Delayed muscle development and impaired tissue repair are common occurrences in sheep reared for mutton. Therefore, understanding the regulatory mechanisms involved in muscle growth and development is critical for animal production. Skeletal muscle satellite cells (SMSCs) can simulate the proliferation and differentiation of muscle cells and could be induced to differentiate into myoblasts. β-hydroxy-β-methylbutyric acid (HMB) is an additive commonly used in animal production. This study examined the effect of HMB on myoblast injury repair using flow cytometry, EdU assay, RNA sequencing, Western blot, and ELISA. Our results showed that HMB could inhibit IL-17 expression and, in turn, inhibit NF-κB signaling. By acting on the downstream genes of NF-κB pathway IL-6, TNF-α and IL-1β, HMB inhibits the apoptosis and promotes the proliferation of myoblasts. The findings of this study provide insight into the mechanism by which HMB mediates myoblast injury repair in sheep.

Objective: To study the effects of synaptic vesicle glycoprotein 2B（SV2B） on proliferation, invasion and migration of human glioma cells. Methods: Bioinformatics analysis of the prognosis of malignant glioma related genes, and to establish a stable expression SV2B cell line U251 and U87, using the fluorescence microscope and image cells infected with fluorescence efficiency, further extraction cell protein and Westblot experiment, testing each SV2B protein expression, scratch test each cell migration ability, Transwell migration experiment testing each cell migration ability, Transwell invasion experiment testing each cell invasive, EdU experiment testing each cell proliferation, The cell proliferation ability was detected by plate colony formation assay. Results: Bioinformatics analysis showed that SV2B was a gene associated with poor prognosis in glioma, and high expression of SV2B protein was associated with shorter survival time in GBM patients. In the stably expressing SV2B cell lines constructed by lentivirus, overexpression of SV2B family caused the enhancement of proliferation, invasion and migration of human glioma cells. Conclusion: SV2B plays an important role in proliferation, invasion and migration of human glioma cells.