The present work describes the chemical preparation of Schiff bases derived from 4,4′-diaminodiphenyl sulfone (L₁–L₅) and their Co(II) metal complexes. The evaluation of antimicrobial and anticancer activities against MCF-7 cell line and human lung cancer cell line A-549 was performed. The aforementioned synthesized compounds are characterized by spectroscopic techniques and elemental analysis confirms successful synthesis. The results from the above analytical techniques revealed that the complexes are in an octahedral geometry. The antimicrobial activity of the synthesized Schiff base ligands and their metal complexes under study was carried out by using the agar well diffusion method. The ligand and complex interactions for biological targets were predicted using molecular docking and high binding affinities. Further, the anticancer properties of the synthesized compounds are performed against the MCF-7 cell line and human lung cancer cell line A-549 using adriamycin as the standard drug.

Bacteria and fungi produce polymers of carbohydrates called exopolysaccharides outside of their cell walls. Because of their significant osmotic resilience, FreshWater bacteria synthesise exopolysaccharides at low salt concentrations. This study focuses on the exopolysaccharide-producing bacteria isolated from the lake and pond environment in Dharashiv, Maharashtra. Freshwater samples were collected from the Ruihar Lake, the industrial area pond, the sub-campus area ponds, and the Upla Dam of Dharashiv. Isolation of freshwater bacteria was carried out by using the serial dilution method and analysed for their ability to synthesise exopolysaccharide (EPS). A variety of screening techniques, Congo red agar plate, and the String test, were employed to identify and evaluate the exopolysaccharide (EPS) production by the isolated freshwater bacteria. Our study revealed that, after screening, eight out of the thirteen isolates demonstrated potential as promising strains for exopolysaccharide (EPS) synthesis. Out of eight selected isolates, after production and optimization, one isolate produced the maximum EPS of 0.050mg/50ml. The bacterial strains were identified by using 16S rRNA sequencing, and the organism was found to be Bacillus subtilis. After extraction, the EPS was purified by membrane dialysis and anion exchange chromatography. Purified EPS was confirmed by using FT-IR and GC-MS analysis. The purified EPS was subsequently evaluated for its antimicrobial activities.