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Prof. Vittorio Sambri
Professor of Microbiology, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy AND Unit of Microbiology - The Great Romagna Hub Laboratory -Pievesestina (FC), Italy

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0 Molecular diagnosis
0 chikungunya
0 Treponema pallidum
0 west nile virus
0 usutu virus

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west nile virus
chikungunya
Treponema pallidum
usutu virus
Spirochetes
Borrelia spp.
Molecular diagnosis

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Review
Published: 13 August 2021 in Nutrients
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Steatohepatitis and hepatobiliary manifestations constitute some of the most common extra-intestinal manifestations of Inflammatory Bowel Disease (IBD). On the other hand, non-alcoholic fatty liver disease (NAFLD) affects around 25% of the world’s population and is attracting ever more attention in liver transplant programs. To outline the specific pathways linking these two conditions is a pressing task for 21st-century researchers. We are accustomed to expecting the occurrence of fatty liver disease in obese people, but current evidence suggests that there are several different pathways also occurring in underweight patients. Genetic factors, inflammatory signals and microbiota are key players that could help in understanding the entire pathogenesis of NAFLD, with the aim of defining the multiple expressions of malnutrition. In the current review, we summarize the most recent literature regarding the epidemiology, pathogenesis and future directions for the management of NAFLD in patients affected by IBD.

ACS Style

Giulia Gibiino; Alessandro Sartini; Stefano Gitto; Cecilia Binda; Monica Sbrancia; Chiara Coluccio; Vittorio Sambri; Carlo Fabbri. The Other Side of Malnutrition in Inflammatory Bowel Disease (IBD): Non-Alcoholic Fatty Liver Disease. Nutrients 2021, 13, 2772 .

AMA Style

Giulia Gibiino, Alessandro Sartini, Stefano Gitto, Cecilia Binda, Monica Sbrancia, Chiara Coluccio, Vittorio Sambri, Carlo Fabbri. The Other Side of Malnutrition in Inflammatory Bowel Disease (IBD): Non-Alcoholic Fatty Liver Disease. Nutrients. 2021; 13 (8):2772.

Chicago/Turabian Style

Giulia Gibiino; Alessandro Sartini; Stefano Gitto; Cecilia Binda; Monica Sbrancia; Chiara Coluccio; Vittorio Sambri; Carlo Fabbri. 2021. "The Other Side of Malnutrition in Inflammatory Bowel Disease (IBD): Non-Alcoholic Fatty Liver Disease." Nutrients 13, no. 8: 2772.

Review
Published: 23 June 2021 in International Journal of Molecular Sciences
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Diet is the first to affect our intestinal microbiota and therefore the state of eubiosis. Several studies are highlighting the potential benefits of taking certain nutritional supplements, but a dietary regime that can ensure the health of the intestinal microbiota, and the many pathways it governs, is not yet clearly defined. We performed a systematic review of the main studies concerning the impact of an omnivorous diet on the composition of the microbiota and the production of short-chain fatty acids (SCFAs). Some genera and phyla of interest emerged significantly and about half of the studies evaluated consider them to have an equally significant impact on the production of SCFAs, to be a source of nutrition for our colon cells, and many other processes. Although numerous randomized trials are still needed, the Mediterranean diet could play a valuable role in ensuring our health through direct interaction with our microbiota.

ACS Style

Giulia Gibiino; Martina De Siena; Monica Sbrancia; Cecilia Binda; Vittorio Sambri; Antonio Gasbarrini; Carlo Fabbri. Dietary Habits and Gut Microbiota in Healthy Adults: Focusing on the Right Diet. A Systematic Review. International Journal of Molecular Sciences 2021, 22, 6728 .

AMA Style

Giulia Gibiino, Martina De Siena, Monica Sbrancia, Cecilia Binda, Vittorio Sambri, Antonio Gasbarrini, Carlo Fabbri. Dietary Habits and Gut Microbiota in Healthy Adults: Focusing on the Right Diet. A Systematic Review. International Journal of Molecular Sciences. 2021; 22 (13):6728.

Chicago/Turabian Style

Giulia Gibiino; Martina De Siena; Monica Sbrancia; Cecilia Binda; Vittorio Sambri; Antonio Gasbarrini; Carlo Fabbri. 2021. "Dietary Habits and Gut Microbiota in Healthy Adults: Focusing on the Right Diet. A Systematic Review." International Journal of Molecular Sciences 22, no. 13: 6728.

Journal article
Published: 28 May 2021 in Viruses
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in Wuhan, China, in late 2019 and is the causative agent of the coronavirus disease 2019 (COVID-19) pandemic. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) represents the gold standard for diagnostic assays even if it cannot precisely quantify viral RNA copies. Thus, we decided to compare qRT-PCR with digital polymerase chain reaction (dPCR), which is able to give an accurate number of RNA copies that can be found in a specimen. However, the aforementioned methods are not capable to discriminate if the detected RNA is infectious or not. For this purpose, it is necessary to perform an endpoint titration on cell cultures, which is largely used in the research field and provides a tissue culture infecting dose per mL (TCID50/mL) value. Both research and diagnostics call for a model that allows the comparison between the results obtained employing different analytical methods. The aim of this study is to define a comparison among two qRT-PCR protocols (one with preliminary RNA extraction and purification and an extraction-free qRT-PCR), a dPCR and a titration on cell cultures. The resulting correlations yield a faithful estimation of the total number of RNA copies and of the infectious viral burden from a Ct value obtained with diagnostic routine tests. All these estimations take into consideration methodological errors linked to the qRT-PCR, dPCR and titration assays.

ACS Style

Martina Brandolini; Francesca Taddei; Maria Marino; Laura Grumiro; Agata Scalcione; Maria Turba; Fabio Gentilini; Michela Fantini; Silvia Zannoli; Giorgio Dirani; Vittorio Sambri. Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management. Viruses 2021, 13, 1022 .

