Precise classification of seeds is important for agriculture. Due to the slight physical and chemical difference between different types of wheat and high correlation between bands of images, it is easy to fall into the local optimum when selecting the characteristic band of using the spectral average only. In this paper, in order to solve this problem, a new variable fusion strategy was proposed based on successive projection algorithm and the variable importance in projection algorithm to obtain a comprehensive and representative variable feature for higher classification accuracy, within spectral mean and spectral standard deviation, so the 25 feature bands obtained are classified by support vector machine, and the classification accuracy rate reached 83.3%. It indicates that the new fusion strategy can mine the effective features of hyperspectral data better to improve the accuracy of the model and it can provide a theoretical basis for the hyperspectral classification of tiny kernels.

Recent findings suggest that cold acclimation can enhance cold resistance in wheat. Dongnongdongmai 1 (DM1) is a winter wheat variety that can overwinter at −30 °C; however, its cold acclimation mechanism is yet to be fully elucidated. Here, we elucidated the potential mechanisms of cold acclimation in DM1 and the China Spring (CS) variety, especially the role of the antioxidant system, using transcriptome and physiological analyses. Cold stress increased H₂O₂ and O₂⁻ production in both varieties; however, CS had higher contents of H₂O₂ and O₂⁻ than DM1. Moreover, cold significantly increased ROS-scavenging activities in DM1, especially at 30 days after exposure. Gene ontology (GO) analysis showed that differentially expressed peroxidase (POD) genes were enriched in antioxidant activity, with most POD genes being significantly upregulated in DM1 under cold acclimation. Additionally, cold acclimation increased the expression of cold acclimation protein (CAP), late embryogenesis abundant protein (LEA), and cold-responsive genes in both varieties, with higher expression levels in DM1. Overall, the results showed that DM1 exhibited a higher cold tolerance than CS during cold acclimation by increasing the expression of POD genes, LEA, CAP, and cold-responsive proteins, improving the understanding of the mechanism of cold resistance in DM1.