Ferulic acid (FA) has antioxidative and anti-inflammatory effects, and is a promising drug to treat sepsis.To study the therapeutic effect of FA in sepsis-induced acute lung injury (ALI) and its un...

Asthma is a heterogeneous disease, and abnormal activation of T cells is the driving link of asthmas pathophysiological changes. Dual-positive Th2Th17 cells, as newly discovered T-helper cells, have the functions of Th2 and Th17 cells and can coproduce Th2 and Th17 cytokines. Previous studies have shown that dual-positive Th2Th17 cells increase the chances of asthma and correlate with asthma severity. However, the exact role of dual-positive Th2Th17 cells in asthma is not known. Since there is no mature differentiation method for dual-positive Th2Th17 cells, the present study aimed to clarify the strict differentiation conditions and reveal how dual-positive Th2Th17 cells regulate asthma phenotypes. In this study, we confirmed that IL-1β, IL-6, anti-IFN-γ, and IL-21 promoted biphenotypic cell differentiation. Moreover, more proportion of dual-positive Th2Th17 cells can be obtained by conditioned differentiation of mouse CD4⁺ T cells after classical allergic asthma modeling. Before asthma modeling, adoptive dual-positive Th2Th17 cells promoted T cells to differentiate into the same biphenotype cells and exacerbated the severity of asthma. Together, our results clarify the differentiation conditions of dual-positive Th2Th17 cells and further confirm that it stimulates asthma T cells to differentiate into the same biphenotype cells, leading to exacerbation of asthma.