AMA Style

Martina Brandolini, Francesca Taddei, Maria Marino, Laura Grumiro, Agata Scalcione, Maria Turba, Fabio Gentilini, Michela Fantini, Silvia Zannoli, Giorgio Dirani, Vittorio Sambri. Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management. Viruses. 2021; 13 (6):1022.

Chicago/Turabian Style

Martina Brandolini; Francesca Taddei; Maria Marino; Laura Grumiro; Agata Scalcione; Maria Turba; Fabio Gentilini; Michela Fantini; Silvia Zannoli; Giorgio Dirani; Vittorio Sambri. 2021. "Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management." Viruses 13, no. 6: 1022.

Journal article
Published: 04 May 2021 in Applied Sciences
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The accidental contamination of food and food packaging surfaces with SARS-CoV-2 is of increasing concern among scientists and consumers, particularly in relation to fresh foods that are consumed without further cooking. The use of chemical sanitizers is often not suitable for these kinds of commodities; therefore, a non-thermal sanitation technology could help to increase safety in relation to the food supply chain. Cold plasma has proven to be a promising strategy for virus inactivation. This research is aimed at evaluating the ability of a cold plasma sanitation system to inactivate SARS-CoV-2 RNA on packaged foods. Two different plastic materials were investigated and subjected to 5- and 10-min exposure to plasma after experimental inoculum of the RNA. In addition to viral degradation, possible changes in the performance of the materials were evaluated. Shelf-life of the foods, after exposure of the packages to plasma, was also investigated. Results showed that 10 min of exposure was sufficient to almost completely eliminate the viral RNA from package surfaces. The treatment did not produce any significant variation in packaging material performance or the shelf-life of the tested packaged products, indicating the potentiality of this treatment for the decontamination of packaged products.

ACS Style

Filippo Capelli; Silvia Tappi; Tommaso Gritti; Ana De Aguiar Saldanha Pinheiro; Romolo Laurita; Urszula Tylewicz; Francesco Spataro; Giacomo Braschi; Rosalba Lanciotti; Federico Gómez Galindo; Valentina Siracusa; Santina Romani; Matteo Gherardi; Vittorio Colombo; Vittorio Sambri; Pietro Rocculi. Decontamination of Food Packages from SARS-CoV-2 RNA with a Cold Plasma-Assisted System. Applied Sciences 2021, 11, 4177 .

AMA Style

Filippo Capelli, Silvia Tappi, Tommaso Gritti, Ana De Aguiar Saldanha Pinheiro, Romolo Laurita, Urszula Tylewicz, Francesco Spataro, Giacomo Braschi, Rosalba Lanciotti, Federico Gómez Galindo, Valentina Siracusa, Santina Romani, Matteo Gherardi, Vittorio Colombo, Vittorio Sambri, Pietro Rocculi. Decontamination of Food Packages from SARS-CoV-2 RNA with a Cold Plasma-Assisted System. Applied Sciences. 2021; 11 (9):4177.

Chicago/Turabian Style

Filippo Capelli; Silvia Tappi; Tommaso Gritti; Ana De Aguiar Saldanha Pinheiro; Romolo Laurita; Urszula Tylewicz; Francesco Spataro; Giacomo Braschi; Rosalba Lanciotti; Federico Gómez Galindo; Valentina Siracusa; Santina Romani; Matteo Gherardi; Vittorio Colombo; Vittorio Sambri; Pietro Rocculi. 2021. "Decontamination of Food Packages from SARS-CoV-2 RNA with a Cold Plasma-Assisted System." Applied Sciences 11, no. 9: 4177.

Journal article
Published: 01 December 2020 in International Journal of Environmental Research and Public Health
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Dental-care workers operate very close to the patient’s mouth and are at high risk of contamination by SARS-CoV-2. Droplets may be contaminated by patient’s saliva and exhaled breath particles. All asymptomatic patients should be considered as Coronavirus positive. All dental procedures must be revised after positive identification of SARS-Cov-2. Novel recommendations as the use of novel suction cannula designed for fast spray/saliva aspiration, use of Tyvek suits and innovative sprayhoods designed for dental-care worker protections are proposed to prevent virus transmission. New tailored operative and clinical procedures are being currently developed by university dental clinics and hospitals in attempt to reduce risk for dental workers and patients.

ACS Style

Maria Giovanna Gandolfi; Fausto Zamparini; Andrea Spinelli; Vittorio Sambri; Carlo Prati. Risks of Aerosol Contamination in Dental Procedures during the Second Wave of COVID-19—Experience and Proposals of Innovative IP&C in Dental Practice. International Journal of Environmental Research and Public Health 2020, 17, 8954 .

AMA Style

Maria Giovanna Gandolfi, Fausto Zamparini, Andrea Spinelli, Vittorio Sambri, Carlo Prati. Risks of Aerosol Contamination in Dental Procedures during the Second Wave of COVID-19—Experience and Proposals of Innovative IP&C in Dental Practice. International Journal of Environmental Research and Public Health. 2020; 17 (23):8954.

Chicago/Turabian Style

Maria Giovanna Gandolfi; Fausto Zamparini; Andrea Spinelli; Vittorio Sambri; Carlo Prati. 2020. "Risks of Aerosol Contamination in Dental Procedures during the Second Wave of COVID-19—Experience and Proposals of Innovative IP&C in Dental Practice." International Journal of Environmental Research and Public Health 17, no. 23: 8954.

Communication
Published: 31 August 2020 in Plasma Processes and Polymers
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One of the major concerns in the COVID‐19 pandemic is related to the possible transmission in poorly ventilated spaces of SARS‐CoV‐2 through aerosol microdroplets, which can remain in the air for long periods of time and be transmitted to others over distances >1 m. Cold atmospheric pressure plasmas can represent a promising solution, thanks to their ability in producing a blend of many reactive species, which can inactivate the airborne aerosolized microorganisms. In this study, a dielectric barrier discharge plasma source is used to directly inactivate suitably produced bioaerosols containing Staphylococcus epidermidis or purified SARS‐CoV‐2 RNA flowing through it. Results show that for low residence times (<0.2 s) in the plasma region a 3.7 log R on bacterial bioaerosol and degradation of viral RNA can be achieved.

