Background: The purpose of this study was to determine the anatomical variations of the root canal system of mandibular second molars using cone-beam computed tomography (CBCT). Methods: 190 mandibular second molars cone-beam computed tomography images were reviewed. The evaluation was performed by a radiologist with endodontic experience and two endodontists trained with CBCT technology. Tooth position, number of root and root canals, C-shaped root canal system configuration, presence of extra root (radix), and radicular grooves were assessed. Data was statistically analyzed using The Chi-square test (α = 0,05) to determine any significant difference between gender and the total number of root and root canals, and any significant difference between gender and root canal anatomical variation. Results: Overall, 85.5% showed two separated roots, 12.1% a single root, 2.6% three roots or radix. 87.7% showed three root canals, 12.1% two root canals, 2.6% four root canals, and 1.6% a single root canal. 10% showed a single foramen, 75.3% two foramina, 13.6% three foramina and 1% showed four foramina.19.5% showed C-shaped anatomical variation, 51.4% in male patients, 48.6% in female patients. According to Fan classification: C1 13.6% in cervical third, C2 10% in the middle third, C3 17.3% in middle third, 15.5% in apical third, and C4 12.7% in the apical third. Root canals number in these samples were 5.4% a single canal, 21.6% two canals, 70.3% three canals, and 2.7% four canals. The root showed 46% with one foramen, 46% two foramina, and 8% three foramina. Radicular grooves 83.3% were found in the lingual area and 16.2% towards the buccal area. Conclusions: The most prevalent anatomic presentation of the evaluated sample was a mandibular second molars with two roots, three root canals, and two apical foramina. Their variation was C-shaped root canals and Radix Paramolaris.

Background: The aim of this study was to measure the dental pulp inflammatory response through neuropeptides (SP and CGRP) as a response to occlusal trauma, orthodontic movements and a combination of both, as well as the angiogenic defense mechanism through VEGF expression, which could be the initial step to mineralized tissue formation. Methods: Forty human dental pulp samples were collected from healthy first premolars with extraction indicated due to orthodontic reasons from a sample of 20 patients. Patients were divided into four groups with 10 premolars each (1 mandibular and 1 maxillary premolar from each patient): healthy pulp control group, occlusal trauma group, moderate orthodontic forces group; and occlusal trauma plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The Kruskal–Wallis test was performed to assess significant differences among groups and Mann–Whitney’s U post hoc pairwise comparisons were also performed. Results: The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal trauma and the control groups, with statistically significant differences between all groups for each of the 3 peptides analyzed (Kruskal–Wallis p < 0.001). All possible pairwise post-hoc comparisons were also significant for each peptide analyzed (Mann–Whitney’s U p < 0.001). Conclusion: SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal trauma combined with moderate orthodontic forces, as compared with these two stimuli applied independently. Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive Response. Trial registration number: NCT03804034. Date of registration: 01/15/2019 Retrospectively registered. URL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1.

Background: Mandibular premolars are complicated teeth to endodontically treat due to the anatomical variations that can present. The purpose of this study was to determine the presence of C-shaped configurations in mandibular premolars by cone-beam computed tomography (CBCT). Methods: 380 mandibular first premolars and 308 mandibular second premolars cone-beam computed tomographic images were obtained from 292 patients (175 female and 117 male). Tooth position, number of roots, root canals, C-shaped root canal system configuration, level of canal bifurcation and radicular grooves were evaluated by two endodontists trained in CBCT evaluation; previously calibrated between them, and a radiologist with endodontic experience. Data were statistically compared by The Chi-square test (α = 0.05) to examine any significant difference between gender and C-shaped root canal system and any significant difference between C-shaped configuration according to Fan criteria and gender. Results: Overall 100% (n = 688) teeth examined, 19.2% (n = 132) had a C-shaped root canals system. 9.16% (n = 63) in male patients and 10.03% (n = 69) in female patients. The prevalence of C-shaped root canal system in mandibular first premolar was 83.33 and 16.66% in mandibular second premolars. According to Fan classification, the mandibular first premolars showed 3.63% as C1, 9.09% C2, 59.09% C3, 21.81% C4a, 1.8% C4b. Mandibular second premolars showed 13.63% as C1, 18.18% C2, 13.63% C3, 27.27% C4a, 9.09% C4b. Male patients showed 4.54% as C1, 3.78% C2, 22.72% C3, 11.36% C4a, 0.75% C4b, and 4.54% out of classification. Female patients showed 0.75% as C1, 6.81% C2, 30.03% C3, 9.84 C4a, 2.27% C4b, and 2.27% out of classification. The 53.36% canal bifurcation in mandibular first premolars and 50.09% in mandibular second premolars were in the middle third. No statistical differences were found between C-shaped root canal system and gender or C-shaped configuration according to Fan criteria and gender. P was < 0.05. Conclusions: Within the limitation of this study, the presence of C-shaped anatomical system is relatively low in mandibular second premolars than in mandibular first premolars.