Salted duck egg white (SDEW), as the main by-product in the production process of salted egg yolk, has not been effectively used as a food resource because of its high salt concentration. This study creatively used isoelectric point precipitation, ultrafiltration, and cation exchange to separate and purify lysozyme from SDEW and preliminarily explored the enzymatic properties of lysozyme. The results showed that the relative molecular weight of lysozyme was about 14 KDa, and the specific activity of lysozyme reached 18,300 U/mg. Lysozyme had good stability in the temperature range of 30 °C to 60 °C and pH of 4 to 7. Metal ions, Fe2+, Cu2+, and Zn2+, strongly inhibited lysozyme activity. Different surfactants showed certain inhibition effects on lysozyme from SDEW, among which glycerin had the strongest inhibitory effect. This study aimed to provide a theoretical reference for industrial purification and production of lysozyme from SDEW.
Utilizing gamma rays as an initiating agent, a simultaneous irradiation method was applied to graft acrylic acid and acrylamide onto corn straw that had been decrystallized using a NaOH/urea solution at a reduced temperature, aiming to fabricate superabsorbent polymer gel (SAPG) capable of absorbing significantly more water. The structural attributes of the corn straw, the decrystallized corn straw, and the SAPG were analyzed via Fourier transform infrared spectroscopy (FTIR), X-ray crystal powder diffraction (XRD), thermogravimetric analysis (TG), and scanning electron microscopy (SEM). To enhance the SAPG’s performance, optimization of various parameters was carried out, such as irradiation dose, dose rate, the ratio of monomer to corn straw, the proportion of acrylic acid (AA) to acrylamide (Am), and the degree of neutralization. The resulting SAPG exhibited distilled water absorption of 1033 g/g and 90 g/g in 0.9 wt.% NaCl solution, with a radiation dose of 5 kGy, a dose rate of 1.5 kGy/h, AA-to-AM mass ratio of 1.2, monomer-to-CS mass ratio of 7, and 90% AA neutralization.
Salted duck egg is one of the most popular products, and China is one of the major countries consuming salted duck egg products. However, due to the high salt content of salted egg white and low physical and chemical properties such as gel, many factories generally only use salted egg yolk and discard salted duck egg white (SDEW) as a waste liquid when processing. This is not only a waste of resources, but also a pollution to the environment. In this paper, protein powder was prepared from salted egg white. Then xanthan gum (XG) was added to make it co-gel with ovalbumin to achieve the purpose of preparing high gelatinous salted egg white protein powder. The results showed that the optimum conditions of SDEW-XG composite gel were as follows: the xanthan gum content was 0.08% (w/w), the reaction pH was 6.5, and the heating temperature was 100°C. Under these conditions, the gel strength reaches the maximum value. Meanwhile, compared with the protein powder without xanthan gum, the addition of xanthan gum significantly affected the secondary structure of the protein powder of SDEW and improved the water holding capacity of the gel. In conclusion, the addition of xanthan gum can significantly improve the gel quality of SDEW protein powder, which provides a theoretical basis for the quality improvement of salted egg white.
1 January 2022 in Science and Engineering of Composite Materials
A novel superabsorbent copolymer (SAP) was developed by grafting acrylic acid onto the pretreated soybean straw (PSBS) using gamma-ray irradiation. The structure of soybean straw, treated soybean straw, and SAP were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TG), and scanning electron microscopy (SEM). The effect of irradiation dose, the dosage of cross-link agent, and the monomer ratio of the graft copolymer on the water absorbency of the SAP was investigated. The highest water absorbency of 1,489 g/g in distilled water and 56 g/g in 0.9 wt% NaCl solution was obtained, when the ratio of acrylic acid (AA) to PSBS was 6:1. This irradiation technique is superior to traditional methods with advantages such as less heavy pollution, low-energy consumption, and high-water retention performance. The outcome is deemed to open up new pathways to synthesize environmentally safe superabsorbents with applications in the food, pharmaceutical, and medical industries.