ACS Style

Alina Bisag; Pasquale Isabelli; Romolo Laurita; Cristiana Bucci; Filippo Capelli; Giorgio Dirani; Matteo Gherardi; Giulia Laghi; Alessandro Paglianti; Vittorio Sambri; Vittorio Colombo. Cold atmospheric plasma inactivation of aerosolized microdroplets containing bacteria and purified SARS‐CoV‐2 RNA to contrast airborne indoor transmission. Plasma Processes and Polymers 2020, 17, 1 .

AMA Style

Alina Bisag, Pasquale Isabelli, Romolo Laurita, Cristiana Bucci, Filippo Capelli, Giorgio Dirani, Matteo Gherardi, Giulia Laghi, Alessandro Paglianti, Vittorio Sambri, Vittorio Colombo. Cold atmospheric plasma inactivation of aerosolized microdroplets containing bacteria and purified SARS‐CoV‐2 RNA to contrast airborne indoor transmission. Plasma Processes and Polymers. 2020; 17 (10):1.

Chicago/Turabian Style

Alina Bisag; Pasquale Isabelli; Romolo Laurita; Cristiana Bucci; Filippo Capelli; Giorgio Dirani; Matteo Gherardi; Giulia Laghi; Alessandro Paglianti; Vittorio Sambri; Vittorio Colombo. 2020. "Cold atmospheric plasma inactivation of aerosolized microdroplets containing bacteria and purified SARS‐CoV‐2 RNA to contrast airborne indoor transmission." Plasma Processes and Polymers 17, no. 10: 1.

Other
Published: 13 August 2020
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Background Pathogenesis of Coronavirus disease 2019 (Covid-19) is poorly understood. Most histologic studies come from post-mortem analysis, with existing data indicating that histologic features of acute respiratory distress syndrome are typically present in fatal cases. However, this observation may be misleading, due to confounding factors in pre-terminal disease, including injury resulting from prolonged mechanical ventilation. Ante-mortem lung biopsy may provide major pathogenetic insights, potentially providing a basis for novel treatment approaches. Aim This comparative, multicenter, prospective, observational study was planned to identify ante-mortem histological profile and immunohistochemical features of lung tissue in patients with Covid-19 in early and late phases of the disease, including markers of inflammatory cells and major pathways involved in the cytokine storm triggering. Methods Enrolled patients underwent lung biopsy, according to the study protocol approved by local Ethical Committee, either within 15 days of the first symptoms appearing (early phase) or after >15 days (more advanced disease). Key exclusion criteria were excessive or uncorrectable bleeding risk and cardiovascular disease with heart failure. Lung samples were obtained by conventional transbronchial biopsy, trans-bronchial lung cryobiopsy or surgical lung biopsy. Results 23 patients were enrolled: 12 patients underwent lung biopsy within 15 days and 11 patients more than 15 days after the onset of symptoms. Early biopsies were characterized by spots of patchy acute lung injury (ALI) with alveolar type II cells hyperplasia and significant vascular abnormalities (disordered angiogenesis with alveolar capillary hyperplasia, luminal enlargement and thickened walls of pulmonary venules, perivascular CD4-T-cell infiltration), with no hyaline membranes. In the later stages, the alveolar architecture appeared disrupted, with areas of organizing ALI, venular congestion and capillary thromboembolic microangiopathy. Striking phenotypic features were demonstrated in hyperplastic pneumocytes and endothelial cells, including the expression of phospho-STAT3 and molecules involved in immunoinhibitory signals (PD-L1 and IDO-1). Alveolar macrophages exhibited macrophage-related markers (CD68, CD11c, CD14) together with unusual markers, such as DC-Lamp/CD208, CD206, CD123/IL3AR. Conclusion A morphologically distinct “Covid pattern” was identified in the earlier stages of the disease, with prominent epithelial and endothelial cell abnormalities, that may be potentially reversible, differing strikingly from findings in classical diffuse alveolar damage. These observations may have major therapeutic implications, justifying studies of early interventions aimed at mitigating inflammatory organ injury.

ACS Style

Doglioni Claudio; Ravaglia Claudia; Rossi Giulio; Dubini Alessandra; Pedica Federica; Piciucchi Sara; Vizzuso Antonio; Pecciarini Lorenza; Stella Franco; Maitan Stefano; Agnoletti Vanni; Gamberini Emiliano; Russo Emanuele; Puglisi Silvia; Arcadu Antonella; Donati Luca; Di Cesare Simona; Grosso Carmela; Poletti Giovanni; Sambri Vittorio; Fabbri D Elisabetta; Pizzolo Giovanni; Ugel Stefano; Bronte Vincenzo; Wells U Athol; Chilosi Marco; Poletti Venerino. Acute Lung injury evolution in Covid-19. 2020, 1 .

AMA Style

Doglioni Claudio, Ravaglia Claudia, Rossi Giulio, Dubini Alessandra, Pedica Federica, Piciucchi Sara, Vizzuso Antonio, Pecciarini Lorenza, Stella Franco, Maitan Stefano, Agnoletti Vanni, Gamberini Emiliano, Russo Emanuele, Puglisi Silvia, Arcadu Antonella, Donati Luca, Di Cesare Simona, Grosso Carmela, Poletti Giovanni, Sambri Vittorio, Fabbri D Elisabetta, Pizzolo Giovanni, Ugel Stefano, Bronte Vincenzo, Wells U Athol, Chilosi Marco, Poletti Venerino. Acute Lung injury evolution in Covid-19. . 2020; ():1.

