Chronic thromboembolic pulmonary hypertension (CTEPH) develops in 1.5–2.0% of patients experiencing pulmonary embolism (PE) and is characterized by stable pulmonary artery obstruction, heart failure, and poor prognosis. Little is known about involvement of autonomic nervous system (ANS) in the mechanisms of CTEPH. This study was aimed at evaluation of the effect of vagal and sympathetic denervation, as well as stimulation of the parasympathetic nervous system, on the outcomes of CTEPH in rats. CTEPH was induced by multiple intravenous injections of alginate microspheres. Sympathetic and vagal denervation was performed using unilateral surgical ablation of the stellate ganglion and vagotomy, respectively. Stimulation of the parasympathetic nervous system was carried out by administering pyridostigmine. The effect of neuromodulatory effects was assessed in terms of hemodynamics, histology, and gene expression. The results demonstrated the key role of ANS in the development of CTEPH. Sympathetic denervation as well as parasympathetic stimulation resulted in attenuated pulmonary vascular remodeling. These salutary changes were associated with altered MMP2 and TIMP1 expression in the lung and decreased FGFb level in the blood. Unilateral vagotomy had no effect on physiological and morphological outcomes of the study. The data obtained contribute to the identification of new therapeutic targets for CTEPH treatment.

The in silico modelling of peptides complementary to lactoferrin was carried out using the Protein 3D software package and replication of the natural bonding site between pneumococcal surface protein (PSP) and lactoferrin (LF). The modeling was based on analysis of the conjugated ion–hydrogen bond systems between these proteins (CIHBS). The oligopeptide EEVAPQAQAKIAELENQVHRLE was proposed via computer modelling and synthesized using the solid phase synthesis technique, purified, and analyzed with MS and HPLC methods to confirm >95% purity. The peptide was then studied by capillary electrophoresis (CE). The CE experiments demonstrated the split of peptide zone in the presence of LF, due to complex formation and subsequent mobility change of the system peptide-protein. The reference experiments with homomyeloperoxidase and myoglobin did not show binding with LETI-11.

Objective: to develop algorithm of multiple comparisons data of prospective non-randomized clinical trial AMIRI CABG (ClinicalTrials.gov Identifier: NCT03050489) using SAS Enterprise Guide 6.1. Materials and methods. Data collection was performed according prospective non-randomized clinical trial AMIRI CABG in Pavlov First St. Petersburg State Medical University, Saint Petersburg, Russia between 20162019 years with 336 patients. There is database with clinical, laboratory and instrumental data. Multiple comparisons test was performed with SAS Enterprise Guide 6.1. Results. There was developed algorithm of multiple comparisons data of prospective non-randomized clinical trial AMIRI CABG for independent group. This algorithm could be useful for physicians and researchers for data analysis. Conclusion. Presented algorithm of data analysis could make easier and improve efficient data analysis. SAS Enterprise Guide 6.1 allows fast and accurate process data

Background. Prostate cancer is one of the most common malignant neoplasms in the male population worldwide with high morbidity and mortality rates.Aim. To study the main medial statistical indicators of prostate cancer in Saint Petersburg and other regions of the Russian Federation.Materials and methods. The epidemiological indicators of prostate cancer for 2012-2021 in the Russian Federation, individual federal districts and Saint Petersburg were analyzed.Results. The prevalence of malignant neoplasms of the prostate has increased in recent years. At the same time, the number of cases of early-stage disease diagnosis has increased, one-year mortality has decreased, and 5-year survival has increased, which indicates proper quality of oncological care for the population. In some federal districts, unstable or negative dynamics of indicators compared to the average Russian data are observed, which requires further analysis.Conclusion. Evaluation of statistical measures of morbidity and mortality from malignant neoplasms of the prostate gland can be used to improve diagnostic algorithms and therapeutic tactics for this pathology.

Introduction. Chronic thromboembolic pulmonary hypertension (CTEPH) is one of the most severe complications of pulmonary embolism (PE), characterized by poor prognosis and insuffcient effectiveness of standard treatment approaches. A small number of representative models of CTEPH make it diffcult to conduct preclinical studies of promising pharmacological substances.Objective – development and validation of the experimental model of CTEPH in rats by embolization of the distal branches of the pulmonary artery with biodegradable microspheres.Material and methods. Male Wistar rats were used for the experiments. Biodegradable microspheres (MS) based on sodium alginate and autologous blood clots (AT) were used as embolizing particles. The animals were divided into groups: control: saline solution was injected 4 times with an interval of 8 days into the tail vein; AT: according to the above protocol, 50 μL of AT was injected; MS was administered intravenously in a volume of 50 μl of MS according to two protocols: MS4: 4 times with an interval of 8 days; MS8: 8 times with an interval of 4 days. After 2 and 6 weeks after the last injection, a histological examination of the lungs was performed; after 6 weeks: echocardiographic study (TTE), right ventricular catheterization (RV) with measurement of right ventricular systolic pressure (RVSP), treadmill test, assessment of serum endothelin­1 levels by the immunoassay method.Results. During the experiments, the survival rate in the MS8 group was 50 %. In the other groups, there were no animal losses. According to the treadmill test 6 weeks after the modeling of PE, exercise tolerance was signifcantly reduced in the MC4 and MC8 groups compared with the control group. TTE data indicate a signifcant increase in the diameter of the pulmonary trunk and the right ventricular outflow tract in the MC8 compared with the control and AT. There were signifcant increase in RVSP and the level of endothelin­1 compared with the control only in the MS8. After 6 weeks, the index of hypertrophy of vessel wall of the pulmonary artery in the MC4 and MC8 was signifcantly higher compared with the control and AT groups.Conclusion. Based on the use of MS, administered under the MS 8 protocol, a new representative model of CTEPH has been created, which can be used to test promising pharmacological substances.

Objective. To develop algorithm of data analysis of prospective non-randomized clinical trial AMIRICABG (ClinicalTrials.gov Identifier: NCT03050489) using SAS Enterprise Guide 6.1. Materials and methods. Data collection was performed according prospective non-randomized clinical trial AMIRICABG in Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia between 20162019 years with 336 patients. There is database with clinical, laboratory and instrumental data. Statistical analysis was performed with SAS Enterprise Guide 6.1. Results. There was developed algorithm of data analysis of prospective non-randomized clinical trial AMIRICABG. This algorithm could be useful for physicians and researchers for data analysis. Conclusion. Presented algorithm of data analysis could make easier and improve efficient data analysis. SAS Enterprise Guide 6.1 allows fast and accurate process big data.