Resistance training adaptively increases the muscle strength associated with protein anabolism. Previously, we showed that the combined intake of astaxanthin, β-carotene, and resveratrol can accelerate protein anabolism in the skeletal muscle of mice. The purpose of this study was to investigate the effect of anabolic nutrient-rich foods on muscle adaptation induced by resistance training. Twenty-six healthy men were divided into control and intervention groups. All participants underwent a resistance training program twice a week for 10 weeks. Astaxanthin-, β-carotene-, and resveratrol-rich foods were provided to the intervention group. Body composition, nutrient intake, maximal voluntary contraction of leg extension, oxygen consumption, and serum carbonylated protein level were measured before and after training. The skeletal muscle mass was higher after training than before training in both groups (p < 0.05). Maximal voluntary contraction was increased after training in the intervention group (p < 0.05), but not significantly increased in the control group. Resting oxygen consumption was higher after training in the intervention group only (p < 0.05). As an oxidative stress marker, serum carbonylated protein level tended to be lower immediately after exercise than before exercise in the intervention group only (p = 0.056). Intake of astaxanthin-, β-carotene-, and resveratrol-rich foods supported resistance training-induced strength and metabolic adaptations.

This study shows the effect of omega-3 unsaturated fatty acids via perilla oil intake on gut microbiota and constipation. Eight sedentary females participated in a nonrandomized placebo-controlled pilot study consisting of eight-week perilla oil (OIL) and placebo (PLA) intervention phases. There was a 10-month washout period between phases. All participants received 9 g of perilla oil-containing jelly in the OIL phase, and a placebo jelly in the PLA phase. Gut microbiota, α-diversity, and constipation scores were measured pre- and post-intervention in both phases. The α-diversity, an important indicator of gut microbiota diversity, was significantly increased post-intervention (4.5 ± 0.1) compared to pre-intervention (3.8 ± 0.3) in the OIL only (p = 0.021). Notably, the level of α-diversity was maintained even after the washout period of 10 months. Butyrate-producing bacteria, Lachnospiraceae (%), did not change in the OIL but were significantly reduced post-intervention (15.1 ± 4.8) compared to pre-intervention (20.1 ± 7.0) in the PLA (p = 0.040). In addition, the constipation scores were significantly or tended to be reduced during the OIL phase only (p < 0.05, p < 0.1). In conclusion, an eight-week perilla oil supplementation may enhance and establish the diversity of gut microbiota, which may relieve constipation.

To improve endurance performance, intake of n-3 fatty acid has been investigated, but studies on athletes have been limited. The aim of this study was to investigate the effects of EPA and DHA intake for 24 months on blood fatty acid changes in female long-distance athletes. Twelve subjects took EPA 664 mg and DHA 284 mg daily for 24 months, and blood tests were conducted once a month. It was found that the EPA concentration increased by 126％ (p ＜ 0.01) after 3 months, whereas the AA concentration decreased by 17％ (p ＜ 0.05) a month after the start of the intervention. The EPA/AA ratio was increased by 110％ (p ＜ 0.01) after 3 months (0.86 ± 0.05) compared to that before the intervention (0.41 ± 0.04) and then shifted within the range 0.67‐0.98 (p ＜ 0.05 and p ＜ 0.01). On the other hand, there was no change in the blood levels of DHA. Therefore, it was suggested that daily intake of EPA and DHA may increase the EPA/AA ratio in female long-distance athletes.