Cancer survivors undergo meticulous examinations, including regular magnetic resonance imaging (MRI) scans, to monitor the risk of disease recurrence. The use of magnetic iron nanoparticles (MNPs) enhances MRI accuracy. However, post-injection, MNPs exhibit a notable affinity for binding with proteins and biomolecules, forming a dynamic protein coating called a protein corona (CORONA). While there are reports of its elimination in the liver and kidney metabolism system, patients undergoing this method have shown symptoms of liver problems and related enzyme alterations. This study aims to discern whether the impact of MNPs on liver enzymes significantly contributes to liver damage. The investigation focuses on the effects of magnetic nanoparticles (MNPs) on selected enzymes, including alanine aminotransferase (ALT), aspartate transaminase (AST), α-amylase, and lipase. Employing 104 experiments over a central composite design (CCD), the study evaluates the effects of agents on MNP and enzyme structure, stability, and properties: enzyme assay, electron microscopy, and circular dichroism of secondary structure after interaction with MNPs. The study’s findings unveil the intricate relationship between MNPs and liver enzymes, providing valuable insights for clinical practices and refining the safety profile of MRI. This comprehensive exploration contributes to our understanding of potential implications and aids in optimizing the use of MNPs in medical imaging for cancer survivors.

IJPAB - 2015 Volume 3, issue 6

Background: Diabetes is a contemporary ailment characterized mainly by elevated blood glucose levels. Diabetic foot ulcers (DFUs) significantly impact the patient’s quality of life and pose challenges for the healthcare system. Microalgae have emerged as effective agents for controlling inflammation owing to their elevated levels of active chemicals. Objectives: In the present study, we isolated and identified the Haematococcus pluvialis species of freshwater algae and investigated its effect on inhibiting the growth of diabetic wound bacteria. Methods: The ethanolic extract of this algae was investigated on antibiotic-resistant Staphylococcus aureus isolated from antibiotic-resistant wounds of diabetic patients. For this statistical study, a central composite design was used, considering factors such as gender, age, level of non-healing of the wound, and the effectiveness of the extract in inhibiting bacterial growth as the response. Results: The results showed that compared to other extracts, the ethanolic extract effectively inhibits bacterial growth up to 70% and 30%, respectively, of levofloxacin and ceftazidime antibiotics. The study of this extract with liquid chromatography-mass spectrometry led to the identification of the astaxanthin compound as the main component of the extract. Conclusions: This study has substantial prospects for future therapeutic uses and instills hope for enhanced therapies for DFUs.