Chicago/Turabian Style

Doglioni Claudio; Ravaglia Claudia; Rossi Giulio; Dubini Alessandra; Pedica Federica; Piciucchi Sara; Vizzuso Antonio; Pecciarini Lorenza; Stella Franco; Maitan Stefano; Agnoletti Vanni; Gamberini Emiliano; Russo Emanuele; Puglisi Silvia; Arcadu Antonella; Donati Luca; Di Cesare Simona; Grosso Carmela; Poletti Giovanni; Sambri Vittorio; Fabbri D Elisabetta; Pizzolo Giovanni; Ugel Stefano; Bronte Vincenzo; Wells U Athol; Chilosi Marco; Poletti Venerino. 2020. "Acute Lung injury evolution in Covid-19." , no. : 1.

Chapter
Published: 31 May 2020 in Sexually Transmitted Infections
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The laboratory diagnosis of STDs caused by bacteria and protozoa has been in place since the identification of the specific pathogens. Initially, the culture based techniques and the microscopic examination of genital secretions were considered the reference methods and in selected cases these methods are still relevant to achieve the laboratory diagnosis. In the last decades the major improvements in the diagnostic workflows were made based on the use of recombinant antigens for the serology of syphilis and the implementation of nucleic acids amplification techniques for most of these infections. The laboratory diagnosis of syphilis, Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, and genital mycoplasma is discussed, with a focus on the most innovative approaches.

ACS Style

Vittorio Sambri. The Laboratory Diagnosis of Bacterial Sexually Transmitted Diseases. Sexually Transmitted Infections 2020, 149 -170.

AMA Style

Vittorio Sambri. The Laboratory Diagnosis of Bacterial Sexually Transmitted Diseases. Sexually Transmitted Infections. 2020; ():149-170.

Chicago/Turabian Style

Vittorio Sambri. 2020. "The Laboratory Diagnosis of Bacterial Sexually Transmitted Diseases." Sexually Transmitted Infections , no. : 149-170.

Journal article
Published: 24 March 2020 in Microorganisms
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Lyme disease (LD), caused by infection with Borrelia burgdorferi, is the most common tick-borne infection in many regions of Eurasia. Antibody detection is the most frequently used laboratory test, favoring a two-step serodiagnostic algorithm; immunoenzymatic detection of antibodies to C6 has been shown to perform similarly to a standard two-step workflow. The aim of this study was the performance evaluation of the C6 Lyme ELISA kit compared to a standard two-step algorithm in three laboratories located in the northeastern region of Italy which cater to areas with different LD epidemiology. A total of 804 samples were tested, of which 695 gave concordant results between C6 testing and routine workflow (564 negative, 131 positive). Wherever available, clinical presentation and additional laboratory tests were analyzed to solve discrepancies. The C6 based method showed a good concordance with the standard two-step algorithm (Cohen’s κ = 0.619), however, the distribution of discrepancies seems to point towards a slightly lower specificity of C6 testing, which is supported by literature and could impact on patient management. The C6 ELISA, therefore, is not an ideal stand-alone test; however, if integrated into a two-step algorithm, it might play a part in achieving a sensitive, specific laboratory diagnosis of LD.

ACS Style

Silvia Zannoli; Michela Fantini; Simona Semprini; Barbara Marchini; Barbara Ceccarelli; Monica Sparacino; Pasqua Schiavone; Anna Belgrano; Maurizio Ruscio; Martina Gobbetti; Maira Nicoletti; Eva Robatscher; Elisabetta Pagani; Vittorio Sambri. Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease. Microorganisms 2020, 8, 457 .

AMA Style

Silvia Zannoli, Michela Fantini, Simona Semprini, Barbara Marchini, Barbara Ceccarelli, Monica Sparacino, Pasqua Schiavone, Anna Belgrano, Maurizio Ruscio, Martina Gobbetti, Maira Nicoletti, Eva Robatscher, Elisabetta Pagani, Vittorio Sambri. Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease. Microorganisms. 2020; 8 (3):457.

Chicago/Turabian Style

Silvia Zannoli; Michela Fantini; Simona Semprini; Barbara Marchini; Barbara Ceccarelli; Monica Sparacino; Pasqua Schiavone; Anna Belgrano; Maurizio Ruscio; Martina Gobbetti; Maira Nicoletti; Eva Robatscher; Elisabetta Pagani; Vittorio Sambri. 2020. "Multicenter Evaluation of the C6 Lyme ELISA Kit for the Diagnosis of Lyme Disease." Microorganisms 8, no. 3: 457.

Review
Published: 18 February 2020 in World Journal of Emergency Surgery
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Despite evidence supporting the effectiveness of best practices in infection prevention and management, many healthcare workers fail to implement them and evidence-based practices tend to be underused in routine practice. Prevention and management of infections across the surgical pathway should always focus on collaboration among all healthcare workers sharing knowledge of best practices. To clarify key issues in the prevention and management of infections across the surgical pathway, a multidisciplinary task force of experts convened in Ancona, Italy, on May 31, 2019, for a national meeting. This document represents the executive summary of the final statements approved by the expert panel.

