poses potential risks to ecosystems and human health. Microbial biodegradation offers a promising approach to plastic waste management because it operates under mild reaction conditions and has potential for sustainable application. This review summarizes recent advances in the major microbial groups involved in plastic biodegradation, methods for assessing degradation efficiency, underlying mechanisms, and strategies for improving degradation performance. Assessment methods are discussed, from physicochemical characterization to biochemical validation. Meanwhile, it emphasizes that real plastic biodegradation and degradation efficiency require multiple complementary lines of evidence to judge in a more complete and objective way. The complete process and molecular mechanisms of microbial plastic degradation are then described, including microbial attachment and biofilm formation, enzymatic degradation, intracellular metabolism, and mineralization of degradation products. In addition, it summarizes various efficiency optimization strategies for degradation, including plastic pretreatment, bioaugmentation, and regulation of environmental parameters. The lack of standardized assessment, limited practical translation, and ecological risks are the identified key challenges. Finally, standardized efficiency evaluation strategies, plastic upcycling, and emerging technologies for subsequent development were discussed. This review aims to provide a reference for understanding microbial plastic degradation.