Ultraviolet (UV) exposure has been linked to skin damage and carcinogenesis, but recently UVB has been proposed as a therapeutic approach for cancer. Herein, we investigated the cellular and molecular effects of UVB in immortal and tumorigenic HPV positive and negative cells. Cells were irradiated with 220.5 to 1102.5 J/m² of UVB and cell proliferation was evaluated by crystal violet, while cell cycle arrest and apoptosis analysis were performed through flow cytometry. UVB effect on cells was recorded at 661.5 J/m² and it was exacerbated at 1102.5 J/m². All cell lines were affected by proliferation inhibition, cell cycle ablation and apoptosis induction, with different degrees depending on tumorigenesis level or HPV type. Analysis of the well-known UV-responsive p53, E2F1 and microtubules system proteins was performed in SiHa cells in response to UVB through Western-blotting assays. E2F1 and the Microtubule-associated protein 2 (MAP2) expression decrease correlated with cellular processes alteration while p53 and Microtubule-associated Protein 1S (MAP1S) expression switch was observed since 882 J/m², suggesting they were required under more severe cellular damage. However, expression transition of α-Tubulin3C and β-Tubulin was abruptly noticed until 1102.5 J/m² and particularly, γ-Tubulin protein expression remained without alteration. This study provides insights into the effect of UVB in cervical cancer cell lines.

Crataegus oxyacantha has been mainly used in traditional medicine for the treatment of cardiovascular diseases. However, its safety profile has not been fully established, since only the genotoxic effects of C. oxyacantha fruit have been described. Therefore, the objective of this work was evaluating the cytotoxicity and genotoxicity of the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha by means of the micronucleus test in a murine model. Doses of 2000, 1000, and 500 mg/kg of both extracts were administered orally for 5 days in mice of the Balb-C strain. Peripheral blood smears were performed at 0, 24, 48, 72, and 96 h after each administration. The number of polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MNPCEs), and micronucleated erythrocytes (MNEs) was determined at the different sampling times. Our results showed that the leaf and bark of C. oxyacantha increase the number of MNEs at the 2000 mg/kg dose, and only the aqueous leaf extract decreases the number of PCEs at the same dose. Therefore, the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha showed genotoxic effects, and only the aqueous leaf extract exhibited cytotoxic effects.

Introducción: Este estudio nace por el interés de analizar si la educación nutricional que reciben las y los adolescentes en su formación dentro del nivel medio superior, les brinda los criterios para elegir información alimentaria a través de las redes sociales de fuentes fidedignas, es decir, de profesionistas relacionados a la Nutrición que basen su contenido y publicaciones de manera científica y objetiva. Las redes sociales cuentan con gran popularidad, y, algunas personas lo aprovechan para promocionar productos o divulgar información falsa, es por ello que se debe concientizar sobre el buen uso de estas plataformas digitales. Metodología: Se realizó un estudi de caso a 35 estudiantes de la preparatoria "Villanueva" ubicada en el municipio del mimo nombre, perteneciente al estado de Zacatecas, México, de la "Formación para el trabajo de higiene y salud comunitaria", la cual, contempla en su plan de estudios un módulo sobre educación nutricional. Se aplicaron dos encuestas semiestructuradas durante el ciclo escolar 2022-2023, de las cuales se recabaron datos relacionados al uso de las redes sociales, tiempo que dedican a navegar en ellas, cuales son las que mayormente utilizan, así como el tipo de información alimentaria que visualizan en estos medios digitales. Resultados: La edad aproximada de las y los encuestados osciló entre los 17 años de edad, de los cuales, 20 fueron mujeres, 9 hombres y 6 no especificaron su sexo, así mismo del total de la muestra el 5.1% presentaban obesidad, el 10.2% sobrepeso y el 64.1% tenían normo peso. El 100% de las y los encuestados afirmó tener al menos una cuenta activa en redes sociales, donde refirieron seguir perfiles que hablen sobre nutrición, considerando que la información es más atractiva a través de estos medios digitales. Discusión: Se demostró que las y los adolescentes encuestados no cuentan con los criterios correctos para discernir entre la información nutricional que visualizan en las redes sociales, ya que hubo mayores coincidencias en seguir las cuentas, por ejemplo: Leana Deeb, Carlos Belcast y Andoni, influencers que no cuentan con preparación académica relacionada a la nutrición. Conclusiones: Las y los adolescentes refirieron hacer uso frecuente de las redes sociales y las consideran como un elemento importante en su vida para la comunicación y obtención de información, sin embargo, se encontró confusión al momento de reconocer cuáles personas cuentan con preparación académica en temas de Nutrición.

The anticarcinogenic potential of a series of 1,5-disubstituted tetrazole-1,2,3-triazole hybrids (T-THs) was evaluated in the breast cancer (BC)-derived cell lines MCF-7 (ER+, PR+, and HER2−), CAMA-1 (ER+, PR+/−, and HER2−), SKBR-3 (ER+, PR+, and HER2+), and HCC1954 (ER+, PR+, and HER2+). The T-THs 7f, 7l, and 7g inhibited the proliferation of MCF-7 and CAMA-1, HCC1954, and SKBR-3 cells, respectively. The compounds with stronger effect in terms of migration and invasion inhibition were 7o, 7b, 7n, and 7k for the CAMA-1, MCF-7, HCC1954, and SKBR-3 cells respectively. Interestingly, these T-THs were the compounds with a fluorine present in their structures. To discover a possible target protein, a molecular docking analysis was performed for p53, p38, p58, and JNK1. The T-THs presented a higher affinity for p53, followed by JNK1, p58, and lastly p38. The best-predicted affinity for p53 showed interactions between the T-THs and both the DNA fragment and the protein. These results provide an opportunity for these compounds to be studied as potential drug candidates for breast cancer treatment.

The use of plants has grown constantly worldwide, being a rich source of compounds that serve as established treatments for various diseases and conditions. This paper discusses the elemental composition and the level of environmental risk of heavy metals of selected medicinal plants. The extracts are investigated by energy dispersive X-ray fluorescence, a non-destructive, fast, multi-element, highly accurate and environmentally friendly analysis compared to other elementary detection methods. The studied plants Croton dioicus and Phoradendron villosum are native to Mexico. Both showed high levels of Cu and Ni, while their extracts present levels within the permissible range.

Crataegus oxyacantha is used in the treatment of cardiovascular diseases. The aim of this study was to evaluate the transplacental genotoxicity effect of aqueous (AE) and hydroalcoholic extract (HE) of leaves C. oxyacantha in a rat model and the quantification of malondialdehyde (MDA) in the liver. Three different doses of the AE and HE of the C. oxyacantha leaf were administered orally (500, 1000 and 2000 mg/kg) to Wistar rats during 5 days through the pregnancy term (16–21 days), and sampling in rats occurred every 24 h during the last 6 days of gestation, while only one sample was taken in neonates at birth. A sample of the mother’s and the neonate’s liver was taken for the determination of MDA. The results show that, at the hepatic level, the evaluated doses of extracts C. oxyacantha in pregnant rats and their pups did not show cytotoxicity. However, the AE and HE generated cytotoxic and genotoxic damage in the short term. On the other hand, only the AE showed a teratogenic effect. Based on these results, the AE and HE of the C. oxyacantha leaf should not be administered during pregnancy.