ACS Style

Massimo Sartelli; Leonardo Pagani; Stefania Iannazzo; Maria Luisa Moro; Pierluigi Viale; Angelo Pan; Luca Ansaloni; Federico Coccolini; Marcello Mario D’Errico; Iris Agreiter; Giorgio Amadio Nespola; Francesco Barchiesi; Valeria Benigni; Raffaella Binazzi; Stefano Cappanera; Alessandro Chiodera; Valentina Cola; Daniela Corsi; Francesco Cortese; Massimo Crapis; Francesco Cristini; Alessandro D’Arpino; Belinda De Simone; Stefano Di Bella; Francesco Di Marzo; Abele Donati; Daniele Elisei; Massimo Fantoni; Anna Ferrari; Domitilla Foghetti; Daniela Francisci; Gianni Gattuso; Andrea Giacometti; Guido Cesare Gesuelli; Cristina Marmorale; Enrica Martini; Marcello Meledandri; Rita Murri; Daniela Padrini; Dalia Palmieri; Paola Pauri; Carla Rebagliati; Enrico Ricchizzi; Vittorio Sambri; Anna Maria Schimizzi; Walter Siquini; Loredana Scoccia; Giancarlo Scoppettuolo; Gabriele Sganga; Nadia Storti; Marcello Tavio; Giulio Toccafondi; Fabio Tumietto; Bruno Viaggi; Marco Vivarelli; Cristian Tranà; Melina Raso; Francesco Maria Labricciosa; Sameer Dhingra; Fausto Catena. A proposal for a comprehensive approach to infections across the surgical pathway. World Journal of Emergency Surgery 2020, 15, 1 -26.

AMA Style

Massimo Sartelli, Leonardo Pagani, Stefania Iannazzo, Maria Luisa Moro, Pierluigi Viale, Angelo Pan, Luca Ansaloni, Federico Coccolini, Marcello Mario D’Errico, Iris Agreiter, Giorgio Amadio Nespola, Francesco Barchiesi, Valeria Benigni, Raffaella Binazzi, Stefano Cappanera, Alessandro Chiodera, Valentina Cola, Daniela Corsi, Francesco Cortese, Massimo Crapis, Francesco Cristini, Alessandro D’Arpino, Belinda De Simone, Stefano Di Bella, Francesco Di Marzo, Abele Donati, Daniele Elisei, Massimo Fantoni, Anna Ferrari, Domitilla Foghetti, Daniela Francisci, Gianni Gattuso, Andrea Giacometti, Guido Cesare Gesuelli, Cristina Marmorale, Enrica Martini, Marcello Meledandri, Rita Murri, Daniela Padrini, Dalia Palmieri, Paola Pauri, Carla Rebagliati, Enrico Ricchizzi, Vittorio Sambri, Anna Maria Schimizzi, Walter Siquini, Loredana Scoccia, Giancarlo Scoppettuolo, Gabriele Sganga, Nadia Storti, Marcello Tavio, Giulio Toccafondi, Fabio Tumietto, Bruno Viaggi, Marco Vivarelli, Cristian Tranà, Melina Raso, Francesco Maria Labricciosa, Sameer Dhingra, Fausto Catena. A proposal for a comprehensive approach to infections across the surgical pathway. World Journal of Emergency Surgery. 2020; 15 (1):1-26.

Chicago/Turabian Style

Massimo Sartelli; Leonardo Pagani; Stefania Iannazzo; Maria Luisa Moro; Pierluigi Viale; Angelo Pan; Luca Ansaloni; Federico Coccolini; Marcello Mario D’Errico; Iris Agreiter; Giorgio Amadio Nespola; Francesco Barchiesi; Valeria Benigni; Raffaella Binazzi; Stefano Cappanera; Alessandro Chiodera; Valentina Cola; Daniela Corsi; Francesco Cortese; Massimo Crapis; Francesco Cristini; Alessandro D’Arpino; Belinda De Simone; Stefano Di Bella; Francesco Di Marzo; Abele Donati; Daniele Elisei; Massimo Fantoni; Anna Ferrari; Domitilla Foghetti; Daniela Francisci; Gianni Gattuso; Andrea Giacometti; Guido Cesare Gesuelli; Cristina Marmorale; Enrica Martini; Marcello Meledandri; Rita Murri; Daniela Padrini; Dalia Palmieri; Paola Pauri; Carla Rebagliati; Enrico Ricchizzi; Vittorio Sambri; Anna Maria Schimizzi; Walter Siquini; Loredana Scoccia; Giancarlo Scoppettuolo; Gabriele Sganga; Nadia Storti; Marcello Tavio; Giulio Toccafondi; Fabio Tumietto; Bruno Viaggi; Marco Vivarelli; Cristian Tranà; Melina Raso; Francesco Maria Labricciosa; Sameer Dhingra; Fausto Catena. 2020. "A proposal for a comprehensive approach to infections across the surgical pathway." World Journal of Emergency Surgery 15, no. 1: 1-26.

Journal article
Published: 15 February 2020 in European Journal of Clinical Microbiology & Infectious Diseases
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Clostridioides difficile infections are a significant threat to our healthcare system, and rapid and accurate diagnostics are crucial to implement the necessary infection prevention and control measurements. Nucleic acid amplification tests are such reliable diagnostic tools for the detection of toxigenic Clostridioides difficile strains directly from stool specimens. In this multicenter evaluation, we determined the performance of the revogene C. difficile assay. The analysis was conducted on prospective stool specimens collected from six different sites in Europe. The performance of the revogene C. difficile assay was compared to the different routine diagnostic methods and, for a subset of the specimens, against toxigenic culture. In total, 2621 valid stool specimens were tested, and the revogene C. difficile assay displayed a sensitivity/specificity of 97.1% [93.3-99.0] and 98.9% [98.5-99.3] for identification of Clostridioides difficile infection. Discrepancy analysis using additional methods improved this performance to 98.8% [95.8-99.9] and 99.6% [99.2-99.8], respectively. In comparison to toxigenic culture, the revogene C. difficile assay displayed a sensitivity/specificity of 93.0% [86.1-97.1] and 99.5% [98.7-99.9], respectively. These results indicate that the revogene C. difficile assay is a robust and reliable aid in the diagnosis of Clostridioides difficile infections.

