Tight oil and gas resources are widely distributed and play an important role in the petroleum industry. Due to its nanoscale pore-throat characteristics, the capillary effect is remarkable, and spontaneous imbibition is very beneficial to the development of low-permeability reservoirs. In this study, the imbibition experiments of 2D nano blackcard, nanoemulsion, and water were carried out, respectively. The pore-throat fluid distribution characteristics before and after core imbibition were analyzed with nuclear magnetic resonance technology, and the enhanced oil recovery effects of 2D nano blackcard nanoemulsion, and water were comprehensively evaluated. The results show that the final recovery factors of cores soaked in 2D nano blackcard (0.005 ωt%) and nanoemulsion (0.02 ωt%) or imbibed in water are 32.29%, 26.05%, and 7.19%, respectively. It can be found that 2D nano blackcard is the fluid with the best imbibition effect. In this work, a new type of 2D nano blackcard was proposed and identified as a functional imbibition fluid for enhanced oil recovery in tight reservoirs, providing a practical reference for the effective development of tight, low-permeability oil and gas reservoirs.