A recent study reported an association between inactivated influenza vaccine (IIV) and spontaneous abortion (SAB), but only among women who had also been vaccinated in the previous influenza season. We sought to estimate the association between IIV administered in three recent influenza seasons and SAB among women who were and were not vaccinated in the previous influenza season. We conducted a case-control study over three influenza seasons (2012-13, 2013-14, 2014-15) in the Vaccine Safety Datalink (VSD). Cases (women with SAB) and controls (women with live births) were matched on VSD site, date of last menstrual period, age group, and influenza vaccination status in the previous influenza season. Of 1908 presumptive cases identified from the electronic record, 1236 were included in the main analysis. Administration of IIV was documented in several risk windows, including 1-28, 29-56, and >56 days before the SAB date. Among 627 matched pairs vaccinated in the previous season, no association was found between vaccination in the 1-28 day risk window and SAB (adjusted odds ratio (aOR) 0.9; 95% confidence interval (CI) 0.6-1.5). The season-specific aOR ranged from 0.5 to 1.7 with all CIs including the null value of 1.0. Similarly, no association was found among women who were not vaccinated in the previous season; the season-specific aOR in the 1-28 day risk window ranged from 0.6 to 0.7 and the 95% CI included 1.0 in each season. There was no association found between SAB and influenza vaccination in the other risk windows, or when vaccine receipt was analyzed relative to date of conception. During these seasons we found no association between IIV and SAB, including among women vaccinated in the previous season. These findings lend support to current recommendations for influenza vaccination at any time during pregnancy, including the first trimester.

Manure application influences ammonia (NH₃) and greenhouse gas emissions; however, few studies have quantified the effects of manure application methods and timing on NH₃, nitrous oxide (N₂O), carbon dioxide (CO₂), and methane (CH₄) fluxes simultaneously. We evaluated surface-applied liquid manure application with disk incorporation versus injection on NH₃, N₂O, CO₂, and CH₄ fluxes in central Wisconsin corn silage (Zea mays L.) plots during pre-plant (PP) and sidedress (SD) application windows from 2009 to 2011. Manure treatments were PP injection (PP-Inject) and injection at sidedress time (SD-Inject) to growing corn, along with two incorporation times for PP surface application (within 24 h—PP-1-hr; within 3 days—PP-3-day). Mean NH₃ emissions were 95% lower for injected treatments compared to surface application in both years, with larger losses for PP-3-day and SD-Surf. While N₂O fluxes were generally low, larger increases after manure application were associated with injection and triggered by soil moisture/temperature changes. Mean CO₂ and CH₄ were unaffected by manure treatments and influenced more by weather. Overall, injection conserved more available soil N while contributing to modest N₂O emission, suggesting manure injection may offer greater agri-environmental benefits on the whole over surface application.