ACS Style

Vittorio Sambri; Cécile Gateau; Silvia Zannoli; Giorgio Dirani; Jeanne Couturier; Ingrid Op Den Buijs; René Roymans; Emma Hallet; Mihaela Arnold; Anja Zumoberhaus; Stanislava Steiner; Jeroen Van De Bovenkamp; Martin Altwegg; Livia Berlinger; Frédéric Barbut; revogene C. Difficile study group; Johan Van Broeck. Diagnosing Clostridioides difficile infections with molecular diagnostics: multicenter evaluation of revogene C. difficile assay. European Journal of Clinical Microbiology & Infectious Diseases 2020, 39, 1169 -1175.

AMA Style

Vittorio Sambri, Cécile Gateau, Silvia Zannoli, Giorgio Dirani, Jeanne Couturier, Ingrid Op Den Buijs, René Roymans, Emma Hallet, Mihaela Arnold, Anja Zumoberhaus, Stanislava Steiner, Jeroen Van De Bovenkamp, Martin Altwegg, Livia Berlinger, Frédéric Barbut, revogene C. Difficile study group, Johan Van Broeck. Diagnosing Clostridioides difficile infections with molecular diagnostics: multicenter evaluation of revogene C. difficile assay. European Journal of Clinical Microbiology & Infectious Diseases. 2020; 39 (6):1169-1175.

Chicago/Turabian Style

Vittorio Sambri; Cécile Gateau; Silvia Zannoli; Giorgio Dirani; Jeanne Couturier; Ingrid Op Den Buijs; René Roymans; Emma Hallet; Mihaela Arnold; Anja Zumoberhaus; Stanislava Steiner; Jeroen Van De Bovenkamp; Martin Altwegg; Livia Berlinger; Frédéric Barbut; revogene C. Difficile study group; Johan Van Broeck. 2020. "Diagnosing Clostridioides difficile infections with molecular diagnostics: multicenter evaluation of revogene C. difficile assay." European Journal of Clinical Microbiology & Infectious Diseases 39, no. 6: 1169-1175.

Original article
Published: 15 February 2020 in European Journal of Clinical Microbiology & Infectious Diseases
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Clostridioides difficile infections are a significant threat to our healthcare system, and rapid and accurate diagnostics are crucial to implement the necessary infection prevention and control measurements. Nucleic acid amplification tests are such reliable diagnostic tools for the detection of toxigenic Clostridioides difficile strains directly from stool specimens. In this multicenter evaluation, we determined the performance of the revogene C. difficile assay. The analysis was conducted on prospective stool specimens collected from six different sites in Europe. The performance of the revogene C. difficile assay was compared to the different routine diagnostic methods and, for a subset of the specimens, against toxigenic culture. In total, 2621 valid stool specimens were tested, and the revogene C. difficile assay displayed a sensitivity/specificity of 97.1% [93.3–99.0] and 98.9% [98.5–99.3] for identification of Clostridioides difficile infection. Discrepancy analysis using additional methods improved this performance to 98.8% [95.8–99.9] and 99.6% [99.2–99.8], respectively. In comparison to toxigenic culture, the revogene C. difficile assay displayed a sensitivity/specificity of 93.0% [86.1–97.1] and 99.5% [98.7–99.9], respectively. These results indicate that the revogene C. difficile assay is a robust and reliable aid in the diagnosis of Clostridioides difficile infections.

ACS Style

Vittorio Sambri; revogene C. Difficile study group; Cécile Gateau; Silvia Zannoli; Giorgio Dirani; Jeanne Couturier; Ingrid Op Den Buijs; René Roymans; Emma Hallet; Mihaela Arnold; Anja Zumoberhaus; Stanislava Steiner; Jeroen van de Bovenkamp; Martin Altwegg; Livia Berlinger; Frédéric Barbut. Diagnosing Clostridioides difficile infections with molecular diagnostics: multicenter evaluation of revogene C. difficile assay. European Journal of Clinical Microbiology & Infectious Diseases 2020, 39, 1169 -1175.

AMA Style

Vittorio Sambri, revogene C. Difficile study group, Cécile Gateau, Silvia Zannoli, Giorgio Dirani, Jeanne Couturier, Ingrid Op Den Buijs, René Roymans, Emma Hallet, Mihaela Arnold, Anja Zumoberhaus, Stanislava Steiner, Jeroen van de Bovenkamp, Martin Altwegg, Livia Berlinger, Frédéric Barbut. Diagnosing Clostridioides difficile infections with molecular diagnostics: multicenter evaluation of revogene C. difficile assay. European Journal of Clinical Microbiology & Infectious Diseases. 2020; 39 (6):1169-1175.

Chicago/Turabian Style

Vittorio Sambri; revogene C. Difficile study group; Cécile Gateau; Silvia Zannoli; Giorgio Dirani; Jeanne Couturier; Ingrid Op Den Buijs; René Roymans; Emma Hallet; Mihaela Arnold; Anja Zumoberhaus; Stanislava Steiner; Jeroen van de Bovenkamp; Martin Altwegg; Livia Berlinger; Frédéric Barbut. 2020. "Diagnosing Clostridioides difficile infections with molecular diagnostics: multicenter evaluation of revogene C. difficile assay." European Journal of Clinical Microbiology & Infectious Diseases 39, no. 6: 1169-1175.

Journal article
Published: 16 December 2019 in Microorganisms
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The spread of carbapenem-resistant Enterobacteriaceae (CRE) has been enabled by the lack of control measures directed at carriers of multidrug-resistant organisms in healthcare settings. Screening patients for asymptomatic colonization on the one hand, and implementation of contact precautions on the other hand, reduces patient-to-patient transmission. Screening plates represents a relatively low-cost method for isolating CRE from rectal swabs; however, molecular assays have become widely available. This study compared the performance of four commercial molecular platforms in detecting clinically significant carbapenemase genes versus routine screening for CRE. A total of 1015 non-duplicated rectal swabs were cultured on a chromogenic carbapenem-resistant selective medium. All growing Enterobacteriaceae strains were tested for carbapenemase-related genes. The same specimens were processed using the following molecular assays: Allplex™ Entero-DR, Amplidiag® CarbaR + MCR, AusDiagnostics MT CRE EU, and EasyScreen™ ESBL/CPO. The prevalence of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae detected by swab culture was 2.2%, while organisms producing oxacillinase (OXA)-48 and metallo-β-lactamases were infrequent. The cost of CRE-related infection control precautions, which must be kept in place while waiting for screening results, are significant, so the molecular tests could become cost-competitive, especially when the turnaround time is decreased dramatically. Molecular assays represent a powerful diagnostic tool as they allow the rapid detection of the most clinically relevant carbapenemases.

ACS Style

Francesca Del Bianco; Manuela Morotti; Silvia Zannoli; Giorgio Dirani; Michela Fantini; Maria Federica Pedna; Patrizia Farabegoli; Vittorio Sambri. Comparison of Four Commercial Screening Assays for the Detection of blaKPC, blaNDM, blaIMP, blaVIM, and blaOXA48 in Rectal Secretion Collected by Swabs. Microorganisms 2019, 7, 704 .

AMA Style

Francesca Del Bianco, Manuela Morotti, Silvia Zannoli, Giorgio Dirani, Michela Fantini, Maria Federica Pedna, Patrizia Farabegoli, Vittorio Sambri. Comparison of Four Commercial Screening Assays for the Detection of blaKPC, blaNDM, blaIMP, blaVIM, and blaOXA48 in Rectal Secretion Collected by Swabs. Microorganisms. 2019; 7 (12):704.

Chicago/Turabian Style

Francesca Del Bianco; Manuela Morotti; Silvia Zannoli; Giorgio Dirani; Michela Fantini; Maria Federica Pedna; Patrizia Farabegoli; Vittorio Sambri. 2019. "Comparison of Four Commercial Screening Assays for the Detection of blaKPC, blaNDM, blaIMP, blaVIM, and blaOXA48 in Rectal Secretion Collected by Swabs." Microorganisms 7, no. 12: 704.

Review
Published: 26 June 2019 in Microorganisms
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West Nile virus (WNV) and Usutu virus (USUV) are neurotropic mosquito-borne flaviviruses that may infect humans. Although WNV is much more widespread and plays a much larger role in human health, the two viruses are characterized by similar envelope antigens, clinical manifestations, and present overlapping in terms of geographic range of transmission, host, and vector species. This review highlights some of the most relevant aspects of WNV and USUV human infections in Europe, and the possible implications of their co-circulation.

ACS Style

Silvia Zannoli; Vittorio Sambri. West Nile Virus and Usutu Virus Co-Circulation in Europe: Epidemiology and Implications. Microorganisms 2019, 7, 184 .

AMA Style

Silvia Zannoli, Vittorio Sambri. West Nile Virus and Usutu Virus Co-Circulation in Europe: Epidemiology and Implications. Microorganisms. 2019; 7 (7):184.

Chicago/Turabian Style

Silvia Zannoli; Vittorio Sambri. 2019. "West Nile Virus and Usutu Virus Co-Circulation in Europe: Epidemiology and Implications." Microorganisms 7, no. 7: 184.

Journal article
Published: 30 September 2018 in International Journal of Sports and Exercise Medicine
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ACS Style

Giovanni Grazzi; Valentina Totti; Jonathan Myers; Giovanni Mosconi; Camilla Gambaretto; Vittorio Sambri; Manuela Trerotola; Alessandro Nanni Costa; Gianluigi Sella. High Level Cycling Performance 10 Years after Cardiac Transplantation. International Journal of Sports and Exercise Medicine 2018, 4, 1 .

AMA Style

Giovanni Grazzi, Valentina Totti, Jonathan Myers, Giovanni Mosconi, Camilla Gambaretto, Vittorio Sambri, Manuela Trerotola, Alessandro Nanni Costa, Gianluigi Sella. High Level Cycling Performance 10 Years after Cardiac Transplantation. International Journal of Sports and Exercise Medicine. 2018; 4 (3):1.

Chicago/Turabian Style

Giovanni Grazzi; Valentina Totti; Jonathan Myers; Giovanni Mosconi; Camilla Gambaretto; Vittorio Sambri; Manuela Trerotola; Alessandro Nanni Costa; Gianluigi Sella. 2018. "High Level Cycling Performance 10 Years after Cardiac Transplantation." International Journal of Sports and Exercise Medicine 4, no. 3: 1.

Book chapter
Published: 01 January 2018 in Chikungunya and Zika Viruses
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ACS Style

Claudia Chiesa; Agnese Denicolò; Ibrahima Dia; Cheikh T. Diagne; Diawo Diallo; Mawlouth Diallo; Alioune Gaye; Scott B. Halstead; Stephen Higgs; Susan Hills; Yan-Jang S. Huang; Stefan W. Metz; Thomas P. Monath; Manuela Morotti; Thomas E. Morrison; Anna Pierro; Gorben P. Pijlman; Ann M. Powers; Jamal I-Ching Sam; Vittorio Sambri; Martina Tassinari; Dana L. VanLandingham; Pedro F.C. Vasconcelos; Silvia Zannoli. Contributors. Chikungunya and Zika Viruses 2018, 1 .

AMA Style

Claudia Chiesa, Agnese Denicolò, Ibrahima Dia, Cheikh T. Diagne, Diawo Diallo, Mawlouth Diallo, Alioune Gaye, Scott B. Halstead, Stephen Higgs, Susan Hills, Yan-Jang S. Huang, Stefan W. Metz, Thomas P. Monath, Manuela Morotti, Thomas E. Morrison, Anna Pierro, Gorben P. Pijlman, Ann M. Powers, Jamal I-Ching Sam, Vittorio Sambri, Martina Tassinari, Dana L. VanLandingham, Pedro F.C. Vasconcelos, Silvia Zannoli. Contributors. Chikungunya and Zika Viruses. 2018; ():1.

Chicago/Turabian Style

Claudia Chiesa; Agnese Denicolò; Ibrahima Dia; Cheikh T. Diagne; Diawo Diallo; Mawlouth Diallo; Alioune Gaye; Scott B. Halstead; Stephen Higgs; Susan Hills; Yan-Jang S. Huang; Stefan W. Metz; Thomas P. Monath; Manuela Morotti; Thomas E. Morrison; Anna Pierro; Gorben P. Pijlman; Ann M. Powers; Jamal I-Ching Sam; Vittorio Sambri; Martina Tassinari; Dana L. VanLandingham; Pedro F.C. Vasconcelos; Silvia Zannoli. 2018. "Contributors." Chikungunya and Zika Viruses , no. : 1.

Book chapter
Published: 01 January 2018 in Chikungunya and Zika Viruses
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ACS Style

Silvia Zannoli; Manuela Morotti; Agnese Denicolò; Martina Tassinari; Claudia Chiesa; Anna Pierro; Vittorio Sambri. Chikungunya Virus and Zika Virus in Europe. Chikungunya and Zika Viruses 2018, 193 -214.

AMA Style

Silvia Zannoli, Manuela Morotti, Agnese Denicolò, Martina Tassinari, Claudia Chiesa, Anna Pierro, Vittorio Sambri. Chikungunya Virus and Zika Virus in Europe. Chikungunya and Zika Viruses. 2018; ():193-214.

Chicago/Turabian Style

Silvia Zannoli; Manuela Morotti; Agnese Denicolò; Martina Tassinari; Claudia Chiesa; Anna Pierro; Vittorio Sambri. 2018. "Chikungunya Virus and Zika Virus in Europe." Chikungunya and Zika Viruses , no. : 193-214.

Book chapter
Published: 01 January 2018 in Chikungunya and Zika Viruses
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ACS Style

Silvia Zannoli; Manuela Morotti; Agnese Denicolò; Martina Tassinari; Claudia Chiesa; Anna Pierro; Vittorio Sambri. Diagnostics and Laboratory Techniques. Chikungunya and Zika Viruses 2018, 293 -315.

AMA Style

Silvia Zannoli, Manuela Morotti, Agnese Denicolò, Martina Tassinari, Claudia Chiesa, Anna Pierro, Vittorio Sambri. Diagnostics and Laboratory Techniques. Chikungunya and Zika Viruses. 2018; ():293-315.

Chicago/Turabian Style

Silvia Zannoli; Manuela Morotti; Agnese Denicolò; Martina Tassinari; Claudia Chiesa; Anna Pierro; Vittorio Sambri. 2018. "Diagnostics and Laboratory Techniques." Chikungunya and Zika Viruses , no. : 293-315.

Journal article
Published: 30 December 2017 in Microbiologia Medica
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Background and aims. Bacterial vaginosis (BV) is one the more frequently identified genital syndrome among childbearing aged women. The basic condition that generates this condition is a modification in the vaginal microbiota. The aim of this paper is to briefly review the current status of the art of BV and to report the results of a pilot study performed with an innovative PCR based technique. Materials and Methods. 36 samples of vaginal fluid routinely submitted for the diagnosis of BV to the Unit of Microbiology – GRHL were comparatively evaluated by standard techniques and with the HP-Vaginiti e Vaginosi NLM kit that simultaneously detects in a quantitative way specific DNA from Candida (albicans, glabrata; krusei, tropicalis), Gardnerella vaginalis, Lactobacillus spp. and Atopobium vaginae. Results and conclusions. Candida spp. has been identified in 8 samples with culture and in 15 with the molecular test. 29 G. vaginalis were found by PCR whereas only in 7 samples a specific prescription for this microbe was present (of which 4 positive). A. vaginae has been identified in 20 samples by the molecular approach and Lactobacillus spp. was identified in 19 samples (by culture) and in 32 by PCR. The overall diagnosis of BV was made in 9 patients by standard techniques and in 7 by applying the molecular approach. (Cohen’s kappa test: 0,84). The findings of this study clearly demonstrate that the joint use of the routine culture- based techniques with the multiplex PCR methods amplifies by far the sensitivity of the overall diagnostic workflow of BV.

ACS Style

Giorgio Dirani; Silvia Zannoli; Maria Federica Pedna; Francesco Congestri; Patrizia Farabegoli; Barbara Dalmo; Vittorio Sambri. Bacterial vaginosis: epidemiologic, clinical and diagnostic updates. Microbiologia Medica 2017, 32, 1 .

AMA Style

Giorgio Dirani, Silvia Zannoli, Maria Federica Pedna, Francesco Congestri, Patrizia Farabegoli, Barbara Dalmo, Vittorio Sambri. Bacterial vaginosis: epidemiologic, clinical and diagnostic updates. Microbiologia Medica. 2017; 32 (4):1.

Chicago/Turabian Style

Giorgio Dirani; Silvia Zannoli; Maria Federica Pedna; Francesco Congestri; Patrizia Farabegoli; Barbara Dalmo; Vittorio Sambri. 2017. "Bacterial vaginosis: epidemiologic, clinical and diagnostic updates." Microbiologia Medica 32, no. 4: 1.

Journal article
Published: 30 December 2017 in Microbiologia Medica
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Molecular techniques for the detection of the antimicrobial sensitivity: friend or foe?

ACS Style

Vittorio Sambri. Molecular techniques for the detection of the antimicrobial sensitivity: friend or foe? Microbiologia Medica 2017, 32, 1 .

AMA Style

Vittorio Sambri. Molecular techniques for the detection of the antimicrobial sensitivity: friend or foe? Microbiologia Medica. 2017; 32 (4):1.

Chicago/Turabian Style

Vittorio Sambri. 2017. "Molecular techniques for the detection of the antimicrobial sensitivity: friend or foe?" Microbiologia Medica 32, no. 